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The map label for this gene is metG [H]

Identifier: 169628221

GI number: 169628221

Start: 1139038

End: 1140609

Strand: Reverse

Name: metG [H]

Synonym: MAB_1128c

Alternate gene names: 169628221

Gene position: 1140609-1139038 (Counterclockwise)

Preceding gene: 169628225

Following gene: 169628220

Centisome position: 22.51

GC content: 64.25

Gene sequence:

>1572_bases
ATGCCTGAATCATTGGATCCGTTCTACATCACCACCGCCATCGCGTATCCGAACGGCGAACCGCACATCGGACACGCATA
TGAGTACGTCGCGACCGACGCCGTCGCCCGCTTCAAACGGCTGGACGGCTTCGACGTGCGCTATCTCACGGGCACCGATG
AGCACGGTTTGAAGGTGGCGCAGACGGCCGCCGCGGCCGGAATCCCCACCGCGCAGCTGGCCCGGCACAATTCCGATGTG
TTCGAGGCGCTACAGCGCACACTCAACATCTCCTTCGACCGGTTCATCCGCACGACCGACCCGGATCACTACGAGGCCTC
GAAGGCCATCTGGCGCAAGATGGCCGATAACGGTGACATCTATTTGGACACCTACTCCGGCTGGTACTCGGTGCGCGACG
AGGCGTACTACACCGACGCGGAGACCACGGTGGCCCCCGACGGCACCCGCACCGCCACCGCGACGGGGACTCCGGTCGAC
TGGACCGAGGAGCATTCCTATTTCTTCAGGCTCTCGGCGTACCAGGACAAGCTGCTGGCGCACTACGAGGCACATCCCGA
GTTCATCGGCCCCGATGTGCGACGCAACGAGGTGGTCAGCTTCGTCTCCGGCGGGCTGCGCGACCTGTCCATCTCACGGA
CCACCTTCGACTGGGGCGTGCCGGTCCCCGACAACCCCGAACATGTCATGTACGTGTGGGTGGACGCGCTGACCAACTAC
CTGACGGGGGTCGGCTACCCGGATACCGATTCGGAGCTGTTCGGGCGGTACTGGCCCGCCAACATCCACATGATCGGCAA
GGACATCATCCGGTTCCACACCGTGTACTGGCCCGCCTTCCTGATGTCTGCCGGGATCGCGCTGCCCCAGCGGGTCTTCG
CGCACGGCTTCCTGACCAACAAGGGCGAGAAGATGAGCAAGTCGATCGGCAACGTCGTCGACCCCATCAACCTCGTCAAC
ACCTTTGGCCTGGATCAGGTGCGCTACTTTCTGCTGCGCGAGGTGCCGTTCGGGCAGGACGGCAGCTATACCGAAGAAGG
CCTGATCGGACGGATCAACGCCGACCTGGCCAACGAGCTGGGCAATCTGGCGCAGCGGTCACTGTCGATGGTGGCCAAGA
ACCTGGGCGGAATCGTGCCGGAGCCGGGTGAATTCGCCCCCGAGGACACCGAACTCCTCGCCGCCGCCGACACGCTGCTG
GAGCAGGCCCGGACGCATTTCGAGGTGCCCGCCATGCATCTGGCGCTGGAAGCGATCTGGCAGGTGCTGGGGGCGAGCAA
TCGGTACTTCTCGGCACAGGAGCCCTGGGTGCTGGCCAAGAGCTTCCAGACAGACGGATCCGCGGCGGACCAAGAGCGCT
TCGGCACCGTGCTGTACACCACGCTGGAAGTGGTTCGAATTGTCGCGCTGTTGGTGCAGCCGGTGATGCCCGAGTCGGCA
GCCAAGCTGCTGGATCTGTTGGGCCAGGCCGACGATCAGCGGGACTTCGCGGCCATCGGAACACGGTTGAAGCCCGGCGT
GAGCCTGCCCGCGCCGACGGGTGTGTTCCCGAAATATCAGGTGCCCAAGTAG

Upstream 100 bases:

>100_bases
TCGGGTGGGCGGGAACGAATGCGGTCGGGTGGGGCCTGCCTGTTATTGCCGGGCGTCATCAGGTATCCATTCTATTTGGA
TAGATACAGTTGCTCCCACC

Downstream 100 bases:

>100_bases
GCCGCCGAATGTGAGCCCGGTCACGGTCCGTCACAGATTTCGTCGCCCCGGTGTCTTAAGGGCATCAGCGACGGAAAGGA
AGTCACCGAAATGACCGAGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPESLDPFYITTAIAYPNGEPHIGHAYEYVATDAVARFKRLDGFDVRYLTGTDEHGLKVAQTAAAAGIPTAQLARHNSDV
FEALQRTLNISFDRFIRTTDPDHYEASKAIWRKMADNGDIYLDTYSGWYSVRDEAYYTDAETTVAPDGTRTATATGTPVD
WTEEHSYFFRLSAYQDKLLAHYEAHPEFIGPDVRRNEVVSFVSGGLRDLSISRTTFDWGVPVPDNPEHVMYVWVDALTNY
LTGVGYPDTDSELFGRYWPANIHMIGKDIIRFHTVYWPAFLMSAGIALPQRVFAHGFLTNKGEKMSKSIGNVVDPINLVN
TFGLDQVRYFLLREVPFGQDGSYTEEGLIGRINADLANELGNLAQRSLSMVAKNLGGIVPEPGEFAPEDTELLAAADTLL
EQARTHFEVPAMHLALEAIWQVLGASNRYFSAQEPWVLAKSFQTDGSAADQERFGTVLYTTLEVVRIVALLVQPVMPESA
AKLLDLLGQADDQRDFAAIGTRLKPGVSLPAPTGVFPKYQVPK

Sequences:

>Translated_523_residues
MPESLDPFYITTAIAYPNGEPHIGHAYEYVATDAVARFKRLDGFDVRYLTGTDEHGLKVAQTAAAAGIPTAQLARHNSDV
FEALQRTLNISFDRFIRTTDPDHYEASKAIWRKMADNGDIYLDTYSGWYSVRDEAYYTDAETTVAPDGTRTATATGTPVD
WTEEHSYFFRLSAYQDKLLAHYEAHPEFIGPDVRRNEVVSFVSGGLRDLSISRTTFDWGVPVPDNPEHVMYVWVDALTNY
LTGVGYPDTDSELFGRYWPANIHMIGKDIIRFHTVYWPAFLMSAGIALPQRVFAHGFLTNKGEKMSKSIGNVVDPINLVN
TFGLDQVRYFLLREVPFGQDGSYTEEGLIGRINADLANELGNLAQRSLSMVAKNLGGIVPEPGEFAPEDTELLAAADTLL
EQARTHFEVPAMHLALEAIWQVLGASNRYFSAQEPWVLAKSFQTDGSAADQERFGTVLYTTLEVVRIVALLVQPVMPESA
AKLLDLLGQADDQRDFAAIGTRLKPGVSLPAPTGVFPKYQVPK
>Mature_522_residues
PESLDPFYITTAIAYPNGEPHIGHAYEYVATDAVARFKRLDGFDVRYLTGTDEHGLKVAQTAAAAGIPTAQLARHNSDVF
EALQRTLNISFDRFIRTTDPDHYEASKAIWRKMADNGDIYLDTYSGWYSVRDEAYYTDAETTVAPDGTRTATATGTPVDW
TEEHSYFFRLSAYQDKLLAHYEAHPEFIGPDVRRNEVVSFVSGGLRDLSISRTTFDWGVPVPDNPEHVMYVWVDALTNYL
TGVGYPDTDSELFGRYWPANIHMIGKDIIRFHTVYWPAFLMSAGIALPQRVFAHGFLTNKGEKMSKSIGNVVDPINLVNT
FGLDQVRYFLLREVPFGQDGSYTEEGLIGRINADLANELGNLAQRSLSMVAKNLGGIVPEPGEFAPEDTELLAAADTLLE
QARTHFEVPAMHLALEAIWQVLGASNRYFSAQEPWVLAKSFQTDGSAADQERFGTVLYTTLEVVRIVALLVQPVMPESAA
KLLDLLGQADDQRDFAAIGTRLKPGVSLPAPTGVFPKYQVPK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=514, Percent_Identity=38.3268482490272, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI14043022, Length=527, Percent_Identity=26.7552182163188, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI1788432, Length=513, Percent_Identity=26.7056530214425, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1786861, Length=179, Percent_Identity=24.5810055865922, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=531, Percent_Identity=35.969868173258, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI71992781, Length=426, Percent_Identity=34.7417840375587, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=533, Percent_Identity=26.4540337711069, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321610, Length=542, Percent_Identity=31.1808118081181, Blast_Score=292, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6321704, Length=521, Percent_Identity=22.8406909788868, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323414, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358483, Length=501, Percent_Identity=38.9221556886228, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655311, Length=552, Percent_Identity=25, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355409, Length=179, Percent_Identity=26.2569832402235, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58107; Mature: 57976

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESLDPFYITTAIAYPNGEPHIGHAYEYVATDAVARFKRLDGFDVRYLTGTDEHGLKVA
CCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
QTAAAAGIPTAQLARHNSDVFEALQRTLNISFDRFIRTTDPDHYEASKAIWRKMADNGDI
HHHHHCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHCCCCCE
YLDTYSGWYSVRDEAYYTDAETTVAPDGTRTATATGTPVDWTEEHSYFFRLSAYQDKLLA
EEEECCCEEEECCCEEEECCCCEECCCCCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHH
HYEAHPEFIGPDVRRNEVVSFVSGGLRDLSISRTTFDWGVPVPDNPEHVMYVWVDALTNY
HHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEHHHHHHH
LTGVGYPDTDSELFGRYWPANIHMIGKDIIRFHTVYWPAFLMSAGIALPQRVFAHGFLTN
HHCCCCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
KGEKMSKSIGNVVDPINLVNTFGLDQVRYFLLREVPFGQDGSYTEEGLIGRINADLANEL
CCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHH
GNLAQRSLSMVAKNLGGIVPEPGEFAPEDTELLAAADTLLEQARTHFEVPAMHLALEAIW
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QVLGASNRYFSAQEPWVLAKSFQTDGSAADQERFGTVLYTTLEVVRIVALLVQPVMPESA
HHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AKLLDLLGQADDQRDFAAIGTRLKPGVSLPAPTGVFPKYQVPK
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PESLDPFYITTAIAYPNGEPHIGHAYEYVATDAVARFKRLDGFDVRYLTGTDEHGLKVA
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
QTAAAAGIPTAQLARHNSDVFEALQRTLNISFDRFIRTTDPDHYEASKAIWRKMADNGDI
HHHHHCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHHCCCCCE
YLDTYSGWYSVRDEAYYTDAETTVAPDGTRTATATGTPVDWTEEHSYFFRLSAYQDKLLA
EEEECCCEEEECCCEEEECCCCEECCCCCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHH
HYEAHPEFIGPDVRRNEVVSFVSGGLRDLSISRTTFDWGVPVPDNPEHVMYVWVDALTNY
HHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEHHHHHHH
LTGVGYPDTDSELFGRYWPANIHMIGKDIIRFHTVYWPAFLMSAGIALPQRVFAHGFLTN
HHCCCCCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
KGEKMSKSIGNVVDPINLVNTFGLDQVRYFLLREVPFGQDGSYTEEGLIGRINADLANEL
CCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHH
GNLAQRSLSMVAKNLGGIVPEPGEFAPEDTELLAAADTLLEQARTHFEVPAMHLALEAIW
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QVLGASNRYFSAQEPWVLAKSFQTDGSAADQERFGTVLYTTLEVVRIVALLVQPVMPESA
HHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AKLLDLLGQADDQRDFAAIGTRLKPGVSLPAPTGVFPKYQVPK
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]