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The map label for this gene is rnhB [H]

Identifier: 169833197

GI number: 169833197

Start: 1181863

End: 1182642

Strand: Direct

Name: rnhB [H]

Synonym: SPH_1274

Alternate gene names: 169833197

Gene position: 1181863-1182642 (Clockwise)

Preceding gene: 169834320

Following gene: 169833048

Centisome position: 52.63

GC content: 41.41

Gene sequence:

>780_bases
ATGGCAACGATTAAAGAAATCAAAGAACTCCTTGTGACAGTCAAGGAGTTAGAAAGCCCTATTTTTTTAGAGCTTGAAAA
GGATAATCGCTCAGGAGTTCAAAAGGAAATCAGCAAGCGTAAAAGAGCCATTCAAGCTGAATTAGATGAAAATTTGCGCT
TGGAATCCATGCTTTCTTATGAAAAAGAACTTTATAAGCAAGGATTGACCTTAATTGCAGGTATTGATGAGGTTGGTCGT
GGTCCTCTTGCTGGTCCTGTAGTCGCTGCGGCCGTTATTTTACCTAAAAATTGTAAGATTAAAGGTCTCAACGACAGCAA
GAAAATTCCTAAAAAGAAACATCTGGAGATTTTCCAAGCCGTTCAAGACCAAGCCTTGTCGATTGGAATTGGTATCATAG
ATAATCAGGTCATCGACCAAGTCAATATCTATGAAGCAACCAAACTAGCCATGCAAGAAGCAATCTCCCAGCTCAGCCCT
CAACCAGAGCACCTTTTGATTGATGCCATGAAACTGGACTTGCCCATTTCACAAACCTCCATTATCAAAGGAGATGCCAA
CTCCCTCTCTATCGCAGCAGCATCTATAGTAGCCAAGGTAACACGTGATGAATTGATGAAGGAATACGATCAGCAGTTCC
CTGGCTATGATTTCGCTACTAATGCAGGATATGGCACAGCTAAACATCTGGAAGGCCTCACAAAACTAGGAGCTACCCCA
ATTCACCGAACCAGCTTTGAACCCGTTAAATCACTGGTTTTAGGTAAAAAAGAAAGTTAA

Upstream 100 bases:

>100_bases
GGTTTCCGAGATGACTATGACCGCTTTTACAGTCTCTTCGTGAAGGAAGTCCGTGATGGCAAACTCGGTAACTATACCTT
AGATACATTGGAAGACCTCG

Downstream 100 bases:

>100_bases
TTGAAAGGAAATACCATGGAGGAACAGTCGGAAATAGTCCGTTCTAAGAAAGAATTCGCCTTTGCATCTAGCACTATACT
ATCCCAAGTTGGTCGAGGAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGR
GPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGATP
IHRTSFEPVKSLVLGKKES

Sequences:

>Translated_259_residues
MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGR
GPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGATP
IHRTSFEPVKSLVLGKKES
>Mature_258_residues
ATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRG
PLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSPQ
PEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGATPI
HRTSFEPVKSLVLGKKES

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=181, Percent_Identity=50.2762430939227, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 28458; Mature: 28327

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSY
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQA
HHHHHHCCCCEEECHHHHCCCCCCHHHHHHHHHCCCCCEEECCCCHHHCCHHHHHHHHHH
VQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSPQPEHLLIDAMKLDLPISQTS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
IIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGATP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IHRTSFEPVKSLVLGKKES
CCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAIQAELDENLRLESMLSY
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKGLNDSKKIPKKKHLEIFQA
HHHHHHCCCCEEECHHHHCCCCCCHHHHHHHHHCCCCCEEECCCCHHHCCHHHHHHHHHH
VQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSPQPEHLLIDAMKLDLPISQTS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
IIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDFATNAGYGTAKHLEGLTKLGATP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IHRTSFEPVKSLVLGKKES
CCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA