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The map label for this gene is gltD [H]

Identifier: 169627197

GI number: 169627197

Start: 92707

End: 94173

Strand: Direct

Name: gltD [H]

Synonym: MAB_0092

Alternate gene names: 169627197

Gene position: 92707-94173 (Clockwise)

Preceding gene: 169627196

Following gene: 169627198

Centisome position: 1.83

GC content: 65.85

Gene sequence:

>1467_bases
GTGGGTGACCCAAGCGGCTTTCTGAACCACACCACCCGCGAACTGCCCAAGCGGCGCCCCGTGCCGCTGCGGCTGCTCGA
CTGGAAAGAGGTCTATGAAGATTTCGAGTCTTCGAGACTTCAAATTCAGGCATCGCGCTGCATGGACTGCGGAATCCCGT
TCTGCCACAAGGGCTGCCCGCTGGGTAACCTGATTCCGGAGTGGAACGACATGGTGTACCGCGGGAACTGGCGCGAGGGC
ATCGAGCGGCTGCATGCGACCAACAACTTCCCCGAGTTCACCGGGCGGCTCTGCCCGGCGCCCTGTGAGGCATCGTGCGT
GCTGGGCATAAACCAGGACCCGGTGACCATCAAGCAGGTCGAGGTCGAGTTGATCGACAACGCCTTCGAGAACGACTGGG
TCAAGCCCATTCCGCCCGAGGTGAAGACCGGCAAGAAGGTAGCGGTCGTGGGCTCGGGCCCGGCGGGACTGGCTGCGGCA
CAGCAGCTCACCCGCGCCGGACACGACGTCACCGTCTACGAGCGCGCGGACCGGATCGGCGGGCTGCTGCGCTACGGCAT
CCCCGAGTTCAAAATGGAGAAGCGCCACATCGACCGGCGTCTGGAACAGATGCGGGCCGAAGGAACGGTTTTCGAGGCCG
GCGTGAATGTCGGAGTTGACATAACGGCGGATCACCTCCGTTCAAACTTCGACGCGGTGGTCCTCGCGGGCGGAGCGACC
GCCTGGCGTGACCTGCCGGTTCCCGGCCGCGAGCTCGAGGGCATCTACCAGGCCATGGAATACCTGCCCTGGGCCAATAA
GGTGCAGCTCGGCGATGATGTCGTGGACGCCGATGGCCAGCCGCCGATCACGGCGAAGGGCAAGCGCGTCATCATCATCG
GTGGTGGCGACACCGGCGCGGACTGCCTGGGCACCGCCCACCGGCAGGGTGCGGCGAGTGTTCAGCAGTTCGAGATCATG
CCCAAGCCGCCGGAGAGCCGTTCCGAGCGCGACCCGTGGCCCACCTACCCGACACTGTTCCGGGTGGCCTCCGCGCACGA
AGAGGGTGGCGAGCGGATCTACGCCGTCAACACCGAGCGGTTCCTCGGCGAGGACGGCAAGCTCACCGGTTTGCGTGCGC
ACGAGGTCGTCTTCAACGCCGGCAAGTTCGAGAAGGTGGAGGGCTCGGATTTCGAGCTGGAAGCCGACATCGTATTCCTG
GCAATGGGATTCGTCGGTCCGGAGAAGCCGGGTCTGCTCGAGAGCCTGGGCGTGGAGTTCACCGAGCGTGGCAATGTGGC
GCGCGATGACGCGTATGCGACCTCTGTCGATGGCGTGTTCGTGGCCGGGGATATGGGACGCGGACAGTCGCTGATCGTGT
GGGCGATTGCCGAGGGCCGCTCTGCCGCTGCCGCGGTGGACGCGTATCTGACCGGTGAGACGGCGCTTCCGGCGCCGATC
AAGCCGACGGCCGCCCCGCAGCGCTAG

Upstream 100 bases:

>100_bases
GTCAAGGTGATGCCTCGCGACTACCGTCGCGTCCTGGAGGCCATCGCCGACGCGGAGCTGACCGGAGGCGATGTGAACGA
AGCGATCATGGCGGCTGCTC

Downstream 100 bases:

>100_bases
CCGTTCTGCGTGTGAGCGCAGATGTTGCCGGGCTTGCTCCGGTTCGGTAGCTCGCAACGATGTCAACCCCGATTACCGCA
GAGGTTTGCGTGAAGCGGGT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGDPSGFLNHTTRELPKRRPVPLRLLDWKEVYEDFESSRLQIQASRCMDCGIPFCHKGCPLGNLIPEWNDMVYRGNWREG
IERLHATNNFPEFTGRLCPAPCEASCVLGINQDPVTIKQVEVELIDNAFENDWVKPIPPEVKTGKKVAVVGSGPAGLAAA
QQLTRAGHDVTVYERADRIGGLLRYGIPEFKMEKRHIDRRLEQMRAEGTVFEAGVNVGVDITADHLRSNFDAVVLAGGAT
AWRDLPVPGRELEGIYQAMEYLPWANKVQLGDDVVDADGQPPITAKGKRVIIIGGGDTGADCLGTAHRQGAASVQQFEIM
PKPPESRSERDPWPTYPTLFRVASAHEEGGERIYAVNTERFLGEDGKLTGLRAHEVVFNAGKFEKVEGSDFELEADIVFL
AMGFVGPEKPGLLESLGVEFTERGNVARDDAYATSVDGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDAYLTGETALPAPI
KPTAAPQR

Sequences:

>Translated_488_residues
MGDPSGFLNHTTRELPKRRPVPLRLLDWKEVYEDFESSRLQIQASRCMDCGIPFCHKGCPLGNLIPEWNDMVYRGNWREG
IERLHATNNFPEFTGRLCPAPCEASCVLGINQDPVTIKQVEVELIDNAFENDWVKPIPPEVKTGKKVAVVGSGPAGLAAA
QQLTRAGHDVTVYERADRIGGLLRYGIPEFKMEKRHIDRRLEQMRAEGTVFEAGVNVGVDITADHLRSNFDAVVLAGGAT
AWRDLPVPGRELEGIYQAMEYLPWANKVQLGDDVVDADGQPPITAKGKRVIIIGGGDTGADCLGTAHRQGAASVQQFEIM
PKPPESRSERDPWPTYPTLFRVASAHEEGGERIYAVNTERFLGEDGKLTGLRAHEVVFNAGKFEKVEGSDFELEADIVFL
AMGFVGPEKPGLLESLGVEFTERGNVARDDAYATSVDGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDAYLTGETALPAPI
KPTAAPQR
>Mature_487_residues
GDPSGFLNHTTRELPKRRPVPLRLLDWKEVYEDFESSRLQIQASRCMDCGIPFCHKGCPLGNLIPEWNDMVYRGNWREGI
ERLHATNNFPEFTGRLCPAPCEASCVLGINQDPVTIKQVEVELIDNAFENDWVKPIPPEVKTGKKVAVVGSGPAGLAAAQ
QLTRAGHDVTVYERADRIGGLLRYGIPEFKMEKRHIDRRLEQMRAEGTVFEAGVNVGVDITADHLRSNFDAVVLAGGATA
WRDLPVPGRELEGIYQAMEYLPWANKVQLGDDVVDADGQPPITAKGKRVIIIGGGDTGADCLGTAHRQGAASVQQFEIMP
KPPESRSERDPWPTYPTLFRVASAHEEGGERIYAVNTERFLGEDGKLTGLRAHEVVFNAGKFEKVEGSDFELEADIVFLA
MGFVGPEKPGLLESLGVEFTERGNVARDDAYATSVDGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDAYLTGETALPAPIK
PTAAPQR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=470, Percent_Identity=23.8297872340426, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI111118983, Length=123, Percent_Identity=36.5853658536585, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI111118981, Length=123, Percent_Identity=36.5853658536585, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=35.4700854700855, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1788811, Length=469, Percent_Identity=35.181236673774, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082180, Length=454, Percent_Identity=32.5991189427313, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788468, Length=434, Percent_Identity=28.110599078341, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789243, Length=427, Percent_Identity=27.8688524590164, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=495, Percent_Identity=47.4747474747475, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71984108, Length=303, Percent_Identity=27.0627062706271, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=46.9387755102041, Blast_Score=429, Evalue=1e-121,
Organism=Drosophila melanogaster, GI28574881, Length=494, Percent_Identity=51.0121457489879, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24665539, Length=494, Percent_Identity=51.0121457489879, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24665547, Length=494, Percent_Identity=51.0121457489879, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665543, Length=494, Percent_Identity=51.0121457489879, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24640763, Length=470, Percent_Identity=25.1063829787234, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18858217, Length=470, Percent_Identity=25.1063829787234, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53260; Mature: 53129

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDPSGFLNHTTRELPKRRPVPLRLLDWKEVYEDFESSRLQIQASRCMDCGIPFCHKGCP
CCCCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCEEEEEHHHHHHCCCCHHHCCCC
LGNLIPEWNDMVYRGNWREGIERLHATNNFPEFTGRLCPAPCEASCVLGINQDPVTIKQV
CCCCCCCCCCEEEECCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCEEEEE
EVELIDNAFENDWVKPIPPEVKTGKKVAVVGSGPAGLAAAQQLTRAGHDVTVYERADRIG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHH
GLLRYGIPEFKMEKRHIDRRLEQMRAEGTVFEAGVNVGVDITADHLRSNFDAVVLAGGAT
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEHHHHHCCCCEEEEECCCC
AWRDLPVPGRELEGIYQAMEYLPWANKVQLGDDVVDADGQPPITAKGKRVIIIGGGDTGA
CCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCEECCCCCCCCCCCCCEEEEEECCCCCH
DCLGTAHRQGAASVQQFEIMPKPPESRSERDPWPTYPTLFRVASAHEEGGERIYAVNTER
HHHHHHHHCCCCCHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHH
FLGEDGKLTGLRAHEVVFNAGKFEKVEGSDFELEADIVFLAMGFVGPEKPGLLESLGVEF
HHCCCCCEECEEHHHEEECCCCCEECCCCCCEEECCEEEEEECCCCCCCCCHHHHHCCCC
TERGNVARDDAYATSVDGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDAYLTGETALPAPI
HHCCCCCCCCCCEECCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHEEEECCCCCCCCC
KPTAAPQR
CCCCCCCC
>Mature Secondary Structure 
GDPSGFLNHTTRELPKRRPVPLRLLDWKEVYEDFESSRLQIQASRCMDCGIPFCHKGCP
CCCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCEEEEEHHHHHHCCCCHHHCCCC
LGNLIPEWNDMVYRGNWREGIERLHATNNFPEFTGRLCPAPCEASCVLGINQDPVTIKQV
CCCCCCCCCCEEEECCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCEEEEE
EVELIDNAFENDWVKPIPPEVKTGKKVAVVGSGPAGLAAAQQLTRAGHDVTVYERADRIG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHH
GLLRYGIPEFKMEKRHIDRRLEQMRAEGTVFEAGVNVGVDITADHLRSNFDAVVLAGGAT
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEHHHHHCCCCEEEEECCCC
AWRDLPVPGRELEGIYQAMEYLPWANKVQLGDDVVDADGQPPITAKGKRVIIIGGGDTGA
CCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCEECCCCCCCCCCCCCEEEEEECCCCCH
DCLGTAHRQGAASVQQFEIMPKPPESRSERDPWPTYPTLFRVASAHEEGGERIYAVNTER
HHHHHHHHCCCCCHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHH
FLGEDGKLTGLRAHEVVFNAGKFEKVEGSDFELEADIVFLAMGFVGPEKPGLLESLGVEF
HHCCCCCEECEEHHHEEECCCCCEECCCCCCEEECCEEEEEECCCCCCCCCHHHHHCCCC
TERGNVARDDAYATSVDGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDAYLTGETALPAPI
HHCCCCCCCCCCEECCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHEEEECCCCCCCCC
KPTAAPQR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]