Click here to switch to the map view.

The map label for this gene is cls [C]

Identifier: 169833923

GI number: 169833923

Start: 275458

End: 276990

Strand: Direct

Name: cls [C]

Synonym: SPH_0315

Alternate gene names: 169833923

Gene position: 275458-276990 (Clockwise)

Preceding gene: 169833963

Following gene: 169833443

Centisome position: 12.27

GC content: 38.55

Gene sequence:

>1533_bases
ATGAAATATAGGAAATTTCAATTATTGATGTCCAAGTATGGCTTTAGTCTTTCGATTATGCTACTTGAACTTTGTCTTGT
TTTTGGTCTCTTTCTTTATTTAGGACGCATGGCTCCCATTTTATGGATTACTGTCCTCATTCTACTGAGTATCATCACAA
TCATTTCGATAGTCAACCGTAATACGACTCCTGAGAATAAGGTAACCTGGTTGTTAGTAGCCTTTGTGCCAGTATTTGGT
CCCTTGCTCTATCTGATGTTTGGTGAAAGGCGATTGTCCAAAAAAGAAATCAAACAACTGAAGAAGCTAGGCTCTATGCA
TTTCCAAGAAGCAAATAGCCAGCTACTAAAAGAGAAATTAAAAGAAAGTGACAAGGCAGCTTATGGAGTCATCAAGTCCT
TATTGAGTATGGATACCAATGCTGACATCTATGATCAAACTGCCTCTACATTTTTTCCTAACGGAGAAGCTATGTGGAAA
AAGATGGTAGAAGATCTTAAAAAGGCTGAGAAATTTATTTTCTTGGAATATTACATTATAGAAGAAGGTTTGATGTGGAA
TCGCATACTAGATATACTAGAGCAAAAGGTAGCTCAGGGTGTAGAGGTTAAGATGCTCTATGATGATATCGGCTGTATGG
CTACTTTAACAGGAGATTATGCACATCGACTTCGTCAGCTGGGCATCGAGGCCCATAAATTCAATAAAGTTATTCCTCGT
TTGACAGTGGCTTATAATAACAGAGATCATAGAAAAATATTGATTGTTGATGGTCAGATAGCCTATACTGGTGGGGTCAA
TCTGGCAGATGAGTACATTAACCACGTCGAGAGATTTGGTTATTGGAAGGATAGTGGAATTCGCTTAGACGGACTAGCAG
TAAAAGCCCTGACACGCTTATTTTTGACCACTTGGTACATTAATCGAGGAGAAATTAGTGATTTTGATCAATATCATTTA
GAAAATCATTCTATCCCGAGTGACGGTTTAACCATTCCATACGGAAGTGGACCCAAGCCAATTTTTCGAGCGCAGGTAGG
GAAAAAAGTTTATCAGAGTTTAATCAATCAAGCAACGGAATCGGTCTATATTACGACACCTTATTTGATTATAGATTATG
ATTTAACAGAGACAATCAAAAATGCAGCTATGAGAGGGGTCGATGTTCGAATTATCACCCCTTACATACCAGATAAGAAG
TTCATTCAGTTAGTCACGAGAGGAGCTTATCCCGACCTTCTTTCTGCTGGTGTTCGGATTTATGAGTATAGTCCAGGTTT
TATTCATAGTAAGCAGATGTTGGTAGACGAAGATTTTGCGGTGGTGGGGACAATCAATCTCGACTACCGGAGCTTGGTAC
ACCATTATGAAAATGCAGTCTTACTCTATAAAACTCCTTCTATAAGGGAAATCGCCCGAGATTTTCGAAATATATTTGCA
GATTCTCAGGAAGTCTATCCTCATTCTATCAAAACGAGCTGGTATCAAAAGCTTGTAAAAGAAATCGCCCAGCTATTCGC
CCCTATCTTATAA

Upstream 100 bases:

>100_bases
TACCTAGAAAATAATCATAAATACTTGCCTTCTACCGAATACCTGCGTTATACTAGTATCAATTACTTGTTTTTAGCATT
CAAACTATCAAGGAGGGGAT

Downstream 100 bases:

>100_bases
GAAATCTAGAACTTAGGATACAAACCAGACCTATTTTTCTTGTCTTTTACGAATAGAAAGAAAGAGGAGGGGAATTCATG
CTAACTAAGGAAGAAGTGAA

Product: cardiolipin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWITVLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFG
PLLYLMFGERRLSKKEIKQLKKLGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTGDYAHRLRQLGIEAHKFNKVIPR
LTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHVERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHL
ENHSIPSDGLTIPYGSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRIITPYIPDKK
FIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYRSLVHHYENAVLLYKTPSIREIARDFRNIFA
DSQEVYPHSIKTSWYQKLVKEIAQLFAPIL

Sequences:

>Translated_510_residues
MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWITVLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFG
PLLYLMFGERRLSKKEIKQLKKLGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTGDYAHRLRQLGIEAHKFNKVIPR
LTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHVERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHL
ENHSIPSDGLTIPYGSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRIITPYIPDKK
FIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYRSLVHHYENAVLLYKTPSIREIARDFRNIFA
DSQEVYPHSIKTSWYQKLVKEIAQLFAPIL
>Mature_510_residues
MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWITVLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFG
PLLYLMFGERRLSKKEIKQLKKLGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTGDYAHRLRQLGIEAHKFNKVIPR
LTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHVERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHL
ENHSIPSDGLTIPYGSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRIITPYIPDKK
FIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYRSLVHHYENAVLLYKTPSIREIARDFRNIFA
DSQEVYPHSIKTSWYQKLVKEIAQLFAPIL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=452, Percent_Identity=28.3185840707965, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787007, Length=340, Percent_Identity=26.4705882352941, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 58750; Mature: 58750

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWITVLILLSIITIISIVNR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKKLGSMHFQEANSQLLKEKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWKKMVEDLKKAEKFIFLEYYII
HHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEH
EEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTGDYAHRLRQLGIEAHKFNKVIPR
HCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHEEECHHHHHHHHHHCCHHHHHHHHHCC
LTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHVERFGYWKDSGIRLDGLAVKALTRL
EEEEECCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHH
FLTTWYINRGEISDFDQYHLENHSIPSDGLTIPYGSGPKPIFRAQVGKKVYQSLINQATE
HHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
SVYITTPYLIIDYDLTETIKNAAMRGVDVRIITPYIPDKKFIQLVTRGAYPDLLSAGVRI
CEEEECCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHCCCEE
YEYSPGFIHSKQMLVDEDFAVVGTINLDYRSLVHHYENAVLLYKTPSIREIARDFRNIFA
EECCCCCEECCHHEECCCEEEEEEECCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHC
DSQEVYPHSIKTSWYQKLVKEIAQLFAPIL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWITVLILLSIITIISIVNR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKKLGSMHFQEANSQLLKEKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWKKMVEDLKKAEKFIFLEYYII
HHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEEH
EEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTGDYAHRLRQLGIEAHKFNKVIPR
HCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHEEECHHHHHHHHHHCCHHHHHHHHHCC
LTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHVERFGYWKDSGIRLDGLAVKALTRL
EEEEECCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHH
FLTTWYINRGEISDFDQYHLENHSIPSDGLTIPYGSGPKPIFRAQVGKKVYQSLINQATE
HHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
SVYITTPYLIIDYDLTETIKNAAMRGVDVRIITPYIPDKKFIQLVTRGAYPDLLSAGVRI
CEEEECCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHCCCEE
YEYSPGFIHSKQMLVDEDFAVVGTINLDYRSLVHHYENAVLLYKTPSIREIARDFRNIFA
EECCCCCEECCHHEECCCEEEEEEECCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHC
DSQEVYPHSIKTSWYQKLVKEIAQLFAPIL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]