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The map label for this gene is purF [H]

Identifier: 169627814

GI number: 169627814

Start: 715278

End: 716792

Strand: Direct

Name: purF [H]

Synonym: MAB_0713

Alternate gene names: 169627814

Gene position: 715278-716792 (Clockwise)

Preceding gene: 169627812

Following gene: 169627818

Centisome position: 14.12

GC content: 65.74

Gene sequence:

>1515_bases
GTGACCGCACAGCCGATCGAGCTGGAAAACGACCCACGCGAAGAATGCGGCGTATTCGGAGTCTGGGCGCCGGGTGAAGA
GGTGGCAAAACTCACCTACTACGGCCTGTATGCGCTGCAGCACCGTGGTCAGGAGGCTGCCGGTATCGCTGTGGCCGACG
GCTCGCAGATCGTGGTGTTCAAGGATCTCGGCCTGGTCAGTCAGGTTTTCGACGAGCAGACCCTCGCCGCTATGCGTGGC
CACGTGGCAGTGGGGCACTGCCGGTACTCCACCACCGGGTCTGTCACCTGGGAGAACGCTCAGCCGGTCTTCCGCACCAC
CGCGGCGGGCACCGGAGTCGCCCTGGGACACAACGGAAACCTGGTCAATACCGCCGAGCTGACGGAGCGTGCGCGCGATG
CCGGTCTGATCAATTCGAAGACGCCCGGCATGGCCACCACCGACTCCGACATCATGGGTGCGTTGTTGGCGCACGGTGCC
GCCGACGCCACCCTGGAGCAGGCGGCCATGACGCTGCTGCCCACCGTGCGTGGCGCCTTCTGCTTGGTTTTCGCGGACGA
GAACACGCTGTATGCGGCGCGTGATCCGCACGGAGTCCGCCCGCTGTGCCTGGGACGTCTGGACACCGGCTGGGTGGTCG
CGTCAGAGACCGCCGCCCTGGACATCGTGGGCGCCTCCTTCGTCCGGGATATCGAGCCCGGTGAATTGCTCGCCATCGAC
GCCGATGGCGTGCGCTCCAGTCGGTTCGCCAATGCCACACCCAAGACCTGCGTCTTCGAATACGTGTATCTGGCCCGCCC
CGACAGCATGCTCGACGGCCGTTCCGTGCACTCGACCCGTGTGGAGATCGGGCGCCGCTTGGCTGCCGAACACTCTGTGG
ATGCCGACCTGGTGATCGGAGTTCCCGAGTCGGGCACCCCGGCGGCCGTCGGCTACGCCCAGGGCTCGGGCATCCCCTAC
GGGCAGGGGCTGATGAAGAACGCCTATGTGGGCCGCACCTTCATTCAGCCGTCACAGACCATCCGCCAGCTCGGTATTCG
TCTGAAGCTCAACCCCCTTCGCGAGGTCATTCGCGGTAAGCGCCTTGTGGTGGTGGACGACTCGATCGTTCGCGGCAACA
CGCAGCGGGCGCTGGTGCGGATGCTGCGCGAGGCGGGAGCCGCCGAGGTGCATGTGCGCATCGCATCGCCGCCGGTGCGC
TGGCCATGCTTCTACGGCATCGACTTCGCTTCCCCGGCCGAACTTATCGCCAACGCGGTGGAATCCGATGACGAGATGCT
CGACGCTGTCCGCACCGCGATCGGTGCGGACAGCCTGGGCTACATTTCCGCCGAGGGTATGGTCGCGGCCACCCAGGAAC
CGCGGTCGAGGTTGTGCTGCGCATGCTTCGACGGTAATTATCCGATCGAACTGCCCAAGGAGACCGCGCTCGGCAAGAAC
GTGGTCGAGCACATGCTTGCGGCCACTGCGCGGGAGACATCGAGCGAGAGTTCGGAATCCGTCCAGGCGCGCTAA

Upstream 100 bases:

>100_bases
CCGATGTGTTCGGGCCAGCCGATTCCCCGATCAGCAACCTTCCCCGTAAACTGGGGGAGTCACCCACCTCCACGCAGGAC
GCAGCCCAGGGAGCCACACC

Downstream 100 bases:

>100_bases
CGTCCTTGCCCGCGCGGCGGCGGGTACGCCTCGTCGACGATCACGTCTCGCAGCCCGGGAACGCCGGTCCTGATGAGTTC
GATGTGAGTGTCGATCATGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MTAQPIELENDPREECGVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQIVVFKDLGLVSQVFDEQTLAAMRG
HVAVGHCRYSTTGSVTWENAQPVFRTTAAGTGVALGHNGNLVNTAELTERARDAGLINSKTPGMATTDSDIMGALLAHGA
ADATLEQAAMTLLPTVRGAFCLVFADENTLYAARDPHGVRPLCLGRLDTGWVVASETAALDIVGASFVRDIEPGELLAID
ADGVRSSRFANATPKTCVFEYVYLARPDSMLDGRSVHSTRVEIGRRLAAEHSVDADLVIGVPESGTPAAVGYAQGSGIPY
GQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLVVVDDSIVRGNTQRALVRMLREAGAAEVHVRIASPPVR
WPCFYGIDFASPAELIANAVESDDEMLDAVRTAIGADSLGYISAEGMVAATQEPRSRLCCACFDGNYPIELPKETALGKN
VVEHMLAATARETSSESSESVQAR

Sequences:

>Translated_504_residues
MTAQPIELENDPREECGVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQIVVFKDLGLVSQVFDEQTLAAMRG
HVAVGHCRYSTTGSVTWENAQPVFRTTAAGTGVALGHNGNLVNTAELTERARDAGLINSKTPGMATTDSDIMGALLAHGA
ADATLEQAAMTLLPTVRGAFCLVFADENTLYAARDPHGVRPLCLGRLDTGWVVASETAALDIVGASFVRDIEPGELLAID
ADGVRSSRFANATPKTCVFEYVYLARPDSMLDGRSVHSTRVEIGRRLAAEHSVDADLVIGVPESGTPAAVGYAQGSGIPY
GQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLVVVDDSIVRGNTQRALVRMLREAGAAEVHVRIASPPVR
WPCFYGIDFASPAELIANAVESDDEMLDAVRTAIGADSLGYISAEGMVAATQEPRSRLCCACFDGNYPIELPKETALGKN
VVEHMLAATARETSSESSESVQAR
>Mature_503_residues
TAQPIELENDPREECGVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQIVVFKDLGLVSQVFDEQTLAAMRGH
VAVGHCRYSTTGSVTWENAQPVFRTTAAGTGVALGHNGNLVNTAELTERARDAGLINSKTPGMATTDSDIMGALLAHGAA
DATLEQAAMTLLPTVRGAFCLVFADENTLYAARDPHGVRPLCLGRLDTGWVVASETAALDIVGASFVRDIEPGELLAIDA
DGVRSSRFANATPKTCVFEYVYLARPDSMLDGRSVHSTRVEIGRRLAAEHSVDADLVIGVPESGTPAAVGYAQGSGIPYG
QGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLVVVDDSIVRGNTQRALVRMLREAGAAEVHVRIASPPVRW
PCFYGIDFASPAELIANAVESDDEMLDAVRTAIGADSLGYISAEGMVAATQEPRSRLCCACFDGNYPIELPKETALGKNV
VEHMLAATARETSSESSESVQAR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=41.0404624277457, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1788651, Length=478, Percent_Identity=38.4937238493724, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1790167, Length=232, Percent_Identity=28.0172413793103, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=492, Percent_Identity=38.2113821138211, Blast_Score=311, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6323958, Length=484, Percent_Identity=34.504132231405, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24659598, Length=505, Percent_Identity=41.3861386138614, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24659604, Length=480, Percent_Identity=41.875, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI28573187, Length=509, Percent_Identity=40.8644400785855, Blast_Score=352, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53758; Mature: 53627

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQPIELENDPREECGVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQIVVF
CCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEE
KDLGLVSQVFDEQTLAAMRGHVAVGHCRYSTTGSVTWENAQPVFRTTAAGTGVALGHNGN
ECCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEECCCCCCEEEECCCCCEEECCCCC
LVNTAELTERARDAGLINSKTPGMATTDSDIMGALLAHGAADATLEQAAMTLLPTVRGAF
EEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
CLVFADENTLYAARDPHGVRPLCLGRLDTGWVVASETAALDIVGASFVRDIEPGELLAID
EEEEECCCEEEEECCCCCCCCCEECCCCCCEEEECCCCEEEEECHHHHHCCCCCCEEEEE
ADGVRSSRFANATPKTCVFEYVYLARPDSMLDGRSVHSTRVEIGRRLAAEHSVDADLVIG
CCCCCCCCCCCCCCCHHHEEEHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
VPESGTPAAVGYAQGSGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGK
CCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCEECCHHHHHHHCCCEEEEHHHHHHHCCC
RLVVVDDSIVRGNTQRALVRMLREAGAAEVHVRIASPPVRWPCFYGIDFASPAELIANAV
EEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHH
ESDDEMLDAVRTAIGADSLGYISAEGMVAATQEPRSRLCCACFDGNYPIELPKETALGKN
CCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHH
VVEHMLAATARETSSESSESVQAR
HHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TAQPIELENDPREECGVFGVWAPGEEVAKLTYYGLYALQHRGQEAAGIAVADGSQIVVF
CCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEE
KDLGLVSQVFDEQTLAAMRGHVAVGHCRYSTTGSVTWENAQPVFRTTAAGTGVALGHNGN
ECCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEECCCCCCEEEECCCCCEEECCCCC
LVNTAELTERARDAGLINSKTPGMATTDSDIMGALLAHGAADATLEQAAMTLLPTVRGAF
EEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
CLVFADENTLYAARDPHGVRPLCLGRLDTGWVVASETAALDIVGASFVRDIEPGELLAID
EEEEECCCEEEEECCCCCCCCCEECCCCCCEEEECCCCEEEEECHHHHHCCCCCCEEEEE
ADGVRSSRFANATPKTCVFEYVYLARPDSMLDGRSVHSTRVEIGRRLAAEHSVDADLVIG
CCCCCCCCCCCCCCCHHHEEEHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
VPESGTPAAVGYAQGSGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGK
CCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCEECCHHHHHHHCCCEEEEHHHHHHHCCC
RLVVVDDSIVRGNTQRALVRMLREAGAAEVHVRIASPPVRWPCFYGIDFASPAELIANAV
EEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHH
ESDDEMLDAVRTAIGADSLGYISAEGMVAATQEPRSRLCCACFDGNYPIELPKETALGKN
CCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHH
VVEHMLAATARETSSESSESVQAR
HHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]