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The map label for this gene is fadD21 [H]

Identifier: 169628267

GI number: 169628267

Start: 1189109

End: 1190968

Strand: Reverse

Name: fadD21 [H]

Synonym: MAB_1174c

Alternate gene names: 169628267

Gene position: 1190968-1189109 (Counterclockwise)

Preceding gene: 169628269

Following gene: 169628266

Centisome position: 23.5

GC content: 66.77

Gene sequence:

>1860_bases
GTGAGTCGGGAGCTGGATATGTCAGACGGAGTGGCCGTTCGCCAGAGCGGGATTGAGGATTATCTCGACGTCGACGGTTT
GATCGATCTGCCTCCCGGGTCAACGCTACTGTCCAACTTTGAGCGCAATATCGCCGAGTTCGGTTCCACCGCGGCCTACC
GGTACCTCGACTTCACCCGTGATGACGATGGCGTGGCGATCGAGTTGAGTTGGGATGAGCTCAATACCCGGATGAGGGCG
ATAGGGGCCCGCTTGCAGCAGGTCACGGCGCCGGGGGATCGGGTGGCGATCCTGGCGCCGCAGGGCTTGGACTATGTGAT
CGGGTTTTTCGCGGCCATCCAGGCCGGAAACATCGCGGTGCCGTTGTTCGCCCCCGAGCTGCCCGGCCACGCCGAGCGCC
TCGACGCGGTGCTCGCCGATGCCACCCCGTCCGTCATTCTCACCACCGACGGCGCCGCCGAATCGGTGAACGCGTTCCTG
CGCAAGCTACCTCGTGCGCGCCGCCCACGGGTGATCGCGATCGACGCGGTTCCCGATGCGGTGGGTGCCACGTTCGTTCC
CGCCGCCCTGGCCACCGACGATCTGGCGTATCTGCAGTACACCTCGGGCTCGACGCGCACTCCGGCGGGCGTACAGATCA
CGCACCGCGCTGCCTGCACCAATGTGTTGCAGATGATCCTGGCCGGCGGCCTGGACATGGACATCCGCAGTGTGAGCTGG
CTGCCGCTGTATCACGACATGGGCCTGATCATGATCTTGTTTCCGCCGCTGTGCGGCGGACACATCACCTTGATGTCGCC
GTTGGCGTTCGTGCGGCGCCCCCGCCGCTGGATCAAACAACTGGCCACCGAATCCGCGTACGGGCGGGTGTTCGCCGCCG
CGCCCAACTTCGCGTTCGACCTGGCCGCCCAGCGCGGACTGCCACCGGCCGGCGAGACCTGGGATCTGTCCAATGTCGCG
GGCCTGCTCAACGGATCCGAACCGGTCACCATCGCCGCCATCGAGAAATTCACCGACGCATTCGCCCCCTACGGATTTCC
CCCGTCGGCCATCAAACCCTCCTACGGCATGGCCGAAGCCACCCTGTCGGTGGCCACCATCGGCATCCAGGACCGTCCCA
GCGTGATCTACCTGGACCGTGAACAGCTCGCCGAGGACCGCGCCGTGCCCGTCTCCGCCGAAGCGCCCACCGCGGTACCC
CACGTGTCCTGCGGTCAGGTGATCGCCAGCCAGTGGCTGGTCATCGTCGACCCGCGCACCGGAGCCGAGCTCCCCGAAGG
AGAAATCGGCGAGATCTGGCTGCACGGCGACAACATCGGCGCCGGCTACTGGGGCCGACCCCGCGAAACCGCGTCAACCT
TCCACAACCGGCTGCACTCACGCCTCGACAACAACAGCCACGCCACCGGAACCGCCGAGGACAGCCGGTGGCTACGTACC
GGGGACCTCGGGGTGTATCTGAACAACAACCTCTACGTCACCGGCCGCATCAAAGACCTCGTCATCATCGACGGACGCAA
CCACTACCCCCAGGACATCGAGGCCACCGCCTCACAAGCCTCCCCGGCCGTGCGTTCCGGATACGTCGCCGCATTCCCCG
TGGCCGCCAACCAACTCGCCGAAGCCGCCATCGCCGATACCAGTGAACGCCTGGTGATCGTCGCCGAACGCGCCCCCGGT
TCCGGTCGCGTCGAGCAAGCCCCGGTCATCGAGGCCATCCGCGCCGCCATCTCGCGCACCCACGCCCTACCCGTAGCCGA
CATCCGGCTCGTCGCCGCGGGCGCGATCCCCCGCACCACCAGCGGAAAACTCGCCCGCCGCGCCTGCCAAACCGAATACC
TCGCGGGGATGTACCGGTAG

Upstream 100 bases:

>100_bases
TGCGCGATCGACGACCTTGGAAGTTGCCGCCCGCCCCTCGCACGGCGGGTCACCTGCGAGCTAAGAATTTACTTAGTTAG
CGCAGGTAGTGTTTTCCATC

Downstream 100 bases:

>100_bases
CCCACCTCAACTGTGGGTTGAATCACACTGAGAGTCTGCCCCTTCGCGTTGAGGGCGCACCGCATCGGTGTTTGACTTAT
GTAATGGCCACTTCGAGCCC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MSRELDMSDGVAVRQSGIEDYLDVDGLIDLPPGSTLLSNFERNIAEFGSTAAYRYLDFTRDDDGVAIELSWDELNTRMRA
IGARLQQVTAPGDRVAILAPQGLDYVIGFFAAIQAGNIAVPLFAPELPGHAERLDAVLADATPSVILTTDGAAESVNAFL
RKLPRARRPRVIAIDAVPDAVGATFVPAALATDDLAYLQYTSGSTRTPAGVQITHRAACTNVLQMILAGGLDMDIRSVSW
LPLYHDMGLIMILFPPLCGGHITLMSPLAFVRRPRRWIKQLATESAYGRVFAAAPNFAFDLAAQRGLPPAGETWDLSNVA
GLLNGSEPVTIAAIEKFTDAFAPYGFPPSAIKPSYGMAEATLSVATIGIQDRPSVIYLDREQLAEDRAVPVSAEAPTAVP
HVSCGQVIASQWLVIVDPRTGAELPEGEIGEIWLHGDNIGAGYWGRPRETASTFHNRLHSRLDNNSHATGTAEDSRWLRT
GDLGVYLNNNLYVTGRIKDLVIIDGRNHYPQDIEATASQASPAVRSGYVAAFPVAANQLAEAAIADTSERLVIVAERAPG
SGRVEQAPVIEAIRAAISRTHALPVADIRLVAAGAIPRTTSGKLARRACQTEYLAGMYR

Sequences:

>Translated_619_residues
MSRELDMSDGVAVRQSGIEDYLDVDGLIDLPPGSTLLSNFERNIAEFGSTAAYRYLDFTRDDDGVAIELSWDELNTRMRA
IGARLQQVTAPGDRVAILAPQGLDYVIGFFAAIQAGNIAVPLFAPELPGHAERLDAVLADATPSVILTTDGAAESVNAFL
RKLPRARRPRVIAIDAVPDAVGATFVPAALATDDLAYLQYTSGSTRTPAGVQITHRAACTNVLQMILAGGLDMDIRSVSW
LPLYHDMGLIMILFPPLCGGHITLMSPLAFVRRPRRWIKQLATESAYGRVFAAAPNFAFDLAAQRGLPPAGETWDLSNVA
GLLNGSEPVTIAAIEKFTDAFAPYGFPPSAIKPSYGMAEATLSVATIGIQDRPSVIYLDREQLAEDRAVPVSAEAPTAVP
HVSCGQVIASQWLVIVDPRTGAELPEGEIGEIWLHGDNIGAGYWGRPRETASTFHNRLHSRLDNNSHATGTAEDSRWLRT
GDLGVYLNNNLYVTGRIKDLVIIDGRNHYPQDIEATASQASPAVRSGYVAAFPVAANQLAEAAIADTSERLVIVAERAPG
SGRVEQAPVIEAIRAAISRTHALPVADIRLVAAGAIPRTTSGKLARRACQTEYLAGMYR
>Mature_618_residues
SRELDMSDGVAVRQSGIEDYLDVDGLIDLPPGSTLLSNFERNIAEFGSTAAYRYLDFTRDDDGVAIELSWDELNTRMRAI
GARLQQVTAPGDRVAILAPQGLDYVIGFFAAIQAGNIAVPLFAPELPGHAERLDAVLADATPSVILTTDGAAESVNAFLR
KLPRARRPRVIAIDAVPDAVGATFVPAALATDDLAYLQYTSGSTRTPAGVQITHRAACTNVLQMILAGGLDMDIRSVSWL
PLYHDMGLIMILFPPLCGGHITLMSPLAFVRRPRRWIKQLATESAYGRVFAAAPNFAFDLAAQRGLPPAGETWDLSNVAG
LLNGSEPVTIAAIEKFTDAFAPYGFPPSAIKPSYGMAEATLSVATIGIQDRPSVIYLDREQLAEDRAVPVSAEAPTAVPH
VSCGQVIASQWLVIVDPRTGAELPEGEIGEIWLHGDNIGAGYWGRPRETASTFHNRLHSRLDNNSHATGTAEDSRWLRTG
DLGVYLNNNLYVTGRIKDLVIIDGRNHYPQDIEATASQASPAVRSGYVAAFPVAANQLAEAAIADTSERLVIVAERAPGS
GRVEQAPVIEAIRAAISRTHALPVADIRLVAAGAIPRTTSGKLARRACQTEYLAGMYR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=526, Percent_Identity=23.1939163498099, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI55749758, Length=504, Percent_Identity=23.8095238095238, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI225735629, Length=515, Percent_Identity=22.7184466019417, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI45827692, Length=515, Percent_Identity=22.7184466019417, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI225735625, Length=574, Percent_Identity=23.5191637630662, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI187761345, Length=432, Percent_Identity=25.2314814814815, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI187761343, Length=432, Percent_Identity=25.2314814814815, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788107, Length=623, Percent_Identity=23.916532905297, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=531, Percent_Identity=24.8587570621469, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193202679, Length=565, Percent_Identity=22.4778761061947, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654656, Length=403, Percent_Identity=23.5732009925558, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 66419; Mature: 66288

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRELDMSDGVAVRQSGIEDYLDVDGLIDLPPGSTLLSNFERNIAEFGSTAAYRYLDFTR
CCCCCCCCCCHHHHHCCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEC
DDDGVAIELSWDELNTRMRAIGARLQQVTAPGDRVAILAPQGLDYVIGFFAAIQAGNIAV
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEE
PLFAPELPGHAERLDAVLADATPSVILTTDGAAESVNAFLRKLPRARRPRVIAIDAVPDA
EEECCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCC
VGATFVPAALATDDLAYLQYTSGSTRTPAGVQITHRAACTNVLQMILAGGLDMDIRSVSW
CCCHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEE
LPLYHDMGLIMILFPPLCGGHITLMSPLAFVRRPRRWIKQLATESAYGRVFAAAPNFAFD
EEEHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEECCCCHHH
LAAQRGLPPAGETWDLSNVAGLLNGSEPVTIAAIEKFTDAFAPYGFPPSAIKPSYGMAEA
HHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHH
TLSVATIGIQDRPSVIYLDREQLAEDRAVPVSAEAPTAVPHVSCGQVIASQWLVIVDPRT
EEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCC
GAELPEGEIGEIWLHGDNIGAGYWGRPRETASTFHNRLHSRLDNNSHATGTAEDSRWLRT
CCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
GDLGVYLNNNLYVTGRIKDLVIIDGRNHYPQDIEATASQASPAVRSGYVAAFPVAANQLA
CCEEEEECCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHCCCHHHCCCEEEECHHHHHHH
EAAIADTSERLVIVAERAPGSGRVEQAPVIEAIRAAISRTHALPVADIRLVAAGAIPRTT
HHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
SGKLARRACQTEYLAGMYR
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRELDMSDGVAVRQSGIEDYLDVDGLIDLPPGSTLLSNFERNIAEFGSTAAYRYLDFTR
CCCCCCCCCHHHHHCCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEC
DDDGVAIELSWDELNTRMRAIGARLQQVTAPGDRVAILAPQGLDYVIGFFAAIQAGNIAV
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEE
PLFAPELPGHAERLDAVLADATPSVILTTDGAAESVNAFLRKLPRARRPRVIAIDAVPDA
EEECCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCC
VGATFVPAALATDDLAYLQYTSGSTRTPAGVQITHRAACTNVLQMILAGGLDMDIRSVSW
CCCHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEE
LPLYHDMGLIMILFPPLCGGHITLMSPLAFVRRPRRWIKQLATESAYGRVFAAAPNFAFD
EEEHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEECCCCHHH
LAAQRGLPPAGETWDLSNVAGLLNGSEPVTIAAIEKFTDAFAPYGFPPSAIKPSYGMAEA
HHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHH
TLSVATIGIQDRPSVIYLDREQLAEDRAVPVSAEAPTAVPHVSCGQVIASQWLVIVDPRT
EEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCC
GAELPEGEIGEIWLHGDNIGAGYWGRPRETASTFHNRLHSRLDNNSHATGTAEDSRWLRT
CCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
GDLGVYLNNNLYVTGRIKDLVIIDGRNHYPQDIEATASQASPAVRSGYVAAFPVAANQLA
CCEEEEECCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHCCCHHHCCCEEEECHHHHHHH
EAAIADTSERLVIVAERAPGSGRVEQAPVIEAIRAAISRTHALPVADIRLVAAGAIPRTT
HHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
SGKLARRACQTEYLAGMYR
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]