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The map label for this gene is gmuD [H]

Identifier: 169834029

GI number: 169834029

Start: 1999220

End: 2000629

Strand: Direct

Name: gmuD [H]

Synonym: SPH_2175

Alternate gene names: 169834029

Gene position: 1999220-2000629 (Clockwise)

Preceding gene: 169834394

Following gene: 169832416

Centisome position: 89.03

GC content: 38.58

Gene sequence:

>1410_bases
ATGAAAAAAAAGTTAGTATTTCCTAATCTGTTTTGGTGGGGGGCTGCTTCTAGCGGACCTCAGACAGAAGGTCAATATGG
AAAAGTACATGAAAATGTGATGGACTACTGGTTCAAAACGCATCCAGAAGATTTTTTCGATAATGTCGGACCTCTTGTAG
CCAGTAACTTTTTTCATACTTACACCGAAGATTTCCACTTGATGAAGGAAATTGGAGTTAATTCTTTCCGCACTTCCATC
CAATGGAGTCGACTCATCAAGAATTTAGAGACAGGTGAGCCTGATCCAAAAGGTATTGCTTTCTACAATGCCATCATTGA
AGAAGCTAAAAAGAACCAGATGGATCTTGTGATGAATTTACATCATTTTGATTTACCAGTGGAACTTCTTCAAAAATACG
GTGGTTGGGAAAGCAAACATGTAGTGGAGTTATTCGTGAAGTTTGCCAAGACTGCTTTCACATGCTTTGGAGATAAGGTT
CATTACTGGACAACTTTCAATGAGCCAATGGTCATTCCAGAAGCAGGATACTTATATGCTTTCCATTATCCAAATCTAAA
AGGAAAGGGAAAAGAGGCCGTACAAATCATCTATAATCTAAACCTTGCTAGTGCAAAAGTGATTCAACTATATCGCTCAT
TAGGACTTGATGGAAAGATTGGGATTATTTTAAACTTGACACCTGCTTATCCAAGAAGTAATTCTCCAGAAGACTTAGAA
GCAAGTCGATTTACAGATGACTTCTTTAACAAAGTCTTCTTGAATCCAGCTGTTAAAGGAACTTTCCCAGAAAGATTGGT
AAAACAGCTAGAGAGAGATGGCGTGTTATGGAGTCATACCGAAAAAGAGCTTCAACTGATGAAATCAAATACGGTTGATT
TTCTTGGAGTAAACTACTACCATCCAAAACGTGTTCAAGCACAAGCAAATCCTGAGGAATATCAGACGCCCTGGATGCCA
GACCAATACTTCAAAGAGTATGAATGGCCGGAGCGTCGCATGAATCCATATCGTGGTTGGGAAATTTTTCCGAAAGCCAT
TTATGATATTGCTATGATTGTGAAGGAAGAATATGATAATATTCCATGGTTTATCAGTGAAAACGGAATGGGTGTTGAAA
ACGAAGCACGGTTTATCGGTGAAAATGGAGTTATCGATGACGTGTATCGTATTGAATTTTATGAAGAACATTTAAGATGG
CTACATAAAGCCATTGAAGAGGGAAGTCACTGTTTTGGATACCACGCTTGGACCGCATTTGATTGCTGGTCTTGGAATAA
TGCATATAAGAATCGTTACGGATTTATCTCCGTTGATTTAGAAACGCAAAAGAGAACCATCAAGAGCTCAGGAAGATGGT
ATCGCAAAGTAAGTGACAATAACGGTTTTGAAGTAGAAATTGAGGAGTAA

Upstream 100 bases:

>100_bases
GGCAAACACAAGATATGTATCAACAATTTTTAAAAATGTCTAGACAAATTTTAAAATTATGCTATACTTTATGTATAGAA
TAATAGAAAGGAGGTTGAGC

Downstream 100 bases:

>100_bases
AGAGAGGAGATATTCAGATGAAAAACTCAAAATTTATAGATCAATTTGCCACCTTTGCTGGTAAACTAGGGAACCAAATT
CATTTAAAAACCCTAAGAGA

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSI
QWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDGKIGIILNLTPAYPRSNSPEDLE
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMP
DQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIEFYEEHLRW
LHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE

Sequences:

>Translated_469_residues
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSI
QWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDGKIGIILNLTPAYPRSNSPEDLE
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMP
DQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIEFYEEHLRW
LHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE
>Mature_469_residues
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSI
QWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDGKIGIILNLTPAYPRSNSPEDLE
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMP
DQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIEFYEEHLRW
LHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=490, Percent_Identity=29.5918367346939, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI110681710, Length=483, Percent_Identity=27.7432712215321, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=29.6523517382413, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI24497614, Length=483, Percent_Identity=26.9151138716356, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI28376633, Length=475, Percent_Identity=25.6842105263158, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1789070, Length=494, Percent_Identity=30.3643724696356, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI2367174, Length=489, Percent_Identity=29.8568507157464, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI2367270, Length=490, Percent_Identity=28.3673469387755, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17539390, Length=469, Percent_Identity=30.0639658848614, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17552856, Length=478, Percent_Identity=28.4518828451883, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21356577, Length=486, Percent_Identity=27.1604938271605, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55140; Mature: 55140

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHT
CCCCEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
YTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEH
HHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKVHYWTTFNEPMVIPEAGYLYA
HHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCEEEE
FHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDGKIGIILNLTPAYPRSNSPEDLE
EECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCH
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCC
HPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYDN
CCCCEECCCCHHHCCCCCCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
IPWFISENGMGVENEARFIGENGVIDDVYRIEFYEEHLRWLHKAIEEGSHCFGYHAWTAF
CCEEECCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
DCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE
EECCCCCHHCCCCEEEEEEEHHHHHHHHCCCCEEEEECCCCCEEEEECC
>Mature Secondary Structure
MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPEDFFDNVGPLVASNFFHT
CCCCEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
YTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEH
HHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKVHYWTTFNEPMVIPEAGYLYA
HHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCEEEE
FHYPNLKGKGKEAVQIIYNLNLASAKVIQLYRSLGLDGKIGIILNLTPAYPRSNSPEDLE
EECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCH
ASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCC
HPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRMNPYRGWEIFPKAIYDIAMIVKEEYDN
CCCCEECCCCHHHCCCCCCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
IPWFISENGMGVENEARFIGENGVIDDVYRIEFYEEHLRWLHKAIEEGSHCFGYHAWTAF
CCEEECCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEE
DCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE
EECCCCCHHCCCCEEEEEEEHHHHHHHHCCCCEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]