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The map label for this gene is neo [H]

Identifier: 169627268

GI number: 169627268

Start: 157799

End: 158521

Strand: Reverse

Name: neo [H]

Synonym: MAB_0163c

Alternate gene names: 169627268

Gene position: 158521-157799 (Counterclockwise)

Preceding gene: 169627273

Following gene: 169627267

Centisome position: 3.13

GC content: 66.8

Gene sequence:

>723_bases
GTGACGATTCCGACCGAGCCGGTGACCATCCCCGATATCGTGGCACATCTCTCGGCGGGGCGCCCGGTGCTAGCGGTGTG
GCACAACCAAATTGGCGGCCATACTTTTCAGATCGGTAGCGGCGCCGACCGCGAGTTCGTGAAGGTGTCGGCGCCGCACC
CGGCCATCAACCTGCCTGCCGAGGCCGAAAGGCTCCGCTGGGCGCGCCGGTACACGACGGTGCCTACCGTGCTGTCCGTG
AACACCGAAGGCCCTCTGCATTGGCTGCACACCGCGGGCATCCCGGGCGACAGCGCGGTGGCGCCGCGATGGATCGAGAA
TCCGGAGGTGTCGGTGCGCGCCATCGCGTCTGGACTGCGCGCGCTGCACGATCGACTGCCTGTCGTTGACTGCCCCTACA
GCTGGTCGGTGGCCGCGCGGCTGGCCCGTATCCCCAATCCCGGTGGGCTGTCGGAGCACCCCGCGATCGATCGACTGGTG
GTGTGTCACGGGGATGCCTGCGCGCCCAATACCCTGCTCGACGGCAACGGGAACTGGTGCGGGCATGTGGATTTCGGTGA
CCTCGGGGTCGCTGACCGTTGGGCAGATCTCGCGGTGGCCAGCTGGTCACTGGAATGGAATTACGGCAAGGGCTGGAATG
ACGCCTTCTTCGCGGCGTACGGCGTGACCGAGGATCCCGAACGGATGGCCTACTACCGGCGGCTGTGGGATTCCGAGGAG
TGA

Upstream 100 bases:

>100_bases
ACGATGGCCGTGCGCCCGGACAGATCGTAGAGCGACTGGAAGTCAGTGAGGTTCACGCTCGGCACTCTATTCGGTTGACG
TCGCCGGGCAGACTGGAACG

Downstream 100 bases:

>100_bases
ACTTGCTGCCACCCCCCTGTGGCAAATTTCCGTAATCTAGATCGCATGCGTCCCACCACTCGCCGGTTCCTGCTGCTGGT
AGCCCTTGTCGCAACCCTCG

Product: phosphotransferase

Products: NA

Alternate protein names: APH(3')-II; APH(3')II; Kanamycin kinase, type II; Neomycin-kanamycin phosphotransferase type II [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTIPTEPVTIPDIVAHLSAGRPVLAVWHNQIGGHTFQIGSGADREFVKVSAPHPAINLPAEAERLRWARRYTTVPTVLSV
NTEGPLHWLHTAGIPGDSAVAPRWIENPEVSVRAIASGLRALHDRLPVVDCPYSWSVAARLARIPNPGGLSEHPAIDRLV
VCHGDACAPNTLLDGNGNWCGHVDFGDLGVADRWADLAVASWSLEWNYGKGWNDAFFAAYGVTEDPERMAYYRRLWDSEE

Sequences:

>Translated_240_residues
MTIPTEPVTIPDIVAHLSAGRPVLAVWHNQIGGHTFQIGSGADREFVKVSAPHPAINLPAEAERLRWARRYTTVPTVLSV
NTEGPLHWLHTAGIPGDSAVAPRWIENPEVSVRAIASGLRALHDRLPVVDCPYSWSVAARLARIPNPGGLSEHPAIDRLV
VCHGDACAPNTLLDGNGNWCGHVDFGDLGVADRWADLAVASWSLEWNYGKGWNDAFFAAYGVTEDPERMAYYRRLWDSEE
>Mature_239_residues
TIPTEPVTIPDIVAHLSAGRPVLAVWHNQIGGHTFQIGSGADREFVKVSAPHPAINLPAEAERLRWARRYTTVPTVLSVN
TEGPLHWLHTAGIPGDSAVAPRWIENPEVSVRAIASGLRALHDRLPVVDCPYSWSVAARLARIPNPGGLSEHPAIDRLVV
CHGDACAPNTLLDGNGNWCGHVDFGDLGVADRWADLAVASWSLEWNYGKGWNDAFFAAYGVTEDPERMAYYRRLWDSEE

Specific function: Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 26310; Mature: 26179

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPTEPVTIPDIVAHLSAGRPVLAVWHNQIGGHTFQIGSGADREFVKVSAPHPAINLPA
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCH
EAERLRWARRYTTVPTVLSVNTEGPLHWLHTAGIPGDSAVAPRWIENPEVSVRAIASGLR
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ALHDRLPVVDCPYSWSVAARLARIPNPGGLSEHPAIDRLVVCHGDACAPNTLLDGNGNWC
HHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCEE
GHVDFGDLGVADRWADLAVASWSLEWNYGKGWNDAFFAAYGVTEDPERMAYYRRLWDSEE
EECCCCCCCCCHHHHHEEEEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIPTEPVTIPDIVAHLSAGRPVLAVWHNQIGGHTFQIGSGADREFVKVSAPHPAINLPA
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCCH
EAERLRWARRYTTVPTVLSVNTEGPLHWLHTAGIPGDSAVAPRWIENPEVSVRAIASGLR
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ALHDRLPVVDCPYSWSVAARLARIPNPGGLSEHPAIDRLVVCHGDACAPNTLLDGNGNWC
HHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCCEE
GHVDFGDLGVADRWADLAVASWSLEWNYGKGWNDAFFAAYGVTEDPERMAYYRRLWDSEE
EECCCCCCCCCHHHHHEEEEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6295884; 3889831; 12628253 [H]