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The map label for this gene is ruvB [H]

Identifier: 169834021

GI number: 169834021

Start: 321950

End: 322948

Strand: Direct

Name: ruvB [H]

Synonym: SPH_0376

Alternate gene names: 169834021

Gene position: 321950-322948 (Clockwise)

Preceding gene: 169832834

Following gene: 169832824

Centisome position: 14.34

GC content: 45.55

Gene sequence:

>999_bases
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGGCCTCCAGGTCTCGGGAAAACGACCATGGCCTTTGTTATTGCCAACGAACTGGGAGTCAATCTT
AAGCAGACGTCGGGTCCAGTCATTGAAAAAGCCGGAGATCTGGTAGCTATTTTGAATGAGTTAGAGCCTGGGGATGTACT
TTTTATTGATGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATAGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCCAATCCGCTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGGACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTCAATATCGCCGAAGAGCGTGAGACAG
TTGAAGATATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Upstream 100 bases:

>100_bases
CAGTGTCGTAGAATATTATACCACATAAGTGCTAAACTTAGGAGTCACCCAATCAGGTGGCTTTTTTGTTTGTGGCTTGG
TTTTTTGATATAATAGAGCC

Downstream 100 bases:

>100_bases
GCAAGAAATCTTAGAGGCTTTTAGAGAAAATCCAGATATGATGGCCATTCTGACGATCATCCGAGACCTTGGTCTGAAAG
ACTCGTGGTTGGCAGCAGGT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Sequences:

>Translated_332_residues
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK
>Mature_331_residues
SRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLK
QTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRA
GMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVIDD
IITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKA
YEHLGYEYSEK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=319, Percent_Identity=58.9341692789969, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37289; Mature: 37158

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGP
CCCCCCHHCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEECC
PGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVE
CCCCHHHHHHEEHHHHCCEEECCCCCHHHHCCHHHHHHHCCCCCCEEEHHHHHHCCCCHH
EVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLRARFGITGHMEY
HHHHHHHHCEEEEEEEECCCCCCEEEEECCCEEEEECCHHCCHHCCCHHHHCCCCCCHHH
YAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEP
HHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHCC
YLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK
HHHCCCEEEEECCCCEEHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGP
CCCCCHHCCCCHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEECC
PGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVE
CCCCHHHHHHEEHHHHCCEEECCCCCHHHHCCHHHHHHHCCCCCCEEEHHHHHHCCCCHH
EVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLRARFGITGHMEY
HHHHHHHHCEEEEEEEECCCCCCEEEEECCCEEEEECCHHCCHHCCCHHHHCCCCCCHHH
YAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEP
HHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHCC
YLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK
HHHCCCEEEEECCCCEEHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA