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The map label for this gene is purN [H]

Identifier: 169628158

GI number: 169628158

Start: 1073331

End: 1073969

Strand: Direct

Name: purN [H]

Synonym: MAB_1063

Alternate gene names: 169628158

Gene position: 1073331-1073969 (Clockwise)

Preceding gene: 169628157

Following gene: 169628159

Centisome position: 21.18

GC content: 66.2

Gene sequence:

>639_bases
GTGTCGGTGCCCAGTCAGTCAGTCGAGATCGCGCCATCTGCTCCCGCGCGGGTCGTGGTCTTGGCCTCCGGTACCGGTAC
CCTGCTGCGTTCCCTGCTGGACGCGGCCACCGGCGATTTTCCCGCGCGCATCGTCGCGGTGGGCACCGACCGACCGTGTC
CCGCCCTCGATATCGCTGCCGACGCACAGCTCCCCAGCTACATGGTGCGACTCGGCGATTACGACTCCCGCGAACAATGG
GACGCGGCGATCGCCGAGGCCACCGCGGTGCACCGCCCCGACCTCGTGGTGTCCGCCGGGTTTATGAAGATTCTTGGACC
GCAGTTCCTTTCGCAGTTCTTGGGCCGCGTGATCAATACCCACCCTGCGCTGCTGCCGTCCTTTCCCGGCGCGCACGCCG
TGCCCGAGGCCTTGGCGCATGGCGTCAAGATCACCGGCTGCACCGTGCATCTGGTCGACGCGGGGATGGACACCGGGCCG
ATCCTTGCCCAGCAGGCCGTCCCGGTAGACCGGGACGACGACGAGGCGAGTCTGCATGAACGCATCAAGGTAGTGGAACG
GACCCTGCTGGTAGATGTGCTGGCCGCGGTGGCCACCAAAGGTTTGACATGGAACGGAAGAAGGGCCTCCATCAAGTGA

Upstream 100 bases:

>100_bases
CCGAATCAGAACCCGAAGCCGACACGGATGAGGGTCCCGAGATCGACCCCAGCGACCCTCCGGAATCGGCCAAGTGACCA
GCCCGGCTACGCTCAGCGGC

Downstream 100 bases:

>100_bases
GTACGCAGCGACCCGTACGTCGGGCATTGATCAGCGTTTACGACAAGACCGGGCTGGAGGAGCTCGCCAGCGGTCTCCAT
GCGGCCGGTGTGGAGCTGGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSVPSQSVEIAPSAPARVVVLASGTGTLLRSLLDAATGDFPARIVAVGTDRPCPALDIAADAQLPSYMVRLGDYDSREQW
DAAIAEATAVHRPDLVVSAGFMKILGPQFLSQFLGRVINTHPALLPSFPGAHAVPEALAHGVKITGCTVHLVDAGMDTGP
ILAQQAVPVDRDDDEASLHERIKVVERTLLVDVLAAVATKGLTWNGRRASIK

Sequences:

>Translated_212_residues
MSVPSQSVEIAPSAPARVVVLASGTGTLLRSLLDAATGDFPARIVAVGTDRPCPALDIAADAQLPSYMVRLGDYDSREQW
DAAIAEATAVHRPDLVVSAGFMKILGPQFLSQFLGRVINTHPALLPSFPGAHAVPEALAHGVKITGCTVHLVDAGMDTGP
ILAQQAVPVDRDDDEASLHERIKVVERTLLVDVLAAVATKGLTWNGRRASIK
>Mature_211_residues
SVPSQSVEIAPSAPARVVVLASGTGTLLRSLLDAATGDFPARIVAVGTDRPCPALDIAADAQLPSYMVRLGDYDSREQWD
AAIAEATAVHRPDLVVSAGFMKILGPQFLSQFLGRVINTHPALLPSFPGAHAVPEALAHGVKITGCTVHLVDAGMDTGPI
LAQQAVPVDRDDDEASLHERIKVVERTLLVDVLAAVATKGLTWNGRRASIK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=40.2985074626866, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=40.2985074626866, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=40.2985074626866, Blast_Score=130, Evalue=7e-31,
Organism=Escherichia coli, GI1788846, Length=184, Percent_Identity=34.7826086956522, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787483, Length=213, Percent_Identity=31.4553990610329, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=38.2978723404255, Blast_Score=120, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320616, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582400, Length=174, Percent_Identity=42.5287356321839, Blast_Score=137, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22311; Mature: 22180

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPSQSVEIAPSAPARVVVLASGTGTLLRSLLDAATGDFPARIVAVGTDRPCPALDIAA
CCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
DAQLPSYMVRLGDYDSREQWDAAIAEATAVHRPDLVVSAGFMKILGPQFLSQFLGRVINT
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCHHHHHHHHHHHHHC
HPALLPSFPGAHAVPEALAHGVKITGCTVHLVDAGMDTGPILAQQAVPVDRDDDEASLHE
CCCCCCCCCCCHHHHHHHHCCEEEECEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHH
RIKVVERTLLVDVLAAVATKGLTWNGRRASIK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SVPSQSVEIAPSAPARVVVLASGTGTLLRSLLDAATGDFPARIVAVGTDRPCPALDIAA
CCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
DAQLPSYMVRLGDYDSREQWDAAIAEATAVHRPDLVVSAGFMKILGPQFLSQFLGRVINT
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCHHHHHHHHHHHHHC
HPALLPSFPGAHAVPEALAHGVKITGCTVHLVDAGMDTGPILAQQAVPVDRDDDEASLHE
CCCCCCCCCCCHHHHHHHHCCEEEECEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHH
RIKVVERTLLVDVLAAVATKGLTWNGRRASIK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]