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The map label for this gene is gidA [H]

Identifier: 169833429

GI number: 169833429

Start: 205453

End: 207366

Strand: Direct

Name: gidA [H]

Synonym: SPH_0236

Alternate gene names: 169833429

Gene position: 205453-207366 (Clockwise)

Preceding gene: 169832731

Following gene: 169832717

Centisome position: 9.15

GC content: 45.98

Gene sequence:

>1914_bases
ATGATTTATCATTTTACTGAAGAATACGATATTATTGTAATTGGTGCGGGACACGCTGGGGTTGAGGCTTCCTTAGCTGC
TAGCCGTATGGGCTGCAAGGTCTTGCTTGCGACCATCAATATTGAAATGCTGGCTTTCATGCCTTGTAATCCCTCTATCG
GTGGTTCTGCTAAGGGGATTGTCGTACGTGAAGTCGATGCCCTCGGTGGCGAGATGGCCAAGACTATTGACAAGACTTAC
ATCCAGATGAAGATGCTCAACACAGGAAAGGGTCCTGCCGTTCGTGCCCTTCGTGCGCAGGCTGATAAGGAACTTTACTC
TAAGGAAATGCGCAAGACAGTTGAAAATCAAGAAAATCTGACCCTTCGTCAAACCATGATTGATGAGATTTTGGTGGAAG
ATGGCAAGGTTGTCGGTGTTCGTACAGCGACTCATCAAGAGTATGCTGCTAAGGCTGTTATTGTGACGACAGGGACTGCT
CTCCGTGGGGAAATTATCATCGGAGACCTCAAGTACTCATCAGGTCCTAACCACAGCTTGGCTTCTATTAACCTAGCTGA
CAATCTCAAGGAACTGGGTCTCGAAATCGGTCGTTTCAAGACAGGAACCCCTCCACGTGTCAAGGCCTCTTCTATCAACT
ACGACGTGACAGAAATCCAACCAGGAGATGAAGCGCCTAATCACTTCTCATACACTTCACGTGATGAGGATTATGTCAAG
GACCAAGTACCATGCTGGTTGACCTATACCAATGGTACCAGTCATGAGATTATCCAAAACAACCTCCACCGTGCGCCTAT
GTTTACAGGTGTGGTCAAGGGAGTGGGACCTCGTTACTGTCCATCGATTGAGGACAAGATTGTGCGCTTTGCGGACAAGG
AACGTCACCAACTCTTCCTTGAGCCAGAGGGACGCAATACAGAGGAAGTCTATGTTCAAGGACTTTCAACCAGTCTGCCT
GAGGATGTCCAACGTGACTTGGTTCATTCCATCAAAGGTCTGGAAAATGCAGAGATGATGCGAACAGGTTATGCCATTGA
GTACGACATGGTCTTGCCTCATCAGTTGCGTGCGACTTTGGAAACCAAGAAAATCTCAGGTCTCTTCACTGCTGGTCAGA
CAAATGGAACATCAGGTTATGAAGAAGCTGCTGGCCAAGGGATTATCGCGGGTATCAATGCGGCTCTGAAAATCCAAGGT
AAACCTGAGTTGATTTTGAAGCGCAGTGATGGTTATATAGGGGTGATGATTGATGACTTAGTGACCAAGGGAACCATTGA
ACCTTACCGTCTCTTGACCAGTCGTGCTGAATACCGTCTCATTCTTCGCCATGACAATGCCGATATGCGTTTGACAGAGA
TGGGCCGTGAGATTGGCCTTGTGGATGATGAACGCTGGACTCGTTTTGAAATCAAGAAAAATCAATTTGATAATGAGATG
AAACGCCTAGACAGTATCAAACTCAAGCCAGTCAAGGAAACCAATGCTAAGGTTGAGGAAATGGGCTTCAAGCCGTTGAC
AGATGCGGTGACAGCCAAGGAATTCCTTCGCCGTCCAGAAGTTTCTTACCAAGATGTGGTGGCCTTCATCGGACCAGCTG
CAGAAGACTTGGATGACAAGATTATCGAATTGATTGAAACAGAAATCAAGTACGAAGGCTATATTTCCAAAGCTATGGAT
CAGGTTGCCAAGATGAAACGCATGGAAGAAAAACGCATTCCAGCTAATATTGACTGGGATGACATCGATTCTATTGCGAC
GGAAGCTCGTCAGAAGTTCAAACTCATCAATCCAGAAACCATCGGCCAAGCCAGCCGTATTTCGGGAGTAAACCCAGCAG
ATATTTCTATTTTGATGGTGTATCTGGAAGGTAAAAATCGTAGTATTTCTAAAACTCTTCAAAAATCAAAATGA

Upstream 100 bases:

>100_bases
GCTCCAGAATATCCAGCTAGTTCCAGCCTTTTTAAAAACAGCCCTACCAGATTGGGAAGGCCAACTAAGACACATTCATC
TTGAGGAATAGGAGAGAAAC

Downstream 100 bases:

>100_bases
TACGTCGTTGGCTTCTTACGAATGAGTTCAAAGCTTGGCTTTGATTCATCTCCAGCCTCCCATAGTTTCCCGAACTATGG
GAGCTAACTCAAGTTATGCT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTY
IQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEAPNHFSYTSRDEDYVK
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLP
EDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQG
KPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWTRFEIKKNQFDNEM
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMD
QVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK

Sequences:

>Translated_637_residues
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTY
IQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEAPNHFSYTSRDEDYVK
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLP
EDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQG
KPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWTRFEIKKNQFDNEM
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMD
QVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK
>Mature_637_residues
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTY
IQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEAPNHFSYTSRDEDYVK
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLP
EDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQG
KPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWTRFEIKKNQFDNEM
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMD
QVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=648, Percent_Identity=40.1234567901235, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=673, Percent_Identity=38.6329866270431, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI183227703, Length=688, Percent_Identity=37.7906976744186, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=619, Percent_Identity=50.0807754442649, Blast_Score=623, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=38.7459807073955, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6321202, Length=627, Percent_Identity=39.7129186602871, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=41.1949685534591, Blast_Score=442, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71061; Mature: 71061

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGI
CEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECEEEEEEECCCCCCCCCCCCE
VVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENL
EEEEHHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTALRGEIIIGDLKYSSGPNHSL
HHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEEEEEECCEEECEEEEEEEECCCCCCCCE
ASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEAPNHFSYTSRDEDYVK
EEEEHHHHHHHHCHHHCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFL
CCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATL
CCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEECHHHHHHHH
ETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQGKPELILKRSDGYIGVMIDDL
HHHHHCEEEECCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEEHHH
VTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWTRFEIKKNQFDNEM
HCCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHCCCCCCCCCCCCCEEEEEEHHHHHHHH
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDK
HHHHCCCCCCCHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
IIELIETEIKYEGYISKAMDQVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCHH
IGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK
CCCHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGI
CEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECEEEEEEECCCCCCCCCCCCE
VVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENL
EEEEHHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTALRGEIIIGDLKYSSGPNHSL
HHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEEEEEECCEEECEEEEEEEECCCCCCCCE
ASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEAPNHFSYTSRDEDYVK
EEEEHHHHHHHHCHHHCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFL
CCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATL
CCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEECHHHHHHHH
ETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQGKPELILKRSDGYIGVMIDDL
HHHHHCEEEECCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEEHHH
VTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWTRFEIKKNQFDNEM
HCCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHCCCCCCCCCCCCCEEEEEEHHHHHHHH
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDK
HHHHCCCCCCCHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
IIELIETEIKYEGYISKAMDQVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCHH
IGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK
CCCHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA