BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is echA12 [H]

Identifier: 169627933

GI number: 169627933

Start: 829922

End: 830734

Strand: Direct

Name: echA12 [H]

Synonym: MAB_0834

Alternate gene names: 169627933

Gene position: 829922-830734 (Clockwise)

Preceding gene: 169627932

Following gene: 169627940

Centisome position: 16.38

GC content: 62.98

Gene sequence:

>813_bases
ATGATCCGTGTCGAGCCGCGGGAACACGGAATCCTGGTAGTCACCATTGATCGTCCGGAACGTCGCAATGCCTTGGACCC
GCAGACGGTGGCCGAGCTGCATGCGGTGATCGAGAGCATCGATGGAGATCCCAAGGTTCGTGTGGTGGTGCTCACCGCCG
TCGGCGCATCCTTTTGTGCGGGAGCCGATCTGAAGTCGCTACCGAGTGATTTCACCAGCGCCGGGGTAACCTCCACAGCC
GCACTGCTTGCCGCCACCGACTCCGAGCTGGTGCGTACGCTCGCCGCGCAGGAGCTGATGGCGTCTCTATTCGAGCGGAT
ACACCGGATGCGGCAGCCGGTCATTGCGGCCGTCAACGGGCACGCGATAGGCGGCGGCTTTGCCCTGGCCCTCGCCTGTG
ATATCCGTTTTGCCAGTCCCGCAGCATCTTTCGGCGCAGTGTTTATTCGTCATGGGGTTTCCAACTGCGATATGGGCACC
AGTTATCACTTGCCGCGACTGGTGGGTGCCGCCCGTGCTGCCGAGCTGATGTTGACGGGCCGTGTGTTTGACGCGCAGGA
GGCATACCGCATGGGTCTGGTCTTCGACGTGGTGCCCGCCGAGGAACTTCTGGATGCGGCGCTGTCGAAGGCCCGGGAGA
TCGCCGTTCACTCGCCGCTGGCCGTCTGGATGACCAAGGAGACGATGTGGCAGACGGTGGATGCGCCGAGCCTGCGGCAT
GCGCTGGATCTGGAGAATAGAACGCAGGTGATGTGCACTGCCACCGGAGAACTGCGTCGATCCTTTGACGCGTTCCGGGA
AGGGCGCGGCTAA

Upstream 100 bases:

>100_bases
TGCCTGACGAAATGGTAGGCACCGCACTCCTTCTGACCTCTGATGCGGGCAGTTACATCACCGGCCAGACCATCATCGCC
GATGGCGGGCTGGTGGCCCG

Downstream 100 bases:

>100_bases
CTCTCGTCCGGAGGCCCGGCCAAGATCGCCGTCACCATGTCGATCAGTTGTGCGTTCATCTGCCCATCGTCCAGATAGGT
TCCGCTCAGCGACATCAGGC

Product: putative enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MIRVEPREHGILVVTIDRPERRNALDPQTVAELHAVIESIDGDPKVRVVVLTAVGASFCAGADLKSLPSDFTSAGVTSTA
ALLAATDSELVRTLAAQELMASLFERIHRMRQPVIAAVNGHAIGGGFALALACDIRFASPAASFGAVFIRHGVSNCDMGT
SYHLPRLVGAARAAELMLTGRVFDAQEAYRMGLVFDVVPAEELLDAALSKAREIAVHSPLAVWMTKETMWQTVDAPSLRH
ALDLENRTQVMCTATGELRRSFDAFREGRG

Sequences:

>Translated_270_residues
MIRVEPREHGILVVTIDRPERRNALDPQTVAELHAVIESIDGDPKVRVVVLTAVGASFCAGADLKSLPSDFTSAGVTSTA
ALLAATDSELVRTLAAQELMASLFERIHRMRQPVIAAVNGHAIGGGFALALACDIRFASPAASFGAVFIRHGVSNCDMGT
SYHLPRLVGAARAAELMLTGRVFDAQEAYRMGLVFDVVPAEELLDAALSKAREIAVHSPLAVWMTKETMWQTVDAPSLRH
ALDLENRTQVMCTATGELRRSFDAFREGRG
>Mature_270_residues
MIRVEPREHGILVVTIDRPERRNALDPQTVAELHAVIESIDGDPKVRVVVLTAVGASFCAGADLKSLPSDFTSAGVTSTA
ALLAATDSELVRTLAAQELMASLFERIHRMRQPVIAAVNGHAIGGGFALALACDIRFASPAASFGAVFIRHGVSNCDMGT
SYHLPRLVGAARAAELMLTGRVFDAQEAYRMGLVFDVVPAEELLDAALSKAREIAVHSPLAVWMTKETMWQTVDAPSLRH
ALDLENRTQVMCTATGELRRSFDAFREGRG

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=267, Percent_Identity=31.0861423220974, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI70995211, Length=270, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI4502327, Length=271, Percent_Identity=30.6273062730627, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI221136756, Length=204, Percent_Identity=26.9607843137255, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221136753, Length=204, Percent_Identity=26.9607843137255, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221307494, Length=204, Percent_Identity=26.9607843137255, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI45643119, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260275230, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=27.027027027027, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI25453481, Length=220, Percent_Identity=25.9090909090909, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538573, Length=220, Percent_Identity=25.9090909090909, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4757966, Length=217, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538581, Length=217, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI31542718, Length=226, Percent_Identity=29.2035398230088, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4757968, Length=224, Percent_Identity=25, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI49355787, Length=224, Percent_Identity=25, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI116235487, Length=196, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI20127408, Length=199, Percent_Identity=28.1407035175879, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=28.8557213930348, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=31.9391634980989, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=231, Percent_Identity=35.9307359307359, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788597, Length=270, Percent_Identity=30.7407407407407, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=31.3953488372093, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788682, Length=211, Percent_Identity=36.0189573459716, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790281, Length=206, Percent_Identity=32.0388349514563, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=25.3787878787879, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=271, Percent_Identity=32.4723247232472, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=34.6666666666667, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=32.183908045977, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=232, Percent_Identity=29.3103448275862, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=30.3030303030303, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=30, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=228, Percent_Identity=32.0175438596491, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=200, Percent_Identity=30.5, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=221, Percent_Identity=28.9592760180996, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=268, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=268, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=33.852140077821, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=33.852140077821, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24653139, Length=229, Percent_Identity=37.9912663755458, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21357171, Length=234, Percent_Identity=29.9145299145299, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=34.3478260869565, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24650670, Length=221, Percent_Identity=32.1266968325792, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654903, Length=210, Percent_Identity=28.0952380952381, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922422, Length=273, Percent_Identity=24.5421245421245, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921000, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583077, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583079, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921018, Length=223, Percent_Identity=29.1479820627803, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29077; Mature: 29077

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVEPREHGILVVTIDRPERRNALDPQTVAELHAVIESIDGDPKVRVVVLTAVGASFCA
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHC
GADLKSLPSDFTSAGVTSTAALLAATDSELVRTLAAQELMASLFERIHRMRQPVIAAVNG
CCCHHHCCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
HAIGGGFALALACDIRFASPAASFGAVFIRHGVSNCDMGTSYHLPRLVGAARAAELMLTG
CEECCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEEC
RVFDAQEAYRMGLVFDVVPAEELLDAALSKAREIAVHSPLAVWMTKETMWQTVDAPSLRH
CEECHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCHHHH
ALDLENRTQVMCTATGELRRSFDAFREGRG
HHCCCCCCEEEEEECHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRVEPREHGILVVTIDRPERRNALDPQTVAELHAVIESIDGDPKVRVVVLTAVGASFCA
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHC
GADLKSLPSDFTSAGVTSTAALLAATDSELVRTLAAQELMASLFERIHRMRQPVIAAVNG
CCCHHHCCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
HAIGGGFALALACDIRFASPAASFGAVFIRHGVSNCDMGTSYHLPRLVGAARAAELMLTG
CEECCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEEC
RVFDAQEAYRMGLVFDVVPAEELLDAALSKAREIAVHSPLAVWMTKETMWQTVDAPSLRH
CEECHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCHHHH
ALDLENRTQVMCTATGELRRSFDAFREGRG
HHCCCCCCEEEEEECHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]