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The map label for this gene is stp [H]

Identifier: 169628042

GI number: 169628042

Start: 952177

End: 953589

Strand: Direct

Name: stp [H]

Synonym: MAB_0945

Alternate gene names: 169628042

Gene position: 952177-953589 (Clockwise)

Preceding gene: 169628041

Following gene: 169628043

Centisome position: 18.79

GC content: 65.96

Gene sequence:

>1413_bases
ATGGAAGCGGCCGTCGGTGCCCTGAGTGAGCGCCGCAAGATCGTCATCTTGGGGTCTTGCTGCCTGAGCCTGCTCATCGT
GTCGATGGACTCCACCATCGTGAACGTCGCCCTGCCGACCATCCGGGCGGATTTCGGCGCGACTACTTCGCAGCTGCAGT
GGGTTATTGATATCTACACCCTGGGCCTGGCCTCGCTGCTGATGCTTTCGGGCGCCGCGGGGGACCGGCTGGGACGCCGG
AAGGTCTTTCACCTCGGGCTGTCGATCTTCGCGATCGGCTCGCTGCTCTGCAGTGTGGCGCCCACCGTCGGGCTGCTCAT
CGCCGCCCGTCTGCTCCAGGCGGTGGGTGGATCGATGCTGAATCCGGTTGCGCTCTCCATCATTTCGCAGGTTTTCACCG
GCCGCGTGGAGCGCGCCAGGGCGCTGGGGTTCTGGGGTGCCGTGGTGGGCATATCGATGGCGCTAGGCCCGATCGTCGGG
GGGTTGCTGATCCAATGGGTGGGCTGGCGCGCCGTGTTCTGGATCAACCTGCCCGTATGCGTGCTGGCCTTCGTGCTGAC
GGCGGTCTTTGTGCCGGAGACCAAGTCGGCCACCATGCGCGACATCGACCCCGTAGGCCAGGCACTTGCGGTGCTCTTCC
TGTTCGGCATCGTGTTCGCCCTGATCGAAGGGCCATCGCTGGGGTGGGCGACGCCCGGACTGATCGTGGTGCTGGCAGTC
GCGCTGGCGGCGTTCGCGGTGTTCCTCCGCTATGAGTCCCGGCGGCGGCACCCGTTCATCGATCTACGTTTCTTCCGCAG
CATCCCATTTGCCTCGGCCACTCTTACGGCGATATGCGCGTGCATGGCGTGGAGTGCGTTCTTATTCACCATGTCGCTGT
ATCTGCAGGGGCAGCGCCACTTCTCGGCAATGCACACCGGCCTGATCTATCTCCCGATTGCCGTCGGAGCGCTGGTGTTC
TCCCCGATATCGGGCCGAATGGTGGGCCGATTCGGCGCACGGCCGTCGCTGCTCGCGGCGGGCACCCTGTTCTTGACCGC
TTCGCTGCTCTTGACGAGGGTGTCGGAGGCGACGCCAATCTGGGAGCTACTGGTGACCTTCGCGATTTTCGGTGCCGGGT
TCGCGATGGTGAATGCGCCGATCACCAACGCCGCCGTCAGCGGTATGCCGCTGGACCGCGCGGGCGCGGCCTCCGCGGTG
ACGTCCACCAGTCGCCAGGTCGGGGTGAGTATCGGTGTGGCGCTGTGTGGTTCGATCGGGACTCCCGCCCTGTGGTTCAT
GTGCGCGGGGCTGGGCCTGCTCATCGCTACGCTGGGGATTGTGTCCACGTCACCGGCCGCGGTGCGGTCCGCGCAGCGGC
TCGCACCGCTCATCGAGGGCACCACGGCGCGGGAGATGTCTCGTGTCGGATAG

Upstream 100 bases:

>100_bases
ATCTGACACTGTGATCTGACACAGTGATCTGACACAGTGATCTGACACTGTGACGCAAGTCTGTTTGCATTGCTAATAGT
TGTGGTTTCACAATGGTTTC

Downstream 100 bases:

>100_bases
ATCGCTCGCGGACGAAGTATGGCGCAGCATGGCAAGCCTGGTGCTCGACAATCGTGACAGCTGGAGACGCACCGTCGTAG
AGACGACAGGCCTCCCGTTC

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MEAAVGALSERRKIVILGSCCLSLLIVSMDSTIVNVALPTIRADFGATTSQLQWVIDIYTLGLASLLMLSGAAGDRLGRR
KVFHLGLSIFAIGSLLCSVAPTVGLLIAARLLQAVGGSMLNPVALSIISQVFTGRVERARALGFWGAVVGISMALGPIVG
GLLIQWVGWRAVFWINLPVCVLAFVLTAVFVPETKSATMRDIDPVGQALAVLFLFGIVFALIEGPSLGWATPGLIVVLAV
ALAAFAVFLRYESRRRHPFIDLRFFRSIPFASATLTAICACMAWSAFLFTMSLYLQGQRHFSAMHTGLIYLPIAVGALVF
SPISGRMVGRFGARPSLLAAGTLFLTASLLLTRVSEATPIWELLVTFAIFGAGFAMVNAPITNAAVSGMPLDRAGAASAV
TSTSRQVGVSIGVALCGSIGTPALWFMCAGLGLLIATLGIVSTSPAAVRSAQRLAPLIEGTTAREMSRVG

Sequences:

>Translated_470_residues
MEAAVGALSERRKIVILGSCCLSLLIVSMDSTIVNVALPTIRADFGATTSQLQWVIDIYTLGLASLLMLSGAAGDRLGRR
KVFHLGLSIFAIGSLLCSVAPTVGLLIAARLLQAVGGSMLNPVALSIISQVFTGRVERARALGFWGAVVGISMALGPIVG
GLLIQWVGWRAVFWINLPVCVLAFVLTAVFVPETKSATMRDIDPVGQALAVLFLFGIVFALIEGPSLGWATPGLIVVLAV
ALAAFAVFLRYESRRRHPFIDLRFFRSIPFASATLTAICACMAWSAFLFTMSLYLQGQRHFSAMHTGLIYLPIAVGALVF
SPISGRMVGRFGARPSLLAAGTLFLTASLLLTRVSEATPIWELLVTFAIFGAGFAMVNAPITNAAVSGMPLDRAGAASAV
TSTSRQVGVSIGVALCGSIGTPALWFMCAGLGLLIATLGIVSTSPAAVRSAQRLAPLIEGTTAREMSRVG
>Mature_470_residues
MEAAVGALSERRKIVILGSCCLSLLIVSMDSTIVNVALPTIRADFGATTSQLQWVIDIYTLGLASLLMLSGAAGDRLGRR
KVFHLGLSIFAIGSLLCSVAPTVGLLIAARLLQAVGGSMLNPVALSIISQVFTGRVERARALGFWGAVVGISMALGPIVG
GLLIQWVGWRAVFWINLPVCVLAFVLTAVFVPETKSATMRDIDPVGQALAVLFLFGIVFALIEGPSLGWATPGLIVVLAV
ALAAFAVFLRYESRRRHPFIDLRFFRSIPFASATLTAICACMAWSAFLFTMSLYLQGQRHFSAMHTGLIYLPIAVGALVF
SPISGRMVGRFGARPSLLAAGTLFLTASLLLTRVSEATPIWELLVTFAIFGAGFAMVNAPITNAAVSGMPLDRAGAASAV
TSTSRQVGVSIGVALCGSIGTPALWFMCAGLGLLIATLGIVSTSPAAVRSAQRLAPLIEGTTAREMSRVG

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=393, Percent_Identity=27.4809160305344, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=24.8826291079812, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788710, Length=393, Percent_Identity=29.2620865139949, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789042, Length=418, Percent_Identity=28.2296650717703, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788392, Length=394, Percent_Identity=28.6802030456853, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=31.4814814814815, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=457, Percent_Identity=24.2888402625821, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323735, Length=326, Percent_Identity=27.9141104294479, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=459, Percent_Identity=24.400871459695, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=149, Percent_Identity=28.8590604026846, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=327, Percent_Identity=24.4648318042813, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=168, Percent_Identity=27.3809523809524, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=251, Percent_Identity=25.0996015936255, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=154, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49343; Mature: 49343

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAAVGALSERRKIVILGSCCLSLLIVSMDSTIVNVALPTIRADFGATTSQLQWVIDIYT
CCCHHHHHHCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LGLASLLMLSGAAGDRLGRRKVFHLGLSIFAIGSLLCSVAPTVGLLIAARLLQAVGGSML
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NPVALSIISQVFTGRVERARALGFWGAVVGISMALGPIVGGLLIQWVGWRAVFWINLPVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHH
VLAFVLTAVFVPETKSATMRDIDPVGQALAVLFLFGIVFALIEGPSLGWATPGLIVVLAV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ALAAFAVFLRYESRRRHPFIDLRFFRSIPFASATLTAICACMAWSAFLFTMSLYLQGQRH
HHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSAMHTGLIYLPIAVGALVFSPISGRMVGRFGARPSLLAAGTLFLTASLLLTRVSEATPI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
WELLVTFAIFGAGFAMVNAPITNAAVSGMPLDRAGAASAVTSTSRQVGVSIGVALCGSIG
HHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TPALWFMCAGLGLLIATLGIVSTSPAAVRSAQRLAPLIEGTTAREMSRVG
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MEAAVGALSERRKIVILGSCCLSLLIVSMDSTIVNVALPTIRADFGATTSQLQWVIDIYT
CCCHHHHHHCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LGLASLLMLSGAAGDRLGRRKVFHLGLSIFAIGSLLCSVAPTVGLLIAARLLQAVGGSML
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NPVALSIISQVFTGRVERARALGFWGAVVGISMALGPIVGGLLIQWVGWRAVFWINLPVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHH
VLAFVLTAVFVPETKSATMRDIDPVGQALAVLFLFGIVFALIEGPSLGWATPGLIVVLAV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ALAAFAVFLRYESRRRHPFIDLRFFRSIPFASATLTAICACMAWSAFLFTMSLYLQGQRH
HHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSAMHTGLIYLPIAVGALVFSPISGRMVGRFGARPSLLAAGTLFLTASLLLTRVSEATPI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
WELLVTFAIFGAGFAMVNAPITNAAVSGMPLDRAGAASAVTSTSRQVGVSIGVALCGSIG
HHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TPALWFMCAGLGLLIATLGIVSTSPAAVRSAQRLAPLIEGTTAREMSRVG
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]