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The map label for this gene is purN [H]

Identifier: 169834363

GI number: 169834363

Start: 130971

End: 131516

Strand: Direct

Name: purN [H]

Synonym: SPH_0155

Alternate gene names: 169834363

Gene position: 130971-131516 (Clockwise)

Preceding gene: 169833754

Following gene: 169834382

Centisome position: 5.83

GC content: 47.25

Gene sequence:

>546_bases
ATGAAAAAAATAGCGGTTTTTGCCTCTGGTAATGGCTCAAATTTTCAGGTGATTGCCGAAGAATTTCCAGTGGAGTTTGT
CTTTTCAGACCATCGTGATGCCTATGTGCTTGAGCGTGCAAAGCAGCTCGGCGTTCTGTCCTATGCTTTTGAACTCAAGG
AGTTTGAGAGCAAGGCAGACTACGAAGCAGCCCTTGTCGAACTCTTGGAAGAACACCAGATTGACTTGGTTTGCCTAGCA
GGCTACATGAAAATCGTTGGACCAACCTTATTGTCGGCTTATGAAGGTCGGATTGTCAACATTCATCCAGCCTACTTGCC
AGAATTTCCAGGAGCTCATGGGATTGAGGATGCTTGGAATGCTGGCGTGGGTCAGTCTGGTGTGACCATTCACTGGGTGG
ATTCGGGTGTGGATACAGGCCAGGTCATCAAACAGGTTCGTGTGCCACGACTAGCTGATGATACCATTGACAGATTTGAA
GCTCGCATCCATGAAGCAGAGTACAGGTTGTATCCGGAAGTAGTGAAGGCTCTATTTACAGATTGA

Upstream 100 bases:

>100_bases
TCAGCCCTGAAAATGTAGAGCGTGTAAAAGAATTGTTGGATGAAGCAGTCTATGAAATTGGTCGCATCGTCAAGAAAGAA
AACGAAAGTGTCATTATCAA

Downstream 100 bases:

>100_bases
CTTTTTGATGATTCATATGATATCTTTGATTTTAAATTGGAGTCAGTGTTTGTTGAAGACGGCTTCAAACGGAGGTATTT
GTAATGTTAGAATCTAAAAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLGVLSYAFELKEFESKADYEAALVELLEEHQIDLVCLA
GYMKIVGPTLLSAYEGRIVNIHPAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
ARIHEAEYRLYPEVVKALFTD

Sequences:

>Translated_181_residues
MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLGVLSYAFELKEFESKADYEAALVELLEEHQIDLVCLA
GYMKIVGPTLLSAYEGRIVNIHPAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
ARIHEAEYRLYPEVVKALFTD
>Mature_181_residues
MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLGVLSYAFELKEFESKADYEAALVELLEEHQIDLVCLA
GYMKIVGPTLLSAYEGRIVNIHPAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
ARIHEAEYRLYPEVVKALFTD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=181, Percent_Identity=32.0441988950276, Blast_Score=119, Evalue=1e-27,
Organism=Homo sapiens, GI209869995, Length=181, Percent_Identity=32.0441988950276, Blast_Score=119, Evalue=1e-27,
Organism=Homo sapiens, GI209869993, Length=181, Percent_Identity=32.0441988950276, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=37.5690607734807, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787483, Length=98, Percent_Identity=32.6530612244898, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=34.4262295081967, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=28.5, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=38.8297872340425, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20246; Mature: 20246

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLGVLSYAFELKEFESKAD
CCEEEEEECCCCCCEEEEHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIHPAYLPEFPGAHGIEDAWN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHC
AGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFEARIHEAEYRLYPEVVKALFT
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
D
C
>Mature Secondary Structure
MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLGVLSYAFELKEFESKAD
CCEEEEEECCCCCCEEEEHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIHPAYLPEFPGAHGIEDAWN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHC
AGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFEARIHEAEYRLYPEVVKALFT
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]