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The map label for this gene is pth

Identifier: 169628235

GI number: 169628235

Start: 1158407

End: 1158997

Strand: Reverse

Name: pth

Synonym: MAB_1142c

Alternate gene names: 169628235

Gene position: 1158997-1158407 (Counterclockwise)

Preceding gene: 169628236

Following gene: 169628234

Centisome position: 22.87

GC content: 65.65

Gene sequence:

>591_bases
GTGCCGGTTTTATCCGACGACGCCGTGCTCGTGGTCGGCCTGGGCAATCCCGGGCCGAACTACGCCAAGACACGGCACAA
TCTCGGATTCATGGTCGCCGATCTGTTGGCGGCCAAAGACGGCGCAACCTTCAAGCCGCACAAGAAGTCCGGCGCCGAGG
CGACAACGATTCGCCTCGGCGGCCGGGCCGTAAATCTGGCCAAGCCGCGCACGTTCATGAATACCTCCGGCCCGCAGGTC
GCCGCGCTGGCCAAGTTCTTTTCGGTACCGCCGGCGAACGTCATCGTCATGCACGACGAGCTGGATCTGGATTTCGGCAC
CGTGCGACTCAAGCTCGGCGGCGGCGAGGGCGGCCACAACGGGCTGCGCTCGGTATCGCAGTCGCTGGGCACCAAGGACT
ATCTACGGGTTCGGCTGGGCGTCGGACGCCCGCCCGGCCGTAAGGACCCGGCGGCCTTTGTGCTGGAGAACTTTGCCAAG
GCGGAGACCGATCAGGTTCCGCTGCTGTGCGAGCAGGGCGCAGACGCCGCCGAGCTGCTGATTCGCCTCGGTCTGGAGGC
AGCACAGAATCAGGTGCATGCCTGGGGTTAG

Upstream 100 bases:

>100_bases
AGCAGCCGACCTCGAGTCCGAGGGCGCCGGCGAGGAGGGTGCCGAGGCTCCCGCCGACGAGGCCGCCGAGGGCTCCACCG
AAGAGTAGGCGGAGCACTCC

Downstream 100 bases:

>100_bases
ATCCCTGGATGTGCCCGCTATGGTCTCCTCATGACCGATATCGCGGAGATCACCGGCATCAACAAGATCGGCCTACTCAG
CCTGGGGGCGTACCGACCCG

Product: peptidyl-tRNA hydrolase (PTH)

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MPVLSDDAVLVVGLGNPGPNYAKTRHNLGFMVADLLAAKDGATFKPHKKSGAEATTIRLGGRAVNLAKPRTFMNTSGPQV
AALAKFFSVPPANVIVMHDELDLDFGTVRLKLGGGEGGHNGLRSVSQSLGTKDYLRVRLGVGRPPGRKDPAAFVLENFAK
AETDQVPLLCEQGADAAELLIRLGLEAAQNQVHAWG

Sequences:

>Translated_196_residues
MPVLSDDAVLVVGLGNPGPNYAKTRHNLGFMVADLLAAKDGATFKPHKKSGAEATTIRLGGRAVNLAKPRTFMNTSGPQV
AALAKFFSVPPANVIVMHDELDLDFGTVRLKLGGGEGGHNGLRSVSQSLGTKDYLRVRLGVGRPPGRKDPAAFVLENFAK
AETDQVPLLCEQGADAAELLIRLGLEAAQNQVHAWG
>Mature_195_residues
PVLSDDAVLVVGLGNPGPNYAKTRHNLGFMVADLLAAKDGATFKPHKKSGAEATTIRLGGRAVNLAKPRTFMNTSGPQVA
ALAKFFSVPPANVIVMHDELDLDFGTVRLKLGGGEGGHNGLRSVSQSLGTKDYLRVRLGVGRPPGRKDPAAFVLENFAKA
ETDQVPLLCEQGADAAELLIRLGLEAAQNQVHAWG

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=37.5, Blast_Score=101, Evalue=4e-22,
Organism=Escherichia coli, GI1787455, Length=187, Percent_Identity=41.1764705882353, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321983, Length=176, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_MYCA9 (B1MKD8)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701884.1
- ProteinModelPortal:   B1MKD8
- SMR:   B1MKD8
- EnsemblBacteria:   EBMYCT00000004877
- GeneID:   5963667
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1142c
- GeneTree:   EBGT00050000017462
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- BioCyc:   MABS561007:MAB_1142C-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20688; Mature: 20557

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLSDDAVLVVGLGNPGPNYAKTRHNLGFMVADLLAAKDGATFKPHKKSGAEATTIRLG
CCCCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
GRAVNLAKPRTFMNTSGPQVAALAKFFSVPPANVIVMHDELDLDFGTVRLKLGGGEGGHN
CEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCCCHH
GLRSVSQSLGTKDYLRVRLGVGRPPGRKDPAAFVLENFAKAETDQVPLLCEQGADAAELL
HHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHH
IRLGLEAAQNQVHAWG
HHHCHHHHHCCEECCC
>Mature Secondary Structure 
PVLSDDAVLVVGLGNPGPNYAKTRHNLGFMVADLLAAKDGATFKPHKKSGAEATTIRLG
CCCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
GRAVNLAKPRTFMNTSGPQVAALAKFFSVPPANVIVMHDELDLDFGTVRLKLGGGEGGHN
CEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCCCHH
GLRSVSQSLGTKDYLRVRLGVGRPPGRKDPAAFVLENFAKAETDQVPLLCEQGADAAELL
HHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHH
IRLGLEAAQNQVHAWG
HHHCHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA