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The map label for this gene is thiE1

Identifier: 169833352

GI number: 169833352

Start: 751949

End: 752578

Strand: Direct

Name: thiE1

Synonym: SPH_0805

Alternate gene names: 169833352

Gene position: 751949-752578 (Clockwise)

Preceding gene: 169833850

Following gene: 169834143

Centisome position: 33.49

GC content: 33.49

Gene sequence:

>630_bases
ATGTTTCATAAAGAATTACTAAAACTATATTTTATTTGTGGAACGACTACTTGCCAAGGAAAAAATCTATATACAGTCGT
TGAGGAAGCCTTAAAAGGTGGTATAACCTTATTTCAATTTCGAGAAAAAGGTGAGGGTGCTTTAGAAGGCTTAGAAAAAC
TTGAGCTAGCTATCCAAATTAAAGAACTTTGTAAAAAATACAATGTTCCATTTATCGTTAATGACGATATAGATTTAGCA
ATGGAAATTGACGCCGATGGCGTACATGTAGGTCAAGATGATATTGGTGTTGATGAAATTAGAAAATTGATGCCAGATAA
AATAATTGGTCTTTCTATAAGAAACGAAGAAGAATTTCAACAATCAAAAGTTGAATATGTAGATTATGTAGGTGTTGGGC
CTGTATTTGGTACCCAGTCAAAGGATGATGCTGGTGGTACTATAGGTTATGAAGGTCTTGAATTGATGAGAAAACTATTG
CCACAAATGCCTTTAGTTGCAATTGGTGGGATACAGACGAAACATATTAAAGACATTATAAAGACCAATATGGATGGCGT
TTCAATCATTTCGGCAATATCGTATGCAAAAAATATAGAAAAAACTGTTCGGGAAATGAGTGAACAATAG

Upstream 100 bases:

>100_bases
CCAAATGGACATCTTCCTGGGACATTTAAAGTTGAATTTATAAATGCCTTATACGAGATTACAGATGAAGATGTAAAGGA
ATTCAAAAGAGTGAAGTAAG

Downstream 100 bases:

>100_bases
ATATCTATCCTTGGATTCTAAGTAAGTAGGATTTTCCTTTTTTAAAAAGCTATGTCCAGATTCAGCTTGAATGGCTAATT
CATCAAACAGGTCATTTGTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase 1; TMP-PPase 1; Thiamine-phosphate synthase 1

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGEGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLA
MEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ

Sequences:

>Translated_209_residues
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGEGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLA
MEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ
>Mature_209_residues
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGEGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLA
MEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=215, Percent_Identity=26.9767441860465, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=229, Percent_Identity=34.9344978165939, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE1_STRPN (Q97RS5)

Other databases:

- EMBL:   AE005672
- PIR:   B95083
- RefSeq:   NP_345219.1
- ProteinModelPortal:   Q97RS5
- SMR:   Q97RS5
- EnsemblBacteria:   EBSTRT00000026756
- GeneID:   930668
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0718
- TIGR:   SP_0718
- GeneTree:   EBGT00050000028192
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   CLSK559895
- BioCyc:   SPNE170187-1:SP_0718-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23276; Mature: 23276

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGEGALEGLEKLELAIQI
CCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHHH
KELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQ
HHHHHHCCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCHHEEEEEECCHHHHH
QSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLLPQMPLVAIGGIQTKHIKDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
KTNMDGVSIISAISYAKNIEKTVREMSEQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKGEGALEGLEKLELAIQI
CCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHHH
KELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRKLMPDKIIGLSIRNEEEFQ
HHHHHHCCCCEEECCCCCEEEEECCCCEEECCCCCCHHHHHHHCCHHEEEEEECCHHHHH
QSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLLPQMPLVAIGGIQTKHIKDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
KTNMDGVSIISAISYAKNIEKTVREMSEQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916