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The map label for this gene is tcmP [H]

Identifier: 169628060

GI number: 169628060

Start: 971725

End: 972558

Strand: Reverse

Name: tcmP [H]

Synonym: MAB_0963c

Alternate gene names: 169628060

Gene position: 972558-971725 (Counterclockwise)

Preceding gene: 169628065

Following gene: 169628058

Centisome position: 19.19

GC content: 63.07

Gene sequence:

>834_bases
ATGTTGGTTCGAGTGACGGACCCAGTGAAAATCACGCTGACCGGGGCTCCCGAGACGATGCTGGCCACCCTGTACGGCAG
GGCGCAGGACGCCACATCACCCGATTCGGTTCTTCATGACCACTACGCCGCCGAGGCCGTCGCGCGCATCGACTACGACT
TCTCCAAGACCAAGATCAAGGGCACCCAGGCAATCGGCGTGGCGTTGCGTGCCCGTCAACTCGACGACTGGACCTCGGAG
TTTCTCACCGCACACCATGAGGCAACGGTGCTGCACCTGGCCTGCGGCCTGGATACCCGGGTCTTCCGGATCAACCCATC
CGAGATGGTGCGCTGGGCCGATGTCGACTATCCCGATGTGATCGCCCTGCGCCGCGCACTGCTCCCGTGCCGCGACGGCG
ATTACCGGATGATCGGAAGTTCGGTCAGCGAGGCGACGTGGCTGGACAGCATCCCGGCCGACCGTCCCACTCTCGCGGTG
TTCGAGGGTTTGACGATGTACCTGACCGAGGCCGAGGGGCAGTCACTCATCCGGCGTATCACCGGCCGATTCCCCAGTGG
TCAATTGGCTTTCGACTGCTACGGCAGCATCGGCATCAAACTACAGAAATTGGTGCCGGCGGTGCGCAACGCGGGAGCCA
CACTGCATTGGGGAATCGACGATCCCGGACAGATCGAGGCGCTGCACCCGGGCCTGCGCTGCGTCAGCGATCTGCGCAGT
ACCGATGTGGCGGGTGAGGAACACCTGCCACTATCCGGCCGCATCCAGATGAAAGTCATCTCCCACATCCCCACCCTGCG
CGACATCGGCAGGATCATGCGGTTTTCCTTCTAG

Upstream 100 bases:

>100_bases
CCAACAGGCTCTTCAGCTTGTCGATGAGGCCCTTGAAATCAGCCATGAGCTTGTTCTCCTTCGTCTGGGACGGCGCAACT
GTAATACGAGATCGGGGCGT

Downstream 100 bases:

>100_bases
CCCTAACCTCGCGAGCAGACGAGGGGCGGTCAGTCGTCTCCCGTGGCCCAGAAGTGCCGGGGCTGGCCGGACCACTTGCC
CTGCAGCCACCACACCGCTT

Product: putative polyketide synthase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLVRVTDPVKITLTGAPETMLATLYGRAQDATSPDSVLHDHYAAEAVARIDYDFSKTKIKGTQAIGVALRARQLDDWTSE
FLTAHHEATVLHLACGLDTRVFRINPSEMVRWADVDYPDVIALRRALLPCRDGDYRMIGSSVSEATWLDSIPADRPTLAV
FEGLTMYLTEAEGQSLIRRITGRFPSGQLAFDCYGSIGIKLQKLVPAVRNAGATLHWGIDDPGQIEALHPGLRCVSDLRS
TDVAGEEHLPLSGRIQMKVISHIPTLRDIGRIMRFSF

Sequences:

>Translated_277_residues
MLVRVTDPVKITLTGAPETMLATLYGRAQDATSPDSVLHDHYAAEAVARIDYDFSKTKIKGTQAIGVALRARQLDDWTSE
FLTAHHEATVLHLACGLDTRVFRINPSEMVRWADVDYPDVIALRRALLPCRDGDYRMIGSSVSEATWLDSIPADRPTLAV
FEGLTMYLTEAEGQSLIRRITGRFPSGQLAFDCYGSIGIKLQKLVPAVRNAGATLHWGIDDPGQIEALHPGLRCVSDLRS
TDVAGEEHLPLSGRIQMKVISHIPTLRDIGRIMRFSF
>Mature_277_residues
MLVRVTDPVKITLTGAPETMLATLYGRAQDATSPDSVLHDHYAAEAVARIDYDFSKTKIKGTQAIGVALRARQLDDWTSE
FLTAHHEATVLHLACGLDTRVFRINPSEMVRWADVDYPDVIALRRALLPCRDGDYRMIGSSVSEATWLDSIPADRPTLAV
FEGLTMYLTEAEGQSLIRRITGRFPSGQLAFDCYGSIGIKLQKLVPAVRNAGATLHWGIDDPGQIEALHPGLRCVSDLRS
TDVAGEEHLPLSGRIQMKVISHIPTLRDIGRIMRFSF

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: COG3315

COG function: function code Q; O-Methyltransferase involved in polyketide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 30610; Mature: 30610

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVRVTDPVKITLTGAPETMLATLYGRAQDATSPDSVLHDHYAAEAVARIDYDFSKTKIK
CEEEECCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEC
GTQAIGVALRARQLDDWTSEFLTAHHEATVLHLACGLDTRVFRINPSEMVRWADVDYPDV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECHHHHEEECCCCCHHH
IALRRALLPCRDGDYRMIGSSVSEATWLDSIPADRPTLAVFEGLTMYLTEAEGQSLIRRI
HHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCCCCHHHHHCCEEEEEECCCHHHHHHHH
TGRFPSGQLAFDCYGSIGIKLQKLVPAVRNAGATLHWGIDDPGQIEALHPGLRCVSDLRS
HCCCCCCCEEEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCCCEEEECHHHHHHHHHHH
TDVAGEEHLPLSGRIQMKVISHIPTLRDIGRIMRFSF
CCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLVRVTDPVKITLTGAPETMLATLYGRAQDATSPDSVLHDHYAAEAVARIDYDFSKTKIK
CEEEECCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEC
GTQAIGVALRARQLDDWTSEFLTAHHEATVLHLACGLDTRVFRINPSEMVRWADVDYPDV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECHHHHEEECCCCCHHH
IALRRALLPCRDGDYRMIGSSVSEATWLDSIPADRPTLAVFEGLTMYLTEAEGQSLIRRI
HHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCCCCHHHHHCCEEEEEECCCHHHHHHHH
TGRFPSGQLAFDCYGSIGIKLQKLVPAVRNAGATLHWGIDDPGQIEALHPGLRCVSDLRS
HCCCCCCCEEEEECCCCCEEHHHHHHHHHCCCCEEEECCCCCCCEEEECHHHHHHHHHHH
TDVAGEEHLPLSGRIQMKVISHIPTLRDIGRIMRFSF
CCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]