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The map label for this gene is dnaG [H]

Identifier: 169834027

GI number: 169834027

Start: 1067038

End: 1068798

Strand: Direct

Name: dnaG [H]

Synonym: SPH_1160

Alternate gene names: 169834027

Gene position: 1067038-1068798 (Clockwise)

Preceding gene: 169832401

Following gene: 169833634

Centisome position: 47.52

GC content: 41.62

Gene sequence:

>1761_bases
ATGGTTGACAAACAAGTCATTGAAGAAATCAAAAACAATGCCAACATTGTGGAAGTCATAGGAGATGTGATTTCTTTACA
AAAGGCAGGACGGAACTATCTAGGGCTCTGTCCTTTTCATGGTGAAAAAACACCTTCTTTCAACGTTGTAGAGGATAAGC
AGTTTTATCACTGTTTCGGTTGTGGTCGCTCAGGTGATGTCTTTAAGTTCATAGAGGAGTACCAAGGGGTTCCCTTTATA
GAGGCAGTCCAAATCTTAGGTCAGCGTGTTGGAATTGAGGTTGAAAAACCGCTTTATAGTGAGCAAAAGTCAGCCTCACC
TCACCAAGATCTTTATGATATGCACGAAGATGCAGCCAAATTTTACCATGCTATTCTCATGACAACGACTATGGGCGAAG
AGGCCAGAAATTACCTTTATCAGCGGGGTTTGACAGATGAAGTGCTTAAACATTTTTGGATTGGTTTAGCACCTCCAGAA
CGAAACTATCTCTATCAACGTTTGTCTGATCAGTATCGTGAAGAGGATTTACTGGATTCAGGCCTGTTTTATCTCTCGGA
TGCCAATCAATTTGTAGACACCTTTCACAATCGCATTATGTTTCCCCTGACAAATGACCAAGGAAAGGTCATTGCCTTCT
CAGGTCGTATCTGGCAAAAAACGGATTCACAAACTTCTAAGTATAAAAACAGTCGATCGACTGCAATTTTTAACAAAAGT
TACGAATTATATCATATGGATAGGGCAAAAAGATCTTCTGGAAAAGCTAGTGAGATTTACCTGATGGAAGGATTCATGGA
TGTTATTGCAGCCTATCGGGCTGGAATCGAAAATGCTGTGGCGTCGATGGGAACGGCCTTGAGTCGAGAGCATGTTGAGC
ATCTGAAAAGGTTAACCAAGAAATTGGTTCTTGTTTACGATGGAGATAAGGCTGGGCAAGCCGCGACATTGAAAGCATTG
GATGAAATTGGTGATATGCCTGTGCAAATCGTTAGCATGCCTGATAACTTGGATCCTGATGAGTATCTACAAAAAAATGG
TCCAGAAGACTTGGCCTATCTATTAACAAAAACTCGTATTAGTCCGATTGAGTTCTACATTCATCAGTACAAACCTGAAA
ACAGTGAAAATCTGCAGGCTCAGATTGAGTTTATTGAAAAAATAGCTCCCTTGATTGTTCAAGAAAAGTCCATCGCTGCT
CAAAACAGCTATATTCATATTTTAGCTGACAGTCTGGCGTCCTTTGATTATACCCAGATTGAGCAGATTGTTAATGAGAG
TCGTCAGGTGCAAAGGCAGAATCGCATGGAAAGAATTTCCAGACCGACGCCAATCACCATGCCTGTCACCAAGCAGTTAT
CGGCTATTATGAGGGCAGAAGCCCATCTACTCTATCGGATGATGGAATCCCCTCTTGTTTTGAACGATTACCGTTTGCGA
GAAGACTTTGCATTTGCTACACCTGAATTTCAGGTCTTATATGACTTGCTTGGCCAGTATGGAAATCTTCCTCCAGAAGT
TTTAGCAGAGCAGACAGAGGAAGTTGAAAGAGCTTGGTACCAAGTTTTAGCTCAGGATTTGCCTGCTGAGATATCGCCGC
AGGAACTTAGTGAAGTAGAGATGACTCGAAACAAGGCTCTCTTGAATCAGGACAATATGAGAATCAAAAAGAAGGTGCAG
GAAGCTAGCCATGTAGGAGATACAGATACAGCCCTAGAAGAATTGGAACGTTTAATTTCCCAAAAGAGAAGAATGGAGTA
A

Upstream 100 bases:

>100_bases
TCTTTGAATAAAGTAAGGAAAATGAGTGAAATGGTTTACTTTTTTTCTGAAATAAAGTATACTATATAAAGTAAACTATG
ATAACATGGAGGTATTGTGT

Downstream 100 bases:

>100_bases
TAATGGCAACAAAACAAAAAGAAGTAACAACATTTGACGTACAGGTAGCAGAATTTATCCGTAATCATAAGCAAAAAGGG
ACAGCAACAGATGATGAAAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKTPSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFI
EAVQILGQRVGIEVEKPLYSEQKSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIWQKTDSQTSKYKNSRSTAIFNKS
YELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGIENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKAL
DEIGDMPVQIVSMPDNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLIVQEKSIAA
QNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVTKQLSAIMRAEAHLLYRMMESPLVLNDYRLR
EDFAFATPEFQVLYDLLGQYGNLPPEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQ
EASHVGDTDTALEELERLISQKRRME

Sequences:

>Translated_586_residues
MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKTPSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFI
EAVQILGQRVGIEVEKPLYSEQKSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIWQKTDSQTSKYKNSRSTAIFNKS
YELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGIENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKAL
DEIGDMPVQIVSMPDNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLIVQEKSIAA
QNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVTKQLSAIMRAEAHLLYRMMESPLVLNDYRLR
EDFAFATPEFQVLYDLLGQYGNLPPEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQ
EASHVGDTDTALEELERLISQKRRME
>Mature_586_residues
MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKTPSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFI
EAVQILGQRVGIEVEKPLYSEQKSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIWQKTDSQTSKYKNSRSTAIFNKS
YELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGIENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKAL
DEIGDMPVQIVSMPDNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLIVQEKSIAA
QNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVTKQLSAIMRAEAHLLYRMMESPLVLNDYRLR
EDFAFATPEFQVLYDLLGQYGNLPPEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQ
EASHVGDTDTALEELERLISQKRRME

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=460, Percent_Identity=31.5217391304348, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 67400; Mature: 67400

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKTPSFNVVEDKQFYHCFG
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEECCCCEEEEEC
CGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQKSASPHQDLYDMHEDAAK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCHHHHCCCCCCHHHHHHHHHHHH
FYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPERNYLYQRLSDQYREEDLLDS
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIWQKTDSQTSKYKNSRSTAIFNKS
CCEEECCHHHHHHHHHCCEEEEEECCCCCEEEECCCEECCCCCHHHHHCCCCCCEEECCC
YELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGIENAVASMGTALSREHVEHLKRLTK
CCEEEHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMPDNLDPDEYLQKNGPEDLAYLLTKTRI
HEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCC
SPIEFYIHQYKPENSENLQAQIEFIEKIAPLIVQEKSIAAQNSYIHILADSLASFDYTQI
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCHHHH
EQIVNESRQVQRQNRMERISRPTPITMPVTKQLSAIMRAEAHLLYRMMESPLVLNDYRLR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHH
EDFAFATPEFQVLYDLLGQYGNLPPEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVE
HHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
MTRNKALLNQDNMRIKKKVQEASHVGDTDTALEELERLISQKRRME
HHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKTPSFNVVEDKQFYHCFG
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEECCCCEEEEEC
CGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQKSASPHQDLYDMHEDAAK
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCHHHHCCCCCCHHHHHHHHHHHH
FYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPERNYLYQRLSDQYREEDLLDS
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIWQKTDSQTSKYKNSRSTAIFNKS
CCEEECCHHHHHHHHHCCEEEEEECCCCCEEEECCCEECCCCCHHHHHCCCCCCEEECCC
YELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGIENAVASMGTALSREHVEHLKRLTK
CCEEEHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMPDNLDPDEYLQKNGPEDLAYLLTKTRI
HEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCC
SPIEFYIHQYKPENSENLQAQIEFIEKIAPLIVQEKSIAAQNSYIHILADSLASFDYTQI
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCHHHH
EQIVNESRQVQRQNRMERISRPTPITMPVTKQLSAIMRAEAHLLYRMMESPLVLNDYRLR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHH
EDFAFATPEFQVLYDLLGQYGNLPPEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVE
HHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
MTRNKALLNQDNMRIKKKVQEASHVGDTDTALEELERLISQKRRME
HHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808 [H]