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The map label for this gene is kshA [H]

Identifier: 169627185

GI number: 169627185

Start: 76077

End: 77234

Strand: Reverse

Name: kshA [H]

Synonym: MAB_0080c

Alternate gene names: 169627185

Gene position: 77234-76077 (Counterclockwise)

Preceding gene: 169627187

Following gene: 169627184

Centisome position: 1.52

GC content: 60.97

Gene sequence:

>1158_bases
ATGACGACAGCCCAGTCAGCTACCGAATTCGACCCCGCGACCGTTCGGAGCATCGAGGCGGACGCGGCGCCCGCACGGTT
CGCCCGTGGCTGGTATTGCCTCGGCCTCATCAAGGACTTCGGGGATGGAAAACCACATGCCGTCAACGCATTCGGGCAGA
AATTGGTGGTATTTCGCCCGGCAGGCGGCAAGGTCAGTGTTCTGGATGCCTACTGTCGGCACATGGGCGGCGACCTGAGT
CAGGGCGAAGTAAAGGGCGACGAGATAGCCTGTCCCTTCCACGATTGGCGGTGGGGTGGTGACGGACGCTGCAAGAAGGT
CCCCTACAGTCGACGGACCCCTAAACTGGCCCGGACCGCCACCTGGATCACCCTGGAGCAGGACGGCATGCTGTTCATTT
GGCATGACCCGCAGGGCAATCCGCCGCCAGAGGATGTCGCCATTCCCCGTATCGAGGGGGCCACCAGCCCGGAATGGACC
GATTGGCATTGGTACACAACAGTCGTCAATACCAACTGCCGCGAGATCATCGACAACGTGGTGGACATGGCGCACTTCTT
CTACGTGCACGGCGCGGTACCCACCTATTTCAAGAACATCTTCGAAGGACACATCGCCACGCAATACATGAACACCGGTG
AACCGCGTGCCGACCTCCCGATCCCCGAGGGCGTTCAGATGACCGGGCAGACGTCGGTCGCGTCGTACTATGGCCCCTCG
TTCATGATCGACGATCTGACCTACCACTTCGTGGGATACGACTCGAATTCGATCCTGATCAACTGCCACTACCCGATCGA
TGCGAATTCCTTTGTACTGCAATACGGAATCATTGTGAAGAAATCCGCCGGGCTGTCCGACGACGACGCGCTGGCCGCCG
CCATCACGCTGGGGGACTGGATCAAGGCCGGCTTCGAGCAGGACGTACAGATCTGGAAGAACAAGGCGCGCATCGATAAT
CCGCTGCTGTGTGAGGAAGACGGCCCGGTCTATCAGCTACGGCGCTGGTATGAGCAGTTCTATGTGGACGTCGCCGACGT
CACCGCCGAGATGACCGACCGGTTCGAGTACGAGGTGGACACCACTCGCCCTGGCGAGGCGTGGAAGGCCCAGGTCGAGG
AGAACCTCGCGGCGATGGCCGGGGGCGCGCCTTCATGA

Upstream 100 bases:

>100_bases
TTTCCCTCCCAGCGGGAGGTGATCGCAAAAGGGCACAACGATGTCCGGTGAGCGGACCGACCCCCTGGGTCGGCGGTAAA
CCGGCTGTTAGAACAGGTCC

Downstream 100 bases:

>100_bases
TCCGCACGGACAACCGCCTGGCCGATTCGCCGATGGTGCCCGTGGGCTGCGAACGCTGCGGCGCCCAGGTGCTGGCACGC
AAGAGCAGCTGGCAGCAGAC

Product: putative oxygenase

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTTAQSATEFDPATVRSIEADAAPARFARGWYCLGLIKDFGDGKPHAVNAFGQKLVVFRPAGGKVSVLDAYCRHMGGDLS
QGEVKGDEIACPFHDWRWGGDGRCKKVPYSRRTPKLARTATWITLEQDGMLFIWHDPQGNPPPEDVAIPRIEGATSPEWT
DWHWYTTVVNTNCREIIDNVVDMAHFFYVHGAVPTYFKNIFEGHIATQYMNTGEPRADLPIPEGVQMTGQTSVASYYGPS
FMIDDLTYHFVGYDSNSILINCHYPIDANSFVLQYGIIVKKSAGLSDDDALAAAITLGDWIKAGFEQDVQIWKNKARIDN
PLLCEEDGPVYQLRRWYEQFYVDVADVTAEMTDRFEYEVDTTRPGEAWKAQVEENLAAMAGGAPS

Sequences:

>Translated_385_residues
MTTAQSATEFDPATVRSIEADAAPARFARGWYCLGLIKDFGDGKPHAVNAFGQKLVVFRPAGGKVSVLDAYCRHMGGDLS
QGEVKGDEIACPFHDWRWGGDGRCKKVPYSRRTPKLARTATWITLEQDGMLFIWHDPQGNPPPEDVAIPRIEGATSPEWT
DWHWYTTVVNTNCREIIDNVVDMAHFFYVHGAVPTYFKNIFEGHIATQYMNTGEPRADLPIPEGVQMTGQTSVASYYGPS
FMIDDLTYHFVGYDSNSILINCHYPIDANSFVLQYGIIVKKSAGLSDDDALAAAITLGDWIKAGFEQDVQIWKNKARIDN
PLLCEEDGPVYQLRRWYEQFYVDVADVTAEMTDRFEYEVDTTRPGEAWKAQVEENLAAMAGGAPS
>Mature_384_residues
TTAQSATEFDPATVRSIEADAAPARFARGWYCLGLIKDFGDGKPHAVNAFGQKLVVFRPAGGKVSVLDAYCRHMGGDLSQ
GEVKGDEIACPFHDWRWGGDGRCKKVPYSRRTPKLARTATWITLEQDGMLFIWHDPQGNPPPEDVAIPRIEGATSPEWTD
WHWYTTVVNTNCREIIDNVVDMAHFFYVHGAVPTYFKNIFEGHIATQYMNTGEPRADLPIPEGVQMTGQTSVASYYGPSF
MIDDLTYHFVGYDSNSILINCHYPIDANSFVLQYGIIVKKSAGLSDDDALAAAITLGDWIKAGFEQDVQIWKNKARIDNP
LLCEEDGPVYQLRRWYEQFYVDVADVTAEMTDRFEYEVDTTRPGEAWKAQVEENLAAMAGGAPS

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=351, Percent_Identity=26.2108262108262, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI161085896, Length=356, Percent_Identity=26.9662921348315, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: NA

Molecular weight: Translated: 43032; Mature: 42900

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAQSATEFDPATVRSIEADAAPARFARGWYCLGLIKDFGDGKPHAVNAFGQKLVVFRP
CCCCCCCCCCCCHHHHEECCCCCCHHHHCCEEEEEEHHHCCCCCCCEECCCCCEEEEEEC
AGGKVSVLDAYCRHMGGDLSQGEVKGDEIACPFHDWRWGGDGRCKKVPYSRRTPKLARTA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHEE
TWITLEQDGMLFIWHDPQGNPPPEDVAIPRIEGATSPEWTDWHWYTTVVNTNCREIIDNV
EEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
VDMAHFFYVHGAVPTYFKNIFEGHIATQYMNTGEPRADLPIPEGVQMTGQTSVASYYGPS
HHHHHHHHHHCCHHHHHHHHHHHHHHEEHHCCCCCCCCCCCCCCCEECCCCCHHHHCCCC
FMIDDLTYHFVGYDSNSILINCHYPIDANSFVLQYGIIVKKSAGLSDDDALAAAITLGDW
EEEECEEEEEEEECCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEHHHHH
IKAGFEQDVQIWKNKARIDNPLLCEEDGPVYQLRRWYEQFYVDVADVTAEMTDRFEYEVD
HHHCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
TTRPGEAWKAQVEENLAAMAGGAPS
CCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTAQSATEFDPATVRSIEADAAPARFARGWYCLGLIKDFGDGKPHAVNAFGQKLVVFRP
CCCCCCCCCCCHHHHEECCCCCCHHHHCCEEEEEEHHHCCCCCCCEECCCCCEEEEEEC
AGGKVSVLDAYCRHMGGDLSQGEVKGDEIACPFHDWRWGGDGRCKKVPYSRRTPKLARTA
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHEE
TWITLEQDGMLFIWHDPQGNPPPEDVAIPRIEGATSPEWTDWHWYTTVVNTNCREIIDNV
EEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
VDMAHFFYVHGAVPTYFKNIFEGHIATQYMNTGEPRADLPIPEGVQMTGQTSVASYYGPS
HHHHHHHHHHCCHHHHHHHHHHHHHHEEHHCCCCCCCCCCCCCCCEECCCCCHHHHCCCC
FMIDDLTYHFVGYDSNSILINCHYPIDANSFVLQYGIIVKKSAGLSDDDALAAAITLGDW
EEEECEEEEEEEECCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEHHHHH
IKAGFEQDVQIWKNKARIDNPLLCEEDGPVYQLRRWYEQFYVDVADVTAEMTDRFEYEVD
HHHCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
TTRPGEAWKAQVEENLAAMAGGAPS
CCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]