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The map label for this gene is dnaN [H]

Identifier: 169627110

GI number: 169627110

Start: 2169

End: 3368

Strand: Direct

Name: dnaN [H]

Synonym: MAB_0002

Alternate gene names: 169627110

Gene position: 2169-3368 (Clockwise)

Preceding gene: 169627109

Following gene: 169627111

Centisome position: 0.04

GC content: 64.0

Gene sequence:

>1200_bases
ATGGATCTCGCGAGCCCCACTGCCGCAGACACCAGCCTGAAGTTCAGGGTCGCGCGGGACGATTTCGCCGATTCGGTGGC
CTGGGTCGCACGCAGCTTGCCCTCCCGGCCGACGGTTCCGGTGTTGGCCGGCGTGCTGCTGACCGCGCACGACACGGGTT
TGACACTGTCGGGTTTCGACTACGAGGTGTCCGCGCAGGTAAAGGTCCCGGCGGAGGTAGCCGCGGCAGGCAATGCGCTG
GTATCCGGCCGGTTGCTTTCCGATATCACCCGGTCGCTGCCCAACAAGCCCGTCGATGTGGCGCTGGAGGGCACTCGACT
GCTCATCAGTTGTGGGAGCGCCAAGTTCTCGCTGCCGAGCATGCCGGTTGAGGATTACCCGGCTCTGCCCTCGCTGCCGG
AAGAAACGGGTGCGCTGGGTGCCGATGTGTTTTCTGAGGCCATCGGGCAGGTCGCCGTGGCTGCCGGCAAAGACGACACA
CTGCCGATGTTGACCGGTATTCGTGTCGAAATAAACGGTGACACAGTGGTTTTGGCTGCGACTGACCGCTTCCGCTTGGC
GGTCCGTGAGCTGAATTGGACATCGGGGTCCGGTGAAACGAGCGCTGCGGTGCTGGTACCGGCGAAGACGTTGTCGGAAG
CCGCAAAGTCGGCATCCAGTGGGTCGGACGTGCAACTGGCGCTGGGCGCCGGGTCGGCGATCGGATCTGAGGGCCTGCTC
GGTATCGTCAGCGATGGCAAGCGCAGCACTACCCGCCTGCTTGACGCTGAGTTCCCCAAGTTCCGTCAGTTGTTGCCGAC
CGAGCACACGGCCTTGGCGACGATTGCCATCTCGGAGCTGGTTGAGGCCATCAAGCGTGTGGCGTTGGTCGCCGATCGTG
GCGCACAGATCCGGTTGGAGTTTGAGCCGGGGTTGCTGCGGCTGTCGGCGGGTGCGGACGATGTGGGCCGTGCGGAGGAA
GAACTCGAGGTTGAGTTCGTCGGAGAGCCGCTCACGATCGCCTTCAATCCGACGTATCTGACCGACGGGCTGGGGTCGCT
GCACTCGAACCGGGTGACCTTCGGGTTCACCACACCCAGTCGCCCGGCGGTGTTACGTCCCGCTGATGACGATCAAAGCT
CGCCGGAAGGCTCCGGGCCGTTTACGGCGGCGCAAACTGACTATGTCTATCTATTGATGCCGGTGCGTCTGCCCGGCTAG

Upstream 100 bases:

>100_bases
ATGCGCTGTCAGTCTTGTCACCCGGACGTGCCACGATGGCCATCACGGATTTTTGTGTGAACACCGTCCGACCTGGACAA
CGAGGAAGCGAAGGGTATTC

Downstream 100 bases:

>100_bases
GGCTTGCGCTCCTAACAGCATCACGGACTGGTCATCGGAGGTTATGGGTCATGCAGCTGGGTTTGGTCGGGCTAGGCAAG
ATGGGTTTCAACATGCGCGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MDLASPTAADTSLKFRVARDDFADSVAWVARSLPSRPTVPVLAGVLLTAHDTGLTLSGFDYEVSAQVKVPAEVAAAGNAL
VSGRLLSDITRSLPNKPVDVALEGTRLLISCGSAKFSLPSMPVEDYPALPSLPEETGALGADVFSEAIGQVAVAAGKDDT
LPMLTGIRVEINGDTVVLAATDRFRLAVRELNWTSGSGETSAAVLVPAKTLSEAAKSASSGSDVQLALGAGSAIGSEGLL
GIVSDGKRSTTRLLDAEFPKFRQLLPTEHTALATIAISELVEAIKRVALVADRGAQIRLEFEPGLLRLSAGADDVGRAEE
ELEVEFVGEPLTIAFNPTYLTDGLGSLHSNRVTFGFTTPSRPAVLRPADDDQSSPEGSGPFTAAQTDYVYLLMPVRLPG

Sequences:

>Translated_399_residues
MDLASPTAADTSLKFRVARDDFADSVAWVARSLPSRPTVPVLAGVLLTAHDTGLTLSGFDYEVSAQVKVPAEVAAAGNAL
VSGRLLSDITRSLPNKPVDVALEGTRLLISCGSAKFSLPSMPVEDYPALPSLPEETGALGADVFSEAIGQVAVAAGKDDT
LPMLTGIRVEINGDTVVLAATDRFRLAVRELNWTSGSGETSAAVLVPAKTLSEAAKSASSGSDVQLALGAGSAIGSEGLL
GIVSDGKRSTTRLLDAEFPKFRQLLPTEHTALATIAISELVEAIKRVALVADRGAQIRLEFEPGLLRLSAGADDVGRAEE
ELEVEFVGEPLTIAFNPTYLTDGLGSLHSNRVTFGFTTPSRPAVLRPADDDQSSPEGSGPFTAAQTDYVYLLMPVRLPG
>Mature_399_residues
MDLASPTAADTSLKFRVARDDFADSVAWVARSLPSRPTVPVLAGVLLTAHDTGLTLSGFDYEVSAQVKVPAEVAAAGNAL
VSGRLLSDITRSLPNKPVDVALEGTRLLISCGSAKFSLPSMPVEDYPALPSLPEETGALGADVFSEAIGQVAVAAGKDDT
LPMLTGIRVEINGDTVVLAATDRFRLAVRELNWTSGSGETSAAVLVPAKTLSEAAKSASSGSDVQLALGAGSAIGSEGLL
GIVSDGKRSTTRLLDAEFPKFRQLLPTEHTALATIAISELVEAIKRVALVADRGAQIRLEFEPGLLRLSAGADDVGRAEE
ELEVEFVGEPLTIAFNPTYLTDGLGSLHSNRVTFGFTTPSRPAVLRPADDDQSSPEGSGPFTAAQTDYVYLLMPVRLPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=392, Percent_Identity=28.8265306122449, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41638; Mature: 41638

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLASPTAADTSLKFRVARDDFADSVAWVARSLPSRPTVPVLAGVLLTAHDTGLTLSGFD
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCEEECCC
YEVSAQVKVPAEVAAAGNALVSGRLLSDITRSLPNKPVDVALEGTRLLISCGSAKFSLPS
EEEEEEEECCHHHHHCCCCEECCHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCC
MPVEDYPALPSLPEETGALGADVFSEAIGQVAVAAGKDDTLPMLTGIRVEINGDTVVLAA
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHEEEECCCCCCCEEEEEEEEEECCCEEEEEE
TDRFRLAVRELNWTSGSGETSAAVLVPAKTLSEAAKSASSGSDVQLALGAGSAIGSEGLL
CCCEEEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCE
GIVSDGKRSTTRLLDAEFPKFRQLLPTEHTALATIAISELVEAIKRVALVADRGAQIRLE
EEECCCCCCHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEEE
FEPGLLRLSAGADDVGRAEEELEVEFVGEPLTIAFNPTYLTDGLGSLHSNRVTFGFTTPS
ECCCEEEEECCCCCCCCCCHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
RPAVLRPADDDQSSPEGSGPFTAAQTDYVYLLMPVRLPG
CCEEECCCCCCCCCCCCCCCEEECCCCEEEEEEEEECCC
>Mature Secondary Structure
MDLASPTAADTSLKFRVARDDFADSVAWVARSLPSRPTVPVLAGVLLTAHDTGLTLSGFD
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCEEECCC
YEVSAQVKVPAEVAAAGNALVSGRLLSDITRSLPNKPVDVALEGTRLLISCGSAKFSLPS
EEEEEEEECCHHHHHCCCCEECCHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCC
MPVEDYPALPSLPEETGALGADVFSEAIGQVAVAAGKDDTLPMLTGIRVEINGDTVVLAA
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHEEEECCCCCCCEEEEEEEEEECCCEEEEEE
TDRFRLAVRELNWTSGSGETSAAVLVPAKTLSEAAKSASSGSDVQLALGAGSAIGSEGLL
CCCEEEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCE
GIVSDGKRSTTRLLDAEFPKFRQLLPTEHTALATIAISELVEAIKRVALVADRGAQIRLE
EEECCCCCCHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEEE
FEPGLLRLSAGADDVGRAEEELEVEFVGEPLTIAFNPTYLTDGLGSLHSNRVTFGFTTPS
ECCCEEEEECCCCCCCCCCHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
RPAVLRPADDDQSSPEGSGPFTAAQTDYVYLLMPVRLPG
CCEEECCCCCCCCCCCCCCCEEECCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8733228 [H]