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The map label for this gene is thiM [H]

Identifier: 169833850

GI number: 169833850

Start: 751165

End: 751947

Strand: Direct

Name: thiM [H]

Synonym: SPH_0804

Alternate gene names: 169833850

Gene position: 751165-751947 (Clockwise)

Preceding gene: 169832635

Following gene: 169833352

Centisome position: 33.45

GC content: 36.65

Gene sequence:

>783_bases
ATGACAAGTTTAAAATTATTAAAAGAAAAAGCACCATTGGTCATTTGTATAACCAATGATGTAGTAAAAAATTTCACAGC
AAATGGATTAGTAGCACTGGGCGCCTCACCAGCCATGAGTGAGTTTCCAGCAGATTTAGAGGATTTGTTAAAGTATGCTG
GTGGTTTATTAATAAACATAGGAACATTGACAGATGAAAATTGGAAATTATACCAAGCTGCTCTGAAAATTGCGGAGAAA
TATAATGTCCCAGCAGTTTTAGATCCTGTAGCCTGTGGAGCAGGAGAATATAGAAAAAAAGTAGCAGATGATCTAATCAA
CAATTATAAACTAGCAGCGATTAGAGGAAATGCTGGCGAGATTGCCTCTTTAGTAGGAATAGATGTGGCATCTAAAGGAG
TAGATAGTGCGGGCGTAGATAATATTGACGAAATTGCTCTAGCAGCAAATGAGAAGTTCAATATTCCAATAGTAGTAACA
GGTGAAGTGGATGCCATTGCTGTTAATGGAGAAGTGGTAACGATTCATAATGGTAGTGCTATGATGCCGAAAGTCATTGG
GACAGGATGCTTATTAGGAGCTGTAGTAGCAAGCTTTATCGGACTAGAAAAAGGTCAAGAATTGAAATCATTAGAAACAG
CAATGTTGGTTTACAATATCGCTGGAGAAATAGCAGAAAAACATCCAAATGGACATCTTCCTGGGACATTTAAAGTTGAA
TTTATAAATGCCTTATACGAGATTACAGATGAAGATGTAAAGGAATTCAAAAGAGTGAAGTAA

Upstream 100 bases:

>100_bases
GAGTATAAATCATTCAATGTAAATGAAAAAAATTCTTAACGAAAGATAGAAATAAATCGAAATCAGTAGATCGTATAAAG
TGAAAAGGAAGGATTTCAAT

Downstream 100 bases:

>100_bases
GATGTTTCATAAAGAATTACTAAAACTATATTTTATTTGTGGAACGACTACTTGCCAAGGAAAAAATCTATATACAGTCG
TTGAGGAAGCCTTAAAAGGT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1 [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEK
YNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEIAEKHPNGHLPGTFKVE
FINALYEITDEDVKEFKRVK

Sequences:

>Translated_260_residues
MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEK
YNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEIAEKHPNGHLPGTFKVE
FINALYEITDEDVKEFKRVK
>Mature_259_residues
TSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKY
NVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTG
EVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEIAEKHPNGHLPGTFKVEF
INALYEITDEDVKEFKRVK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=251, Percent_Identity=41.8326693227092, Blast_Score=171, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325042, Length=272, Percent_Identity=33.8235294117647, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 27570; Mature: 27439

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINI
CCCHHHHHCCCCEEEEEECHHHHHCCCCCEEEECCCCCHHHCCCCHHHHHHHCCCEEEEE
GTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGE
ECCCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCHHHHHHHHHHHHCCEEEEEEECCCHH
IASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTGEVDAIAVNGEVVTIHNGSA
HHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCCEEEECCCEEEEECCEEEEEECCCC
MMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEIAEKHPNGHLPGTFKVE
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHH
FINALYEITDEDVKEFKRVK
HHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
TSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINI
CCHHHHHCCCCEEEEEECHHHHHCCCCCEEEECCCCCHHHCCCCHHHHHHHCCCEEEEE
GTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGE
ECCCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCHHHHHHHHHHHHCCEEEEEEECCCHH
IASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTGEVDAIAVNGEVVTIHNGSA
HHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCCEEEECCCEEEEECCEEEEEECCCC
MMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEIAEKHPNGHLPGTFKVE
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHH
FINALYEITDEDVKEFKRVK
HHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA