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The map label for this gene is purD [H]

Identifier: 169833688

GI number: 169833688

Start: 133803

End: 135065

Strand: Direct

Name: purD [H]

Synonym: SPH_0158

Alternate gene names: 169833688

Gene position: 133803-135065 (Clockwise)

Preceding gene: 169832675

Following gene: 169834144

Centisome position: 5.96

GC content: 47.66

Gene sequence:

>1263_bases
ATGAAACTGCTTGTTGTCGGTTCGGGTGGTCGTGAGCATGCGATTGCTAAAAAGTTACTTGAATCAAAAGATGTGGAAAA
AGTCTTTGTAGCTCCTGGGAATGATGGGATGACTCTGGATGGTTTGGAATTGGTAAATATCTCTATTTCCGAACATTCTA
AATTGATTGACTTCGCAAAGACCAATGATGTTGCTTGGACCTTTATCGGTCCAGATGATGCCCTTGCTGCTGGTATCGTG
GATGATTTTAACCAAGCTGGGCTTAAGACCTTTGGTCCGACTAGGGCTGCAGCGGAGCTGGAGTGGTCCAAGGATTTTGC
CAAGGAAATCATGGTCAAATATGGTGTTTCGACAGCAGCTTATGGCACATTTTCAGATTTCGAGGAAGCAAAGGCCTATA
TCGAAAAGCATGGTGCGCCTATCGTAGTCAAGGCGGATGGCCTGGCGCTTGGGAAGGGTGTCGTCGTTGCTGAGACGGTT
GAGCAAGCGGTCGAAGCCGCTCATGAGATGCTTTTGGACAATAAATTTGGTGACTCAGGTGCGCGCGTGGTTATTGAGGA
ATTCCTTGAAGGAGAGGAATTTTCACTCTTTGCCTTTGTCAATGGTGATAAGTTCTACATCATGCCAACGGCTCAGGACC
ACAAACGTGCCTACGATGGCGACAAAGGGCCTAACACGGGTGGTATGGGTGCCTATGCGCCAGTTCCACACTTACCACAG
AGTGTAGTTGATACAGCGGTTGACACCATTGTCAAGCCAGTTCTAGAAGGGATGATTAAAGAAGGTCGCCCTTATCTGGG
AGTTCTTTACGCAGGGCTTATCCTGACAGCTGATGGACCGAAAGTCATTGAGTTCAACGCTCGGTTCGGAGATCCAGAAA
CTCAGATTATCTTGCCTCGCTTGACCTCTGACTTTGCTCAAAATATCACAGATATCCTGGATAGCAAGGAGCCAAATATC
ATGTGGACGGACAAGGGTGTGACTCTGGGTGTGGTTGTCGCATCCAAGGGCTACCCGCTAGACTATGAAAGGGGCGTTGA
GTTGCCAGCCAAGACAGAAGGCGATGTCATCACCTACTATGCAGGGGCTAAGTTTGCGGAAAATAGCAGAGCACTGCTCT
CAAACGGCGGACGAGTTTATATGCTCGTTACCACAGCAGATACCGTCAAAGAAGCCCAAGCCAGCATATACCAAGAACTA
TACCAACAAAAAATAGAAGGACTCTTCTACCGAACAGATATCGGAAGCAAGGCAATTAAGTAA

Upstream 100 bases:

>100_bases
AGTTTCTTTCCTTTTTTGGCTTAAAATGCTAACTGAAACAAGATTAAAACGAACTTTTTTGATATAATGTTGGTAAATAA
TTCGCAAAAGAGGTTGAGGA

Downstream 100 bases:

>100_bases
AGATATAAGAATAACGCGCCGTAGTCGCCAAACACGATAATGGTCGTCGTGGTGAAAAGACCAGAACAGTGAATGTTCTG
GTCAGGGGGAAACTTGGAGA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIV
DDFNQAGLKTFGPTRAAAELEWSKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQ
SVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILTADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNI
MWTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQEL
YQQKIEGLFYRTDIGSKAIK

Sequences:

>Translated_420_residues
MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIV
DDFNQAGLKTFGPTRAAAELEWSKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQ
SVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILTADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNI
MWTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQEL
YQQKIEGLFYRTDIGSKAIK
>Mature_420_residues
MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIV
DDFNQAGLKTFGPTRAAAELEWSKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQ
SVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILTADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNI
MWTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQEL
YQQKIEGLFYRTDIGSKAIK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=44, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=44, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=44, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=44, Blast_Score=355, Evalue=6e-98,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=47.0725995316159, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=38.5882352941176, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=38.5135135135135, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=41.7633410672854, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=41.7633410672854, Blast_Score=300, Evalue=8e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45441; Mature: 45441

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAK
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEECCEEEEEEEHHCCCHHEEEEC
TNDVAWTFIGPDDALAAGIVDDFNQAGLKTFGPTRAAAELEWSKDFAKEIMVKYGVSTAA
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCHHC
YGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETVEQAVEAAHEMLLDNKFGDSG
CCCHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCC
ARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQ
HHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILTADGPKVIEFNARFGDPETQIILPR
HHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECEEEECCCCEEEEEECCCCCCCCEEEEHH
LTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVITYY
HHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCHHCCCCCCCCCCCCEEEEE
AGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQELYQQKIEGLFYRTDIGSKAIK
CCCEECCCCHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
>Mature Secondary Structure
MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAK
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEECCEEEEEEEHHCCCHHEEEEC
TNDVAWTFIGPDDALAAGIVDDFNQAGLKTFGPTRAAAELEWSKDFAKEIMVKYGVSTAA
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCHHC
YGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETVEQAVEAAHEMLLDNKFGDSG
CCCHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCC
ARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQ
HHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILTADGPKVIEFNARFGDPETQIILPR
HHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECEEEECCCCEEEEEECCCCCCCCEEEEHH
LTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVITYY
HHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCCCHHCCCCCCCCCCCCEEEEE
AGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQELYQQKIEGLFYRTDIGSKAIK
CCCEECCCCHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]