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The map label for this gene is nrdE [H]

Identifier: 169834419

GI number: 169834419

Start: 1205288

End: 1207447

Strand: Direct

Name: nrdE [H]

Synonym: SPH_1300

Alternate gene names: 169834419

Gene position: 1205288-1207447 (Clockwise)

Preceding gene: 169834064

Following gene: 169833110

Centisome position: 53.67

GC content: 40.97

Gene sequence:

>2160_bases
ATGGGATTAAAACATCTTGAAGACGTGACTTACTTCCGTCTCAATAACGAAATCAACCGTCCTGTCAATGGACAAATCAT
GCTTCATAAAGATAAGGAAGCCTTGGATGCTTTCTTTAAAGAAAATGTAGTTCCAAACACTATGGTTTTTGATTCAATCA
AAGATAAAATCAACTACCTCATTGAACACAACTACATAGAAACAGCCTTTATCAAGAAATACCGTCCAGAATTTTTGGAA
GAATTGTCACAATTTATCAAAGACCAAAACTTCCAATTCAAGTCTTTCATGGCTGCTTATAAATTTTACAATCAATATGC
CTTAAAGACTAATGACGGTGAATACTATCTTGAAAGTATGGAAGACCGTGTCTTCTTTAACGCCCTTTATTTCGCTGATG
GGAATGAAGCTGTTGCAATCGATATTGCCAATGAAATCATCCACCAACGCTACCAACCTGCTACTCCTTCCTTCTTGAAT
GCTGGACGTGCTCGTCGTGGGGAGTTGGTATCTTGTTTCTTAATCCAAGTGACGGATGACATGAACTCTATCGGACGTTC
TATCAACTCTGCCCTTCAACTTTCACGTATTGGTGGTGGTGTGGGAATTACCCTCAGCAACCTTCGTGAAGCTGGTGCAC
CTATCAAAGGCTATGAAGGAGCTGCTTCAGGTGTCGTACCAGTTATGAAACTCTTCGAAGATAGCTTCTCTTACTCAAAC
CAATTGGGTCAACGTCAAGGTGCTGGGGTGGTTTACCTCAATGTCTTTCACCCAGATATCATTGCTTTCCTTTCAACTAA
GAAAGAAAACGCTGATGAAAAAGTACGTGTCAAGACTCTATCACTTGGTGTTGTAGTACCTGATAAATTCTACGAATTGG
CGCGTAAAAATGAAGAAATGTACCTCTTCAGCCCTTACTCTGTAGAAAAAGAATACAATGTGCCATTCAACTACATCGAT
ATTACTGAAAAATACGATGAATTGGTCGCAAATCCAAATATTCGCAAGACAAAAATCAAGGCGCGTGATTTAGAAACTGA
AATCTCTAAATTACAGCAAGAGTCTGGCTACCCTTATGTCGTCAACATTGATACGGCTAACCGTGCAAATCCTGTTGATG
GAAAGATTATCATGAGTAACTTGTGTTCTGAGATTCTTCAAGTTCAAGAACCAAGCTTGATCAACGATGCTCAAGAATTC
CTTCAAATGGGAACGGACGTTTCATGTAACCTTGGTTCAACCAACGTAGTCAACATGATGACTTCACCTGACTTTGGTCG
TTCTATCCGTGCTATGGTTCGTGCCCTTACTTTCGTTACAGATAGTTCGCACATCGTAGCTGTTCCTACTATCGACCATG
GAAATAGCCAAGCTCATACCTTTGGTCTTGGTGCCATGGGACTTCACAGCTACCTTGCCCAACAACTGATTGAATATGGT
TCACCTGAGTCTGTTGAATTTACAAGCATCTACTTTATGCTTATGAACTACTGGACTTTGGTTGAGTCAAACAATATTGC
GCGTGAACGTGGTATCACTTTCCACAATTTTGAAAAATCAGATTATGCTAAGGGAAGCTACTTTGACAAGTATGTAACTG
GCGAATTTGTTCCAAAATCAGACCGTGTTAAAGAACTTTTCAAAGATGTCTTTATCCCAAGTGCTGCTGACTGGGCTGAA
CTTCGAGACAAGGTTCAAGCAGATGGACTTTACCACCAAAACCGTCTAGCTGTTGCTCCAAATGGTTCGATCAGTTACAT
CAATGACGTTTCTGCCTCTATCCACCCGATTACGCAACGTATCGAAGAACGTCAAGAAAAGAAAATCGGTAAAATCTACT
ATCCTGCTGCTGGCTTGTCAACAGAAACCATTCCTTACTACACTTCTGCTTACGACATGGATATGCGTAAGGTGATTGAT
GTTTACGCTGCTGCGACTGAGCACGTGGACCAAGGACTTTCACTCACACTCTTTATGCGTAGTGACATTCCAAAAGGACT
TTACGAATGGAAGAGGGAAAATAAACAAACGACACGTGACTTGTCTATCCTTCGTAACTATGCCTTTAACAAGGGTATCA
AGTCTATCTACTACGTCCGTACCTTTACAGATGATGGTGGAGAAGTCGGTGCCAACCAATGTGAAAGCTGTGTGATTTAG

Upstream 100 bases:

>100_bases
AACTGAAACAATTAGCATAATCTTAGTACATCATCCAGAAGAAACTGCTTCCAGGGCTAACTTAGAAGCCTTTCTTTTGT
AATTAGATAAGGGAAATTTT

Downstream 100 bases:

>100_bases
TTTCTAGCATAGAAACACTATTTTTCCCTAAGCAGCACTGATAAAATCAAATCTACTAGGCCATAACATAATTTATGTAA
CATTTTAAAAGTCGGGCTTC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLE
ELSQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLESMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYNVPFNYID
ITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEF
LQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYAKGSYFDKYVTGEFVPKSDRVKELFKDVFIPSAADWAE
LRDKVQADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI

Sequences:

>Translated_719_residues
MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLE
ELSQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLESMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYNVPFNYID
ITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEF
LQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYAKGSYFDKYVTGEFVPKSDRVKELFKDVFIPSAADWAE
LRDKVQADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI
>Mature_718_residues
GLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEE
LSQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLESMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNA
GRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSNQ
LGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYNVPFNYIDI
TEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFL
QMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYGS
PESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYAKGSYFDKYVTGEFVPKSDRVKELFKDVFIPSAADWAEL
RDKVQADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDV
YAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=708, Percent_Identity=24.5762711864407, Blast_Score=153, Evalue=7e-37,
Organism=Escherichia coli, GI1789030, Length=718, Percent_Identity=48.8857938718663, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=693, Percent_Identity=24.3867243867244, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=630, Percent_Identity=25.0793650793651, Blast_Score=161, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322123, Length=660, Percent_Identity=24.2424242424242, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6320914, Length=672, Percent_Identity=23.0654761904762, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI17352481, Length=519, Percent_Identity=25.8188824662813, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81625; Mature: 81494

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYL
CCCCHHCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IEHNYIETAFIKKYRPEFLEELSQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLESM
HHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHH
EDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNAGRARRGELVSCFLIQVTDD
HCCEEEEEEEEECCCCEEEEEEHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECCH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYSVEKEYNVPFNYIDITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYV
EEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEE
VNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFLQMGTDVSCNLGSTNVVNMM
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEC
TSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
CCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCC
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYAKGSYFDKYVTGEFVPKS
CCCCCHHHHHHHHHHHHHHHEECCCCHHHCCCEEECCCCCCCCCCCCCHHHCCCCCCCCH
DRVKELFKDVFIPSAADWAELRDKVQADGLYHQNRLAVAPNGSISYINDVSASIHPITQR
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCHHHHHHHH
IEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHCCC
>Mature Secondary Structure 
GLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYL
CCCHHCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IEHNYIETAFIKKYRPEFLEELSQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLESM
HHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHH
EDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNAGRARRGELVSCFLIQVTDD
HCCEEEEEEEEECCCCEEEEEEHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECCH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYSVEKEYNVPFNYIDITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYV
EEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEE
VNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFLQMGTDVSCNLGSTNVVNMM
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEC
TSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYG
CCCCCHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCC
SPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYAKGSYFDKYVTGEFVPKS
CCCCCHHHHHHHHHHHHHHHEECCCCHHHCCCEEECCCCCCCCCCCCCHHHCCCCCCCCH
DRVKELFKDVFIPSAADWAELRDKVQADGLYHQNRLAVAPNGSISYINDVSASIHPITQR
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCHHHHHHHH
IEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]