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The map label for this gene is murD [H]

Identifier: 169833121

GI number: 169833121

Start: 734054

End: 735406

Strand: Direct

Name: murD [H]

Synonym: SPH_0782

Alternate gene names: 169833121

Gene position: 734054-735406 (Clockwise)

Preceding gene: 169833991

Following gene: 169832394

Centisome position: 32.69

GC content: 44.35

Gene sequence:

>1353_bases
ATGAAAGTAATAGATCAATTTAAAAATAAGAAAGTCCTTGTTTTAGGTTTGGCCAAGTCTGGTGAATCTGCAGCTCGTTT
GTTGGACAAGCTAGGTGCCATTGTGACAGTAAATGATGGGAAACCTTTCGAGGACAATCCAGCTGCCCAAAGTTTGCTGG
AAGAAGGGATCAAGGTCATTACAGGTGGCCATCCTTTGGAACTCTTGGATGAAGAGTTTGCCCTTATGGTGAAAAATCCA
GGTATCCCCTACAACAATCCCATGATTGAAAAGGCTTTGGCCAAGGGAATTCCAGTCTTGACTGAGGTGGAATTGGCTTA
TTTGATTTCAGAAGCACCGATTATTGGTATCACAGGATCGAACGGTAAGACAACCACAACGACTATGATTGGGGAAGTTT
TGACTGCTGCTGGCCAACATGGTCTTTTATCAGGGAATATCGGCTATCCAGCTAGTCAGGTTGCTCAAATAGCATCAGAT
AAGGACACGCTTGTTATGGAACTTTCTTCTTTCCAACTCATGGGTGTTCAAGAATTCCATCCAGAGATTGCGGTTATTAC
CAACCTCATGCCAACTCATATCGACTACCATGGGTCATTTTCGGAATATGTAGCAGCCAAGTGGAATATCCAGAACAAGA
TGACAGCAGCTGATTTCCTTGTCTTGAACTTTAATCAAGACTTGGCAAAAGACTTGACTTCCAAGACAGAAGCCACTGTT
ATACCATTTTCAACACTTGAAAAGGTTGATGGAGCTTATCTGGAAGATGGTCAACTCTACTTCCGTGGTGAAGTAGTCAT
GGCAGCGAATGAAATCGGTGTTCCAGGTAGCCACAATGTGGAAAATGCCCTTGCGACTATTGCTGTAGCCAAGCTTCGTG
ATGTGGACAATCAAACCATCAAGGAAACTCTTTCAGCCTTCGGTGGTGTCAAACACCGTCTCCAGTTTGTGGATGACATC
AAGGGTGTTAAATTCTATAACGACAGTAAATCAACTAATATCTTGGCTACTCAAAAAGCCTTGTCAGGATTTGACAACAG
CAAGGTCGTCTTGATTGCAGGTGGTTTGGACCGTGGCAATGAGTTTGACGAATTGGTGCCAGACATTACTGGACTCAAGA
AGATGGTCATCCTGGGTCAATCTGCAGAACGTGTCAAACGGGCAGCAGACAAGGCTGGTGTCGCTTATGTGGAGGCGACA
GATATTGCAGATGCGACCCGCAAGGCCTATGAGCTTGCGATTCAAGGAGATGTGGTTCTTCTTAGTCCTGCCAATGCTAG
CTGGGATATGTATGCTAACTTTGAAGTACGTGGCGACCTCTTTATCGACACAGTAGCGGAGTTAAAAGAATAA

Upstream 100 bases:

>100_bases
GGTAAGATTCAGTTAGAAGAAAGAGTCACAAACACACTTTGTGGCTTTTTTATTTCCATAAAAATGGTAAAATAGTAGGA
GTAGAAATGGAGTTCGAGAC

Downstream 100 bases:

>100_bases
TATGAAAAAAATTGTCTTTACAGGTGGGGGGACGGTTGGACACGTTACCCTCAATCTTTTGTTAATGCCCAAGTTCATCG
AAGATGGTTGGGAAGTCCAC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNP
GIPYNNPMIEKALAKGIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
IPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDI
KGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEAT
DIADATRKAYELAIQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE

Sequences:

>Translated_450_residues
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNP
GIPYNNPMIEKALAKGIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
IPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDI
KGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEAT
DIADATRKAYELAIQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
>Mature_450_residues
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNP
GIPYNNPMIEKALAKGIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
IPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDI
KGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEAT
DIADATRKAYELAIQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=452, Percent_Identity=31.6371681415929, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48507; Mature: 48507

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVI
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCEEE
TGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTEVELAYLISEAPIIGITGS
ECCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHCCCCEEEEECC
NGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDTLVMELSSFQLMGVQEFH
CCCEEHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEECCCEEECHHHHC
PEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
CCCEEECCCCCCCEECCCCHHHHEEEEECCCCCCEEEEEEEEECCHHHHHHHHCCCCEEE
IPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTI
ECHHHHHHCCCCEEECCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
KETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGN
HHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEATDIADATRKAYELAIQGDVVL
CHHHHCCCHHCCCEEEEECCCHHHHHHHHHHCCEEEEEECCHHHHHHHHEEEEEECCEEE
LSPANASWDMYANFEVRGDLFIDTVAELKE
EECCCCCCEEEEEEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVI
CCCHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCEEE
TGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTEVELAYLISEAPIIGITGS
ECCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHCCCCEEEEECC
NGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDTLVMELSSFQLMGVQEFH
CCCEEHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEECCCEEECHHHHC
PEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATV
CCCEEECCCCCCCEECCCCHHHHEEEEECCCCCCEEEEEEEEECCHHHHHHHHCCCCEEE
IPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTI
ECHHHHHHCCCCEEECCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
KETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGN
HHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCC
EFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEATDIADATRKAYELAIQGDVVL
CHHHHCCCHHCCCEEEEECCCHHHHHHHHHHCCEEEEEECCHHHHHHHHEEEEEECCEEE
LSPANASWDMYANFEVRGDLFIDTVAELKE
EECCCCCCEEEEEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA