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The map label for this gene is yfhM [H]

Identifier: 169627526

GI number: 169627526

Start: 421261

End: 422223

Strand: Reverse

Name: yfhM [H]

Synonym: MAB_0422c

Alternate gene names: 169627526

Gene position: 422223-421261 (Counterclockwise)

Preceding gene: 169627527

Following gene: 169627521

Centisome position: 8.33

GC content: 66.15

Gene sequence:

>963_bases
ATGCTGTTCGACCAGACAGCTCGTCACCCCGATCCATCCGTGGTCCGTATCAGTGGCCCGTGGCGTCACTTCGACGTACA
TGCCAACGGGATTCGGTTCCACGTGGTGGAAGCCGACGCCCAGGACGCACCGACCGCACCCGTTACAAGCCGTCCACTGG
TGCTGCTGCTCCACGGGTTTGGCTCCTTCTGGTGGTCATGGCGCCATCAACTCGGCGCTCTTCCCGGTGCCCGGGTGGTG
GCTGTCGACCTGCGTGGTTACGGCGGCAGCGACAAGCCGCCACGTGGATACGACGGATGGACCCTCGCCGGCGACACCGC
CGGATTGATTCGGGCTCTGGGCCACACCTCGGCCACGCTGGTCGGGCATGCCGAAGGCGGACTGGTCTGCTGGGCCACTG
CTAACCTGCATCCCCGCCTGGTCAATGCCATTGCGGTGATCAGCTCACCGCATCCGATCGCCCTGCGCACCTCGACGTTC
CGCGGAACCGGACAAGGCGGGGCACTGCTGCCCTCACTGATGCGTTACCAAGTGCCGATCCTGCCCGAGCGCACCCTAAC
CCGTCATCGCGCCGCCGAGCTGGAGCGTCTGGTGCGCTCGCGTGCGGGCACCCGCTGGACCAGCACCGACGACTTTGCGC
AGAGCATGAAGCACCTGCGCACCGCAATCTGCATTCCCGGGGTGGCCCACTGTGCGCTGGAATATCAGCGCTGGGCGGTG
CGCAGCCAATTGCGCAGCGAAGGTTGGCGTTTCATGCGGTCCATGAATCGCGAGCTGTCGGTTCCGGTGCTGCACATGCG
CGGCGAGTCGGATCCGTATGTGCTCGCCGACCCGGTGCATCGCTCACTGCCGTACGCGCCCAACGGGCAGTTCGTCGCGC
TACGCGGGGTGGGCCACTACGTCCACGAGGAAGACCCCGCCGCTGTGAACGAACATCTACGGCGCTTTACCGGGCACCGG
TGA

Upstream 100 bases:

>100_bases
TCAGCCGCGAGGATGAGGCACCCTCGGACACCAAAGCCATCAAGCCCGTAGACCCGTCGGGCTGGTAGCCGTCTGACGTC
GGCCCGGTACCGACACCTCC

Downstream 100 bases:

>100_bases
CTGCGGGCCGTTGACGGGCTGCTCGGTCGGCCTATTGGCTTCGGCCGCGGAGACGCAACTGCCGGTGGCCACAGGGTCGG
CTAGGTCCGCCTTGGCGAGC

Product: epoxide hydrolase EphE

Products: glycol

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLFDQTARHPDPSVVRISGPWRHFDVHANGIRFHVVEADAQDAPTAPVTSRPLVLLLHGFGSFWWSWRHQLGALPGARVV
AVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHTSATLVGHAEGGLVCWATANLHPRLVNAIAVISSPHPIALRTSTF
RGTGQGGALLPSLMRYQVPILPERTLTRHRAAELERLVRSRAGTRWTSTDDFAQSMKHLRTAICIPGVAHCALEYQRWAV
RSQLRSEGWRFMRSMNRELSVPVLHMRGESDPYVLADPVHRSLPYAPNGQFVALRGVGHYVHEEDPAAVNEHLRRFTGHR

Sequences:

>Translated_320_residues
MLFDQTARHPDPSVVRISGPWRHFDVHANGIRFHVVEADAQDAPTAPVTSRPLVLLLHGFGSFWWSWRHQLGALPGARVV
AVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHTSATLVGHAEGGLVCWATANLHPRLVNAIAVISSPHPIALRTSTF
RGTGQGGALLPSLMRYQVPILPERTLTRHRAAELERLVRSRAGTRWTSTDDFAQSMKHLRTAICIPGVAHCALEYQRWAV
RSQLRSEGWRFMRSMNRELSVPVLHMRGESDPYVLADPVHRSLPYAPNGQFVALRGVGHYVHEEDPAAVNEHLRRFTGHR
>Mature_320_residues
MLFDQTARHPDPSVVRISGPWRHFDVHANGIRFHVVEADAQDAPTAPVTSRPLVLLLHGFGSFWWSWRHQLGALPGARVV
AVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHTSATLVGHAEGGLVCWATANLHPRLVNAIAVISSPHPIALRTSTF
RGTGQGGALLPSLMRYQVPILPERTLTRHRAAELERLVRSRAGTRWTSTDDFAQSMKHLRTAICIPGVAHCALEYQRWAV
RSQLRSEGWRFMRSMNRELSVPVLHMRGESDPYVLADPVHRSLPYAPNGQFVALRGVGHYVHEEDPAAVNEHLRRFTGHR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=318, Percent_Identity=28.6163522012579, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI218777840, Length=320, Percent_Identity=27.8125, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI13376164, Length=320, Percent_Identity=27.8125, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI27597073, Length=311, Percent_Identity=24.1157556270096, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562298, Length=319, Percent_Identity=26.3322884012539, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554012, Length=320, Percent_Identity=25.625, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.3.2.3

Molecular weight: Translated: 35491; Mature: 35491

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFDQTARHPDPSVVRISGPWRHFDVHANGIRFHVVEADAQDAPTAPVTSRPLVLLLHGF
CCCCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHH
GSFWWSWRHQLGALPGARVVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHTSATL
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEE
VGHAEGGLVCWATANLHPRLVNAIAVISSPHPIALRTSTFRGTGQGGALLPSLMRYQVPI
EEECCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCC
LPERTLTRHRAAELERLVRSRAGTRWTSTDDFAQSMKHLRTAICIPGVAHCALEYQRWAV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RSQLRSEGWRFMRSMNRELSVPVLHMRGESDPYVLADPVHRSLPYAPNGQFVALRGVGHY
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCHHHCCCCCCCCCEEEEECCCCH
VHEEDPAAVNEHLRRFTGHR
HCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MLFDQTARHPDPSVVRISGPWRHFDVHANGIRFHVVEADAQDAPTAPVTSRPLVLLLHGF
CCCCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHH
GSFWWSWRHQLGALPGARVVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHTSATL
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEE
VGHAEGGLVCWATANLHPRLVNAIAVISSPHPIALRTSTFRGTGQGGALLPSLMRYQVPI
EEECCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCC
LPERTLTRHRAAELERLVRSRAGTRWTSTDDFAQSMKHLRTAICIPGVAHCALEYQRWAV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RSQLRSEGWRFMRSMNRELSVPVLHMRGESDPYVLADPVHRSLPYAPNGQFVALRGVGHY
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCHHHCCCCCCCCCEEEEECCCCH
VHEEDPAAVNEHLRRFTGHR
HCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; epoxide

Specific reaction: H2O + an epoxide = a glycol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]