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The map label for this gene is est [H]

Identifier: 169628287

GI number: 169628287

Start: 1208608

End: 1209693

Strand: Direct

Name: est [H]

Synonym: MAB_1194

Alternate gene names: 169628287

Gene position: 1208608-1209693 (Clockwise)

Preceding gene: 169628283

Following gene: 169628288

Centisome position: 23.85

GC content: 66.94

Gene sequence:

>1086_bases
ATGACCGCACCGAAGCGCGCACCCGAATACGGGCCTGCGGGGTCTGCTAGATCCTCGCTGAGTGCCGTGAATACGCTGGT
CCGCGGCCTGCCGTTGAGTGACGGCGCCGCCGTCGAAGTGATCGAGGACGCCGCCAGCCTGGCCGGACGGCTCCTTGGCT
TCGGTGTCCGGATGACGGTGCGCACGGTACTGCAGATCGGCAGCCACGCGCCCACCTTCCCCTACCCGTTCGGGTTGATC
GAGGAGCTCGGCCGGGTGCTGGTGCCGCCGCGCGGCACGGTCAAGGCGACCGTCACCCTGCCGAACACCAACGCGCGGCT
GATCCGCGCCAAGGGTGTGGGGCCGGCCGACGGAACCGGACGCGTGGTGCTGTATCTGCATGGCGGCGCGTTCATCGTCG
GTGGCCCAAACACCCACAGCGCCATGGTGTCCACCATCTCTCAACATGCCGATGCGCCGTGTCTGGTGGTCAACTATCGG
ATGCCGCCCAAGGCCTCGCTGGATCAGGCCATCGACGATTGCCTCGACGGATACCGCTGGCTGCGCGCGCAGGGGTACGC
GGCCGAACAGATTGTGCTGGCCGGCGACTCGGCAGGGGGTTTCCTGTCCGTCGCGGTGGCCGAACGTCTGTTGGCCGAAG
ACGGTGAAGCACCGGCCGCGCTGGCCCTCATCTCGCCGCTGATCGAACTGGATCCCGCGGCCAAGGTCGCCCATGAGAAC
GCCAGGACCGATGTCATGTTGCCGCCCAACTGTTTTGAGGCGCTAGAGAAGATCCTCACCAAGGCAGGCGGTGGAATACC
GCCACAGGAGATCATCGACAAGGTCGACGGCCGCATGCCACAGACGCTGGTGCACTGCTCAGGGTCGGAGGTGCTGCTCT
ACGACGCCCGCTTGCTGGGGAAACGCCTCGCCGAGCAGGGTGTGCCCGTGGAGATCAAAATCTGGCCCGGCCAGATGCAC
GTCTTCCAGGTGGCCACCAAGGTGGTCCCCGAGGCCAAGAGATCGCTGGCACAGATCGGCCAGTACATCCGCGACGCCGT
ACCCGGGACTTTCCCGGCCGAGTTCGCCGCGCCCGCGGCGGTCTGA

Upstream 100 bases:

>100_bases
GCGGTTTGTTGCTTGTTGATAGGTATAGTCCATCGGACGCTAGCTGGCAGCTAGCAGTTAAGCGTCACAGGACACTGGCT
AGGCAAAGGAGCCTGAACAG

Downstream 100 bases:

>100_bases
TCGGCGTTCTGGGCCCTCGCCGGATGCCTGTGTGGGAGCCTCTGCGCGCGGGACACTCATCGGTCTAATAGCGTTACCTG
CATGCGCATCGCCCAGCATG

Product: putative lipase LipU

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTAPKRAPEYGPAGSARSSLSAVNTLVRGLPLSDGAAVEVIEDAASLAGRLLGFGVRMTVRTVLQIGSHAPTFPYPFGLI
EELGRVLVPPRGTVKATVTLPNTNARLIRAKGVGPADGTGRVVLYLHGGAFIVGGPNTHSAMVSTISQHADAPCLVVNYR
MPPKASLDQAIDDCLDGYRWLRAQGYAAEQIVLAGDSAGGFLSVAVAERLLAEDGEAPAALALISPLIELDPAAKVAHEN
ARTDVMLPPNCFEALEKILTKAGGGIPPQEIIDKVDGRMPQTLVHCSGSEVLLYDARLLGKRLAEQGVPVEIKIWPGQMH
VFQVATKVVPEAKRSLAQIGQYIRDAVPGTFPAEFAAPAAV

Sequences:

>Translated_361_residues
MTAPKRAPEYGPAGSARSSLSAVNTLVRGLPLSDGAAVEVIEDAASLAGRLLGFGVRMTVRTVLQIGSHAPTFPYPFGLI
EELGRVLVPPRGTVKATVTLPNTNARLIRAKGVGPADGTGRVVLYLHGGAFIVGGPNTHSAMVSTISQHADAPCLVVNYR
MPPKASLDQAIDDCLDGYRWLRAQGYAAEQIVLAGDSAGGFLSVAVAERLLAEDGEAPAALALISPLIELDPAAKVAHEN
ARTDVMLPPNCFEALEKILTKAGGGIPPQEIIDKVDGRMPQTLVHCSGSEVLLYDARLLGKRLAEQGVPVEIKIWPGQMH
VFQVATKVVPEAKRSLAQIGQYIRDAVPGTFPAEFAAPAAV
>Mature_360_residues
TAPKRAPEYGPAGSARSSLSAVNTLVRGLPLSDGAAVEVIEDAASLAGRLLGFGVRMTVRTVLQIGSHAPTFPYPFGLIE
ELGRVLVPPRGTVKATVTLPNTNARLIRAKGVGPADGTGRVVLYLHGGAFIVGGPNTHSAMVSTISQHADAPCLVVNYRM
PPKASLDQAIDDCLDGYRWLRAQGYAAEQIVLAGDSAGGFLSVAVAERLLAEDGEAPAALALISPLIELDPAAKVAHENA
RTDVMLPPNCFEALEKILTKAGGGIPPQEIIDKVDGRMPQTLVHCSGSEVLLYDARLLGKRLAEQGVPVEIKIWPGQMHV
FQVATKVVPEAKRSLAQIGQYIRDAVPGTFPAEFAAPAAV

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=131, Percent_Identity=30.5343511450382, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 37921; Mature: 37790

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPKRAPEYGPAGSARSSLSAVNTLVRGLPLSDGAAVEVIEDAASLAGRLLGFGVRMTV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RTVLQIGSHAPTFPYPFGLIEELGRVLVPPRGTVKATVTLPNTNARLIRAKGVGPADGTG
HHHHHHCCCCCCCCCCHHHHHHHHHEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCC
RVVLYLHGGAFIVGGPNTHSAMVSTISQHADAPCLVVNYRMPPKASLDQAIDDCLDGYRW
EEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LRAQGYAAEQIVLAGDSAGGFLSVAVAERLLAEDGEAPAALALISPLIELDPAAKVAHEN
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCC
ARTDVMLPPNCFEALEKILTKAGGGIPPQEIIDKVDGRMPQTLVHCSGSEVLLYDARLLG
CCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHEECCCCEEEEEHHHHHH
KRLAEQGVPVEIKIWPGQMHVFQVATKVVPEAKRSLAQIGQYIRDAVPGTFPAEFAAPAA
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
V
C
>Mature Secondary Structure 
TAPKRAPEYGPAGSARSSLSAVNTLVRGLPLSDGAAVEVIEDAASLAGRLLGFGVRMTV
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RTVLQIGSHAPTFPYPFGLIEELGRVLVPPRGTVKATVTLPNTNARLIRAKGVGPADGTG
HHHHHHCCCCCCCCCCHHHHHHHHHEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCC
RVVLYLHGGAFIVGGPNTHSAMVSTISQHADAPCLVVNYRMPPKASLDQAIDDCLDGYRW
EEEEEEECCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LRAQGYAAEQIVLAGDSAGGFLSVAVAERLLAEDGEAPAALALISPLIELDPAAKVAHEN
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCC
ARTDVMLPPNCFEALEKILTKAGGGIPPQEIIDKVDGRMPQTLVHCSGSEVLLYDARLLG
CCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHEECCCCEEEEEHHHHHH
KRLAEQGVPVEIKIWPGQMHVFQVATKVVPEAKRSLAQIGQYIRDAVPGTFPAEFAAPAA
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]