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The map label for this gene is dhaK [H]

Identifier: 169627299

GI number: 169627299

Start: 203672

End: 205336

Strand: Direct

Name: dhaK [H]

Synonym: MAB_0194

Alternate gene names: 169627299

Gene position: 203672-205336 (Clockwise)

Preceding gene: 169627292

Following gene: 169627302

Centisome position: 4.02

GC content: 70.87

Gene sequence:

>1665_bases
ATGGTCCCGTTGCGGAACTTCCTGAACACCACGGAAAGCTTCATCCCCGACGCGCTGCGGGGACTGGTCGCGGCCACCGC
CGACCTGTGGTGGGATCGTGAGCACGGATATCTGATCCGGGATTCGCCATTGCGGCCCGGCCAGGTGGCCATCGTGTCGG
GCGGCGGCTCGGGGCACGAGCCCCTGCACGCCGGATTCATCGGTCGGGGCATGCTGACCGCCGCATGTCCCGGACTGATT
TTCACCTCGCCGAACGCCTTGCAGATCGCAGGCGCCACTGTCGCGGCCGATGCCGGTGGTGGCGTGCTGCACGTGGTGAA
GAACTACACCGGTGACGTGATGAACTTCTCCATCGCGCGCCAGATCGCCGGCGAATCCTCGGTGCGTACCGAGGTGGTGT
TGGTCGATGACGACGTGGCCACCGAGGTGGCGGACGCCGCCAAACCGGGCCGCCGTGGCACGGCCGCCACCGTGGTGGTC
GAAAAGCTGTGTGGCGCCGCCGCCGAACGTGGTGACCAGCTGGCCGCGGTGGCCGCGCTGGGACGTCGGGTCGCGGCGTC
CGCACGCAGCATGGCGGTCGCGCTGTCGCCGTGCACGGTCCCCGGTGCGCCGGTGCCGTCATTCGATCTGCCCACGGGCG
AGATGGAAATTGGGGTCGGGATCCACGGCGAGCGCGGTGTCGATCGTGCGCCGGTCGCCCCCGCCGCAGAGATCGTGGCC
GCGCTGCTGGCTCGCATCCTGCCGGCGGCGCAGGTGCGGTCCGGGGATGAGGTGATCGTTGTCGTCAACGGGCTCGGGGC
CACCCACTCGCTGGAGCTCAATCTCCTCTTCGGCGAGGTGGCCGCGCAGCTGGCGGCCGCAGGAATCGCCGTGGGCCGCA
GCCTTGTCGGCAGCTTCGTGACCGCGCTGGACATGGCGGGTGCTTCGATCACCGTGGTGCGCGCAGACCCGGAGATGTTC
GAGCTGTGGGACGCGCCCACCAGCGCCCCGGGCTGGCCGGCGGTGACGGGTCCGCCCGTCGGCAGGCTCATCGACGGGAC
AATCGTCGAGCCCACGGACAGGGCCGACCGCGGTGGGGAAAATCGTTGGCTCTCCGCGTTTGTGGAGCGCGTGCGGGCAT
CCGTGGGCATGCTCACCGACTTGGACCGGCGCGCCGGAGACGGTGATTTCGGGACGAATATGGCCGCCGCGCTGCGGCAC
TACGAGCTGCCGCTGCGCGGAACCGATGCCGATGTGCTGCTTGCCCTGTCGACCTCGTATTTCGTGCGCGCGGGCGGCAC
CTCCGGTGCGGTATTCGGCACCTTCTTCCGTGCGTTGTACGGCGGGCTCGGCAGCGAGCCCTGGTCTACCGAGCGGGTGG
CCCATGCCGTGCGGCACGGTCTGGATCGGATCCAGGCGCTGGGCGGCGCTCGGGTCGGCGACAAGACCGTGGTCGACGCC
GTGTCGCCGGCCGCGGACGCCCTCGATGCCGCTGTGCGACAGGGCATCCCACTGGCCCTGGCCCTGCGGTCCGCCGCCGA
TGCGGCCGCGGCGGGTGTCCAGGCCACCAGGGATGCGATAGCCGCGCGCGGCCGGGCCAGCTACGTGGGTGAGGCGGCCC
GCGGCGTCGAAGATCCCGGTGCCCTGGTCATGTCGTGGTTCTTCGAGGCGGCCGCCGGCCGCTGA

Upstream 100 bases:

>100_bases
GACCTGTCACGCGATCCGAATCCGGGCGTTGCCGACCACGCCAAGGATGACGAGTAGGTCGCCATAGCTTTTGGCCGCAG
GTGAGCCATACGGTTGCCGT

Downstream 100 bases:

>100_bases
CGTCTACACGCGCTGCCCGACAAATGTCTCTATTTCGCGCACCATCAGCTCCGGTTGGTCCTCGCTGACAAACGTGTAGC
TGTCGGGCACCTCGACGAGC

Product: dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MVPLRNFLNTTESFIPDALRGLVAATADLWWDREHGYLIRDSPLRPGQVAIVSGGGSGHEPLHAGFIGRGMLTAACPGLI
FTSPNALQIAGATVAADAGGGVLHVVKNYTGDVMNFSIARQIAGESSVRTEVVLVDDDVATEVADAAKPGRRGTAATVVV
EKLCGAAAERGDQLAAVAALGRRVAASARSMAVALSPCTVPGAPVPSFDLPTGEMEIGVGIHGERGVDRAPVAPAAEIVA
ALLARILPAAQVRSGDEVIVVVNGLGATHSLELNLLFGEVAAQLAAAGIAVGRSLVGSFVTALDMAGASITVVRADPEMF
ELWDAPTSAPGWPAVTGPPVGRLIDGTIVEPTDRADRGGENRWLSAFVERVRASVGMLTDLDRRAGDGDFGTNMAAALRH
YELPLRGTDADVLLALSTSYFVRAGGTSGAVFGTFFRALYGGLGSEPWSTERVAHAVRHGLDRIQALGGARVGDKTVVDA
VSPAADALDAAVRQGIPLALALRSAADAAAAGVQATRDAIAARGRASYVGEAARGVEDPGALVMSWFFEAAAGR

Sequences:

>Translated_554_residues
MVPLRNFLNTTESFIPDALRGLVAATADLWWDREHGYLIRDSPLRPGQVAIVSGGGSGHEPLHAGFIGRGMLTAACPGLI
FTSPNALQIAGATVAADAGGGVLHVVKNYTGDVMNFSIARQIAGESSVRTEVVLVDDDVATEVADAAKPGRRGTAATVVV
EKLCGAAAERGDQLAAVAALGRRVAASARSMAVALSPCTVPGAPVPSFDLPTGEMEIGVGIHGERGVDRAPVAPAAEIVA
ALLARILPAAQVRSGDEVIVVVNGLGATHSLELNLLFGEVAAQLAAAGIAVGRSLVGSFVTALDMAGASITVVRADPEMF
ELWDAPTSAPGWPAVTGPPVGRLIDGTIVEPTDRADRGGENRWLSAFVERVRASVGMLTDLDRRAGDGDFGTNMAAALRH
YELPLRGTDADVLLALSTSYFVRAGGTSGAVFGTFFRALYGGLGSEPWSTERVAHAVRHGLDRIQALGGARVGDKTVVDA
VSPAADALDAAVRQGIPLALALRSAADAAAAGVQATRDAIAARGRASYVGEAARGVEDPGALVMSWFFEAAAGR
>Mature_554_residues
MVPLRNFLNTTESFIPDALRGLVAATADLWWDREHGYLIRDSPLRPGQVAIVSGGGSGHEPLHAGFIGRGMLTAACPGLI
FTSPNALQIAGATVAADAGGGVLHVVKNYTGDVMNFSIARQIAGESSVRTEVVLVDDDVATEVADAAKPGRRGTAATVVV
EKLCGAAAERGDQLAAVAALGRRVAASARSMAVALSPCTVPGAPVPSFDLPTGEMEIGVGIHGERGVDRAPVAPAAEIVA
ALLARILPAAQVRSGDEVIVVVNGLGATHSLELNLLFGEVAAQLAAAGIAVGRSLVGSFVTALDMAGASITVVRADPEMF
ELWDAPTSAPGWPAVTGPPVGRLIDGTIVEPTDRADRGGENRWLSAFVERVRASVGMLTDLDRRAGDGDFGTNMAAALRH
YELPLRGTDADVLLALSTSYFVRAGGTSGAVFGTFFRALYGGLGSEPWSTERVAHAVRHGLDRIQALGGARVGDKTVVDA
VSPAADALDAAVRQGIPLALALRSAADAAAAGVQATRDAIAARGRASYVGEAARGVEDPGALVMSWFFEAAAGR

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=563, Percent_Identity=37.1225577264654, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI87081857, Length=353, Percent_Identity=42.2096317280453, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1787449, Length=196, Percent_Identity=32.1428571428571, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17565018, Length=577, Percent_Identity=33.1022530329289, Blast_Score=268, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321055, Length=547, Percent_Identity=33.8208409506399, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6323570, Length=581, Percent_Identity=31.1531841652324, Blast_Score=221, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 56817; Mature: 56817

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPLRNFLNTTESFIPDALRGLVAATADLWWDREHGYLIRDSPLRPGQVAIVSGGGSGHE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEECCCCCCC
PLHAGFIGRGMLTAACPGLIFTSPNALQIAGATVAADAGGGVLHVVKNYTGDVMNFSIAR
CCHHCHHHCCHHHHHCCCEEEECCCCEEEECCEEEECCCCCEEEEEHHCCCCHHHHHHHH
QIAGESSVRTEVVLVDDDVATEVADAAKPGRRGTAATVVVEKLCGAAAERGDQLAAVAAL
HHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
GRRVAASARSMAVALSPCTVPGAPVPSFDLPTGEMEIGVGIHGERGVDRAPVAPAAEIVA
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
ALLARILPAAQVRSGDEVIVVVNGLGATHSLELNLLFGEVAAQLAAAGIAVGRSLVGSFV
HHHHHHCCHHHCCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
TALDMAGASITVVRADPEMFELWDAPTSAPGWPAVTGPPVGRLIDGTIVEPTDRADRGGE
HHHHHCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCCC
NRWLSAFVERVRASVGMLTDLDRRAGDGDFGTNMAAALRHYELPLRGTDADVLLALSTSY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCE
FVRAGGTSGAVFGTFFRALYGGLGSEPWSTERVAHAVRHGLDRIQALGGARVGDKTVVDA
EEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VSPAADALDAAVRQGIPLALALRSAADAAAAGVQATRDAIAARGRASYVGEAARGVEDPG
CCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ALVMSWFFEAAAGR
HHHHHHHHHHHCCC
>Mature Secondary Structure
MVPLRNFLNTTESFIPDALRGLVAATADLWWDREHGYLIRDSPLRPGQVAIVSGGGSGHE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEECCCCCCC
PLHAGFIGRGMLTAACPGLIFTSPNALQIAGATVAADAGGGVLHVVKNYTGDVMNFSIAR
CCHHCHHHCCHHHHHCCCEEEECCCCEEEECCEEEECCCCCEEEEEHHCCCCHHHHHHHH
QIAGESSVRTEVVLVDDDVATEVADAAKPGRRGTAATVVVEKLCGAAAERGDQLAAVAAL
HHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
GRRVAASARSMAVALSPCTVPGAPVPSFDLPTGEMEIGVGIHGERGVDRAPVAPAAEIVA
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
ALLARILPAAQVRSGDEVIVVVNGLGATHSLELNLLFGEVAAQLAAAGIAVGRSLVGSFV
HHHHHHCCHHHCCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
TALDMAGASITVVRADPEMFELWDAPTSAPGWPAVTGPPVGRLIDGTIVEPTDRADRGGE
HHHHHCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCEECCCCCCCCCCC
NRWLSAFVERVRASVGMLTDLDRRAGDGDFGTNMAAALRHYELPLRGTDADVLLALSTSY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCE
FVRAGGTSGAVFGTFFRALYGGLGSEPWSTERVAHAVRHGLDRIQALGGARVGDKTVVDA
EEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VSPAADALDAAVRQGIPLALALRSAADAAAAGVQATRDAIAARGRASYVGEAARGVEDPG
CCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ALVMSWFFEAAAGR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]