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The map label for this gene is trmFO [H]

Identifier: 169833706

GI number: 169833706

Start: 967418

End: 968752

Strand: Direct

Name: trmFO [H]

Synonym: SPH_1047

Alternate gene names: 169833706

Gene position: 967418-968752 (Clockwise)

Preceding gene: 169834140

Following gene: 169832939

Centisome position: 43.08

GC content: 45.17

Gene sequence:

>1335_bases
GTGTCTCAATCTTATATCAATGTTATCGGTGCTGGTTTGGCAGGTTCTGAAGCAGCTTACCAAATCGCAGAGCGTGGTAT
TCCAGTTAAACTATATGAAATGCGTGGTGTCAAGTCTACACCCCAGCATAAAACAGACAATTTTGCTGAGTTGGTTTGTT
CCAATTCTTTGCGTGGGGATGCTTTGACAAATGCAGTTGGTCTTCTCAAGGAAGAAATGCGTCGCTTGGGTTCTGTTATC
TTGGAATCTGCTGAGGCTACACGTGTTCCTGCCGGTGGAGCCCTTGCGGTGGATCGTGATGGTTTCTCTCAAATGGTGAC
CGAAAAAGTTGCCAACCACCCCTTGATTGAAGTGGTTCGTGATGAAATTACAGAATTGCCGACAGATGTTATTACGGTTA
TCGCTACTGGTCCTTTGACAAGTGATGCCTTGGCTGAAAAGATTCATGCCCTTAATGACGGTGATGGTTTTTATTTCTAC
GATGCGGCAGCGCCTATTATCGATGTCAACACTATCGATATGAGCAAGGTCTACCTCAAATCACGTTATGATAAGGGAGA
AGCGGCCTACCTCAATGCCCCTATGACCAAGCAAGAATTTATGGATTTCCATGAAGCTTTGGTCAATGCAGAAGAAGCAC
CGCTTAATTCTTTTGAAAAAGAAAAGTACTTTGAAGGATGTATGCCTATCGAAGTTATGGCCAAACGTGGCATTAAAACT
ATGCTTTATGGCCCTATGAAGCCAGTCGGTCTTGAGTATCCGGACGACTACACAGGACCTCGTGATGGAGAATTTAAAAC
ACCTTATGCGGTTGTGCAACTTCGTCAGGATAATGCAGCTGGTAGCCTCTACAATATCGTTGGTTTCCAGACCCACCTCA
AATGGGGAGAACAAAAGCGTGTCTTCCAAATGATTCCGGGTCTTGAAAATGCGGAGTTTGTCCGTTATGGTGTCATGCAT
CGCAATTCTTACATGGATTCACCAAATCTTCTTGAGCAGACTTACCGTTCCAAGAAACAACCAAATCTCTTCTTTGCTGG
TCAGATGACGGGGGTGGAAGGCTATGTTGAGTCGGCGGCTTCGGGCTTAGTTGCGGGAATTAACGCAGCTCGTCTCTTCA
AGGAAGAAAGCGAGGTTATTTTCCCCGAGACGACAGCGATTGGAAGCTTAGCTCATTACATTACCCATGCCGACAGCAAA
CATTTCCAACCAATGAATGTCAATTTTGGGATCATCAAGGAGTTGGAAGGCGAGCGTATCCGTGATAAGAAGGCTCGTTA
TGAAAAAATTGCAGAGCGTGCCCTTGCCGACTTAGAGGAATTTTTGACTGTCTAA

Upstream 100 bases:

>100_bases
TACTAGTAGAAGAAAAAATTAGAAAGATTTGTGGGTGTTAAACAAGCCAGTGCCTTGTTTTGATATGAACTTAGGTTCCA
CCCAAAGAGGTATTAGTGTC

Downstream 100 bases:

>100_bases
TTTTTTTGAAAGAATTGCTCATGATACTATAAAAATCTTAGAAATTGTGATAAAATAGGTAGGATGAAAGAAGGAGAGTG
AAAATGGCGAATCCCAAGTA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVI
LESAEATRVPAGGALAVDRDGFSQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAHYITHADSK
HFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV

Sequences:

>Translated_444_residues
MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVI
LESAEATRVPAGGALAVDRDGFSQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAHYITHADSK
HFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV
>Mature_443_residues
SQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVIL
ESAEATRVPAGGALAVDRDGFSQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFYD
AAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTM
LYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHR
NSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAHYITHADSKH
FQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=85, Percent_Identity=44.7058823529412, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49288; Mature: 49156

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGD
CCCCHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCH
ALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGFSQMVTEKVANHPLIEVVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHH
DEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFYDAAAPIIDVNTIDMSKVYLK
HHHHHCCHHHEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHH
SRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
HCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCHH
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
VFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
SGLVAGINAARLFKEESEVIFPETTAIGSLAHYITHADSKHFQPMNVNFGIIKELEGERI
HHHHHCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHH
RDKKARYEKIAERALADLEEFLTV
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHKTDNFAELVCSNSLRGD
CCCHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCH
ALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGFSQMVTEKVANHPLIEVVR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHH
DEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFYDAAAPIIDVNTIDMSKVYLK
HHHHHCCHHHEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHH
SRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKT
HCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCHH
MLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
VFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQTYRSKKQPNLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
SGLVAGINAARLFKEESEVIFPETTAIGSLAHYITHADSKHFQPMNVNFGIIKELEGERI
HHHHHCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHH
RDKKARYEKIAERALADLEEFLTV
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA