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The map label for this gene is vanSB [H]

Identifier: 169834135

GI number: 169834135

Start: 644670

End: 645998

Strand: Direct

Name: vanSB [H]

Synonym: SPH_0698

Alternate gene names: 169834135

Gene position: 644670-645998 (Clockwise)

Preceding gene: 169833674

Following gene: 169834354

Centisome position: 28.71

GC content: 43.34

Gene sequence:

>1329_bases
ATGAAACGAACAGGTTTATTTACAAAGATATTTATCTATACCTTCTCGATATTTAGTGTTCTGGTTATCTGCCTTCATTT
AGCTATTTATTTTCTTTTTCCTTCGACTTATCTGAGTCATCGTCAGGAAACCATTGGTCAAAAGGCAACAGCCATTGCCC
AGTCCCTAGAAGGGAAAGATAGGCAGAGTATCGAGCAAGTGTTAGACTTGTATTCCCAGACTAGTGATATCAAGGGGACC
GTCAAAGGTGAGATGACCGAGGACAAGTTAGAAGTCAAGGACAGTCTTCCTCTGGACACAGACCGCCAGACAACCTCTCT
CTTTATTGAGGAGCGCGAGGTGAAAACGCAAGACGGTGATACTATGATTCTCCAGTTTCTAGCTTCCATGGATTTACAAA
AGGAAGCGGAGCAAATCAGTCTCCAGTTTCTTCCCTATACCTTGCTGGCCTCCTTTCTGATTTCCCTCTTGGTGGCCTAC
ATCTACGCTCGGACTATTGTTGCACCGATTTTGGAAATCAAGCGGGTGACCCGTCGGATGATGGACCTGGATTCCCAAGT
GCGATTGCGCGTGGATTCTAAGGATGAGATAGGTAATCTCAAGGAACAAATCAATAGCCTCTACCAGCATCTCTTGACTG
TTATTGCGGACTTGCATGAAAAGAATGAAGCCATTCTCCAGCTGGAGAAGATGAAGGTCGAATTCCTACGAGGAGCTTCT
CATGAATTGAAAACACCGCTGGCTAGTTTGAAAATCCTAATCGAAAATATGAGAGAGAATATCGGTCGTTATAAGGATAG
AGACCAGTATCTGGGAGTTGCCTTGGGGATTGTGGATGAACTCAATCACCATGTTCTGCAGATACTTTCCCTCTCTTCTG
TGCAGGAATTGCGAGATGATAGGGAAACAATTGACCTCCTCCAGATGACGAAAAATCTGGTCAAAGATTATGCCTTGCTA
GCCAAGGAAAGAGAGCTCCAGATAGACAATAGTTTGACCCATCAGCAGGCTTATCTAAACCCATCAGTTATGAAGTTGAT
TCTTTCTAATCTCATCAGCAATGCCATTAAGCACTCTGTTCCAGGTGGCTTAGTTCGAATTGGAGAAAGAGAAGGAGAAC
TTTTTATCGAAAATAGCTGTAGCTCAGAGGAACAAGAAAAACTAGCCCAGTCTTTTTCTGACAATGCTAGTCGCAAGGTC
AAGGGGTCTGGTATGGGGCTCTTTGTGGTTAAGAGTCTATTAGAACATGAAAAATTAGCTTATCGTTTCGAGATGGAGGA
GAATCGTTTAACCTTCTTTATAGCTTTTCCAAAAGTCGCCCAAGACTAG

Upstream 100 bases:

>100_bases
TTGACCGTGTTATTGATGTTTATATCAAGGAATTGCGGAAAAAGCTAGACTTGGATTGTATCCTCACTGTGCGCAATGTT
GGTTATAAATTGGAGCGAAA

Downstream 100 bases:

>100_bases
GGAGAGAAAGGGTTTACATAGATGGAGTTAGAAGAAAATCAATCGAAACTGCGGGAAAAACTAGATTTTTTTGGCAAAAA
GTGATAAAATGAACAATGTA

Product: VncS, histidine kinase

Products: NA

Alternate protein names: Vancomycin B-type resistance protein vanSB; Vancomycin histidine protein kinase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQETIGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGT
VKGEMTEDKLEVKDSLPLDTDRQTTSLFIEEREVKTQDGDTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADLHEKNEAILQLEKMKVEFLRGAS
HELKTPLASLKILIENMRENIGRYKDRDQYLGVALGIVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALL
AKERELQIDNSLTHQQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSFSDNASRKV
KGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD

Sequences:

>Translated_442_residues
MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQETIGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGT
VKGEMTEDKLEVKDSLPLDTDRQTTSLFIEEREVKTQDGDTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADLHEKNEAILQLEKMKVEFLRGAS
HELKTPLASLKILIENMRENIGRYKDRDQYLGVALGIVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALL
AKERELQIDNSLTHQQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSFSDNASRKV
KGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD
>Mature_442_residues
MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQETIGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGT
VKGEMTEDKLEVKDSLPLDTDRQTTSLFIEEREVKTQDGDTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADLHEKNEAILQLEKMKVEFLRGAS
HELKTPLASLKILIENMRENIGRYKDRDQYLGVALGIVDELNHHVLQILSLSSVQELRDDRETIDLLQMTKNLVKDYALL
AKERELQIDNSLTHQQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSFSDNASRKV
KGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=325, Percent_Identity=24.9230769230769, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=303, Percent_Identity=24.7524752475248, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50542; Mature: 50542

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQETIGQKATAIAQSLEGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDRQTTSLFIEEREVKTQDGD
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCH
TMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAYIYARTIVAPILEIKRVTRRM
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADLHEKNEAILQLEKMKVEFLRGAS
HCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HELKTPLASLKILIENMRENIGRYKDRDQYLGVALGIVDELNHHVLQILSLSSVQELRDD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RETIDLLQMTKNLVKDYALLAKERELQIDNSLTHQQAYLNPSVMKLILSNLISNAIKHSV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
PGGLVRIGEREGELFIENSCSSEEQEKLAQSFSDNASRKVKGSGMGLFVVKSLLEHEKLA
CCCEEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHH
YRFEMEENRLTFFIAFPKVAQD
HHEEECCCCEEEEEECCHHCCC
>Mature Secondary Structure
MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQETIGQKATAIAQSLEGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDRQTTSLFIEEREVKTQDGD
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCH
TMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAYIYARTIVAPILEIKRVTRRM
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADLHEKNEAILQLEKMKVEFLRGAS
HCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HELKTPLASLKILIENMRENIGRYKDRDQYLGVALGIVDELNHHVLQILSLSSVQELRDD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RETIDLLQMTKNLVKDYALLAKERELQIDNSLTHQQAYLNPSVMKLILSNLISNAIKHSV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
PGGLVRIGEREGELFIENSCSSEEQEKLAQSFSDNASRKVKGSGMGLFVVKSLLEHEKLA
CCCEEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHH
YRFEMEENRLTFFIAFPKVAQD
HHEEECCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]