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The map label for this gene is cocE [H]

Identifier: 169627605

GI number: 169627605

Start: 497906

End: 499552

Strand: Reverse

Name: cocE [H]

Synonym: MAB_0501c

Alternate gene names: 169627605

Gene position: 499552-497906 (Counterclockwise)

Preceding gene: 169627606

Following gene: 169627604

Centisome position: 9.86

GC content: 63.33

Gene sequence:

>1647_bases
ATGCTCGAAGAACTGGCTTTGACGTGGACCGGACGACTGTCCGCACCCGTCCTCGAGAAGCTACTCGACCTGCCCGAACC
ACTCACCACCGATATACAGACGCAGCGGGACATCACGGTGCGGATGCCCGACGGGGTGCGACTCTCCACCGACCACTTCC
GCCCACCCGGTCAGCACCCGCTGCCGGCCGTCATCTTTCGCACCCCATACGACAAGCGCGGCATGGTGAGCCAACTGTGG
GCAATGCTGTTGGCGCGTCAGGGGTTTCAGGTGATCGTGCAAGACACCCGAGGCCAGTACGGATCCCAGGGCAAATTCGA
CGCGTTTCGGCAGGAGCGGGCGGACGGCCTGGCCACGGCGGCATGGGTTCGCGAGCAGCCGTGGTGCGATGGCACCCTGG
CAACCGCCGGGCCTTCATACCTCGGGCACACCCAATGGGCGGTGGCGCCGTATATCGAGCCGCCCCTGGCCGCCATGTGC
CCCGCCATCACCACCAGTAACTTCAGAGAGCTCTTCTATCCGGGCGGGTCATTCAATCTGCACAACCTGCTGACGTGGTC
GTCGTCGATCGGTTCACCGGAAACCTCCCAGCTGACCCGTCTGCTGCGGGCCGGCGCAATCGCCAAGCGAGTACGCCGGG
CCATGGATCACCTACCCCTAGGCAATGCCGACAATATCGCCATCGGGAAGCCAGAACCTTTCTGGCGCACAGTAATCGAT
CACACCGAAGACGACCACTGGGACGAGATCAATCACACGCCGGCCCTCGCGACCCTCAACACACCGGTGAGCATGGTGAC
CGGCTGGTGGGACCTGCTTCTGGTCGGACAGTTACGCGATTACGAGGAACTGCAGAAGACGGACTGCCCGCGACGGATCA
CCATAGGCCCGTGGGACCACATGAAGTCCCTCAAGGCGGTGATCGGTGATAGCTTCTCGTGGCTTGCGGCCCACCTGCAC
GGTGACGCCTCGGCGACGCACCGGGCTCAAGTCCGTATCTATCTACAGAAAGCCGGTCAATGGCTGGACTTCGACCACTG
GCCTCCCCGGCAATCCACATCCACCGCGCTATACCTGCACGCGGATCGCGCATTGGATTGGGAAGCGCCACAGGGCGATA
GCTCTGCCGACAACTTTACCTACGACCCCAAGGACCCCACCCCGACGGTCGGCGGGCCACTGCTGGACACCCGGGCCGCC
GGCCAACGCGACAATTCCGCTATCGAGAAGCGGCCCGATGTCCTGGTGTTCACATCCCAGCCGTTGACCAGCGACCTGGA
CCTCATCGGCCCCGTCTCAGCCTCCATCTACGCGACCACCGACAGCGGCCAGGGCGATCTCTTCGTCAGACTCTGCGACG
TCGACCGTAAGGGACGGTCTCACAATGTCACGGACGGGATAATCAAATTAGACGGCGTCACGGACGCCCCGATTCAGGTG
ATCATGCACCCCACCGGCTACCGCTTCGCCCGTGGACACCGTCTGCGGTTACAGGTCAGCGGTGGCGGATTTCCGAATCA
CGCCCGCAACACCGGATCGGGCGAGCCACTGGCCACCGCCAGCCGAATCGTTCCGATCCACTTCGAGGTGCGCCACGACA
AGGAACACCCCTCCTCGGTGACACTTCCGGTGATGCACTCTTCATGA

Upstream 100 bases:

>100_bases
TCAGGGAATGCGATTATCCTGCAACCTATTACACAGTCATTGTCGCTGTGCGTCGCCGTCGGGCCGGTAGGTGCGCTCGC
CCCCCGGTAGATTGGGCGCC

Downstream 100 bases:

>100_bases
TCACGAAATACCTTACTCCCCTTGCACTTATCGGCACTCTCGTCAGCGCCTGCGGAGGCAGCGGCCCATCGGAAAGCCAT
CCGACCCTCATCCATGTCGG

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MLEELALTWTGRLSAPVLEKLLDLPEPLTTDIQTQRDITVRMPDGVRLSTDHFRPPGQHPLPAVIFRTPYDKRGMVSQLW
AMLLARQGFQVIVQDTRGQYGSQGKFDAFRQERADGLATAAWVREQPWCDGTLATAGPSYLGHTQWAVAPYIEPPLAAMC
PAITTSNFRELFYPGGSFNLHNLLTWSSSIGSPETSQLTRLLRAGAIAKRVRRAMDHLPLGNADNIAIGKPEPFWRTVID
HTEDDHWDEINHTPALATLNTPVSMVTGWWDLLLVGQLRDYEELQKTDCPRRITIGPWDHMKSLKAVIGDSFSWLAAHLH
GDASATHRAQVRIYLQKAGQWLDFDHWPPRQSTSTALYLHADRALDWEAPQGDSSADNFTYDPKDPTPTVGGPLLDTRAA
GQRDNSAIEKRPDVLVFTSQPLTSDLDLIGPVSASIYATTDSGQGDLFVRLCDVDRKGRSHNVTDGIIKLDGVTDAPIQV
IMHPTGYRFARGHRLRLQVSGGGFPNHARNTGSGEPLATASRIVPIHFEVRHDKEHPSSVTLPVMHSS

Sequences:

>Translated_548_residues
MLEELALTWTGRLSAPVLEKLLDLPEPLTTDIQTQRDITVRMPDGVRLSTDHFRPPGQHPLPAVIFRTPYDKRGMVSQLW
AMLLARQGFQVIVQDTRGQYGSQGKFDAFRQERADGLATAAWVREQPWCDGTLATAGPSYLGHTQWAVAPYIEPPLAAMC
PAITTSNFRELFYPGGSFNLHNLLTWSSSIGSPETSQLTRLLRAGAIAKRVRRAMDHLPLGNADNIAIGKPEPFWRTVID
HTEDDHWDEINHTPALATLNTPVSMVTGWWDLLLVGQLRDYEELQKTDCPRRITIGPWDHMKSLKAVIGDSFSWLAAHLH
GDASATHRAQVRIYLQKAGQWLDFDHWPPRQSTSTALYLHADRALDWEAPQGDSSADNFTYDPKDPTPTVGGPLLDTRAA
GQRDNSAIEKRPDVLVFTSQPLTSDLDLIGPVSASIYATTDSGQGDLFVRLCDVDRKGRSHNVTDGIIKLDGVTDAPIQV
IMHPTGYRFARGHRLRLQVSGGGFPNHARNTGSGEPLATASRIVPIHFEVRHDKEHPSSVTLPVMHSS
>Mature_548_residues
MLEELALTWTGRLSAPVLEKLLDLPEPLTTDIQTQRDITVRMPDGVRLSTDHFRPPGQHPLPAVIFRTPYDKRGMVSQLW
AMLLARQGFQVIVQDTRGQYGSQGKFDAFRQERADGLATAAWVREQPWCDGTLATAGPSYLGHTQWAVAPYIEPPLAAMC
PAITTSNFRELFYPGGSFNLHNLLTWSSSIGSPETSQLTRLLRAGAIAKRVRRAMDHLPLGNADNIAIGKPEPFWRTVID
HTEDDHWDEINHTPALATLNTPVSMVTGWWDLLLVGQLRDYEELQKTDCPRRITIGPWDHMKSLKAVIGDSFSWLAAHLH
GDASATHRAQVRIYLQKAGQWLDFDHWPPRQSTSTALYLHADRALDWEAPQGDSSADNFTYDPKDPTPTVGGPLLDTRAA
GQRDNSAIEKRPDVLVFTSQPLTSDLDLIGPVSASIYATTDSGQGDLFVRLCDVDRKGRSHNVTDGIIKLDGVTDAPIQV
IMHPTGYRFARGHRLRLQVSGGGFPNHARNTGSGEPLATASRIVPIHFEVRHDKEHPSSVTLPVMHSS

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 60794; Mature: 60794

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEELALTWTGRLSAPVLEKLLDLPEPLTTDIQTQRDITVRMPDGVRLSTDHFRPPGQHP
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCC
LPAVIFRTPYDKRGMVSQLWAMLLARQGFQVIVQDTRGQYGSQGKFDAFRQERADGLATA
CCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
AWVREQPWCDGTLATAGPSYLGHTQWAVAPYIEPPLAAMCPAITTSNFRELFYPGGSFNL
HHHHCCCCCCCEECCCCCCCCCCCCEEECCCCCCCHHHHCCCCCCCCCHHEECCCCCCCH
HNLLTWSSSIGSPETSQLTRLLRAGAIAKRVRRAMDHLPLGNADNIAIGKPEPFWRTVID
HHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHC
HTEDDHWDEINHTPALATLNTPVSMVTGWWDLLLVGQLRDYEELQKTDCPRRITIGPWDH
CCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCHHH
MKSLKAVIGDSFSWLAAHLHGDASATHRAQVRIYLQKAGQWLDFDHWPPRQSTSTALYLH
HHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEE
ADRALDWEAPQGDSSADNFTYDPKDPTPTVGGPLLDTRAAGQRDNSAIEKRPDVLVFTSQ
ECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECC
PLTSDLDLIGPVSASIYATTDSGQGDLFVRLCDVDRKGRSHNVTDGIIKLDGVTDAPIQV
CCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEE
IMHPTGYRFARGHRLRLQVSGGGFPNHARNTGSGEPLATASRIVPIHFEVRHDKEHPSSV
EECCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCE
TLPVMHSS
EEEEEECC
>Mature Secondary Structure
MLEELALTWTGRLSAPVLEKLLDLPEPLTTDIQTQRDITVRMPDGVRLSTDHFRPPGQHP
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCC
LPAVIFRTPYDKRGMVSQLWAMLLARQGFQVIVQDTRGQYGSQGKFDAFRQERADGLATA
CCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
AWVREQPWCDGTLATAGPSYLGHTQWAVAPYIEPPLAAMCPAITTSNFRELFYPGGSFNL
HHHHCCCCCCCEECCCCCCCCCCCCEEECCCCCCCHHHHCCCCCCCCCHHEECCCCCCCH
HNLLTWSSSIGSPETSQLTRLLRAGAIAKRVRRAMDHLPLGNADNIAIGKPEPFWRTVID
HHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHC
HTEDDHWDEINHTPALATLNTPVSMVTGWWDLLLVGQLRDYEELQKTDCPRRITIGPWDH
CCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCHHH
MKSLKAVIGDSFSWLAAHLHGDASATHRAQVRIYLQKAGQWLDFDHWPPRQSTSTALYLH
HHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEE
ADRALDWEAPQGDSSADNFTYDPKDPTPTVGGPLLDTRAAGQRDNSAIEKRPDVLVFTSQ
ECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECC
PLTSDLDLIGPVSASIYATTDSGQGDLFVRLCDVDRKGRSHNVTDGIIKLDGVTDAPIQV
CCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEE
IMHPTGYRFARGHRLRLQVSGGGFPNHARNTGSGEPLATASRIVPIHFEVRHDKEHPSSV
EECCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCE
TLPVMHSS
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]