Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 169834479

GI number: 169834479

Start: 273523

End: 274773

Strand: Direct

Name: folC [H]

Synonym: SPH_0313

Alternate gene names: 169834479

Gene position: 273523-274773 (Clockwise)

Preceding gene: 169833153

Following gene: 169833963

Centisome position: 12.18

GC content: 43.8

Gene sequence:

>1251_bases
ATGTTTGAAGTAGAAGAATGGCTCCATAGTCGGATTGGTTTAAATTTTCGATCAGGTTTGGGCCGAATGCAGCAAGCGGT
GGATTTGTTAGGAAATCCTGAGCAGTCTTACCCTATTATCCACGTAACAGGGACTAATGGGAAAGGATCTACCATTGCTT
TTATGAGGGAATTATTTATGGGGCATGGCAAAAAAGTTGCGACCTTTACCTCCCCTCATATCGTCTCTATCAATGACCGA
ATCTGCATTAATGGGCAACCTATAGCAGATGCAGACTTTATCCGTTTGGCTGATCAGGTAAAGGAGATGGAGAAAACGCT
TCTGCAAACTCCTGACCAGTTGTCCTTTTTTGAATTGCTGACCTTGGTTGCTTTTCTTTATTTTAGGGAGCAGGAGGTGG
ATTTGGTTTTATTAGAAGTGGGAATTGGTGGCTTACTTGACACGACCAATGTGGTAACTGGAGAGCTTGCTGTCATCACC
TCCATTGGGCTTGACCATCAAGAAACCTTGGGTGATAGTCTAGAAGCAATTGCAGAGCAGAAAGCTGGTATTTTCAAGGC
TGGTAAAAAGGCAGTGATTGCGAAATTGCCTCCAGAAGCTAGACTTGTCTGTCAGAAAAAAGCCGAATCTTTAGCTGTTG
ACCTTTATCAGGCAGGTCAAGATTTTTCAGTGCTGAATGGTGATTTTTCAAGCTCTTTACTAAATCTTTCACAGCTGAAC
ATAGGCTTAGAAGGAGCCTATCAGCAGGAGAATGCGGCCTTGGCGTTGCAAACTTTTCTTCTTTTTATGGGAGAAAGAAA
GGAAGCTATTGATGAACAGGCTGTAAGAAAGGCCTTGGAACAGACCCATTGGGCTGGTCGCTTGGAGCGTATTCGCCCAC
AGATTTACTTGGATGGTGCTCATAACCTCCCTGCCTTGACTCGCTTGGTTGAGTTTATCAAAGAAAAAGAGCAGGAAGGC
TATCGACCTCAAATCCTCTTTGGATCCTTGAAACGTAAGGATTATCAAGGGATGTTGGGTTATCTGACTGAAAATTTACC
TCAAGTGGAACTCAAAATGACCGGCTTTGACTATCAAGGGTCTTTGGACGAGACAGATGTAACAGGTTACCATGTAATTC
CCTCTTACCGAGAATTTATCAGCAGTTTCGAAGAAAGGGCAGACGCTCAAGACTTGCTGTTCGTTACAGGGTCTCTCTAT
TTTATCTCAGAAGTACGGGGCTACCTGCTGGACCGTGAGCAGATAAATTGA

Upstream 100 bases:

>100_bases
CTAACCAGGTAAGAGGTCGGGACGAAAATCCTGGCCTTGTTTTTATTAGTAACCATACTTTAATGCGATAATTGATTGGA
CTTTTGTCAAGGAGATGTGT

Downstream 100 bases:

>100_bases
CCCCCTTTTTTGAACTTGTTATACTAGGGGAATTGCTGGTTAGAGAAAATTTCTCTAAATTGGTAGCAGAAAGGAAATTC
ATCATGAAATTAAAAAGATT

Product: dihydrofolate:folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDR
ICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQAGQDFSVLNGDFSSSLLNLSQLN
IGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVRKALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEG
YRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLY
FISEVRGYLLDREQIN

Sequences:

>Translated_416_residues
MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDR
ICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQAGQDFSVLNGDFSSSLLNLSQLN
IGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVRKALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEG
YRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLY
FISEVRGYLLDREQIN
>Mature_416_residues
MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDR
ICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQAGQDFSVLNGDFSSSLLNLSQLN
IGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVRKALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEG
YRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLY
FISEVRGYLLDREQIN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=372, Percent_Identity=33.0645161290323, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI66932990, Length=342, Percent_Identity=33.9181286549708, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1788654, Length=341, Percent_Identity=31.6715542521994, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=429, Percent_Identity=27.2727272727273, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=429, Percent_Identity=27.2727272727273, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=429, Percent_Identity=27.2727272727273, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=28.3018867924528, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=340, Percent_Identity=28.8235294117647, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322718, Length=287, Percent_Identity=22.9965156794425, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641571, Length=308, Percent_Identity=33.1168831168831, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581568, Length=327, Percent_Identity=25.0764525993884, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46607; Mature: 46607

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFM
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
GHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELL
CCCCEEEEECCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVITSIGLDHQETLGDSLEAIAEQ
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHH
KAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQAGQDFSVLNGDFSSSLLNLSQLN
HCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHC
IGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVRKALEQTHWAGRLERIRPQIYLDGA
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
HNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQG
CCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
SLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLYFISEVRGYLLDREQIN
CCCCCCCCCEEECHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHHCHHCCC
>Mature Secondary Structure
MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFM
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
GHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELL
CCCCEEEEECCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVITSIGLDHQETLGDSLEAIAEQ
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHH
KAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQAGQDFSVLNGDFSSSLLNLSQLN
HCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHC
IGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVRKALEQTHWAGRLERIRPQIYLDGA
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
HNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQG
CCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
SLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLYFISEVRGYLLDREQIN
CCCCCCCCCEEECHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHHCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]