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The map label for this gene is clcA [H]

Identifier: 169833048

GI number: 169833048

Start: 1182658

End: 1184208

Strand: Direct

Name: clcA [H]

Synonym: SPH_1275

Alternate gene names: 169833048

Gene position: 1182658-1184208 (Clockwise)

Preceding gene: 169833197

Following gene: 169834019

Centisome position: 52.67

GC content: 41.46

Gene sequence:

>1551_bases
ATGGAGGAACAGTCGGAAATAGTCCGTTCTAAGAAAGAATTCGCCTTTGCATCTAGCACTATACTATCCCAAGTTGGTCG
AGGAATCATTGTCGGCCTCATCGTTGGAATTATCGTCGGATCCTTTCGTTTCTTAATTGAAAAGGGCTTCCACCTGATAC
AAGGAGTTTATCAAGATCAAGGGTACTTAGTGCGCAATCTTTTTGTACTGGTTTTGTTTTATATACTCATCTGTTGGCTC
AGTGCCAAACTAACACGGTCAGAAAAAGATATTAAAGGCTCAGGAATTCCTCAAGTCGAAGCCGAACTGAAAGGCCTCAT
GTCCCTCAACTGGTGGGGCATTCTTTGGAAAAAATATGTGCTAGGTATTCTTGCTATTGCCAGTGGACTCATGCTGGGTC
GAGAGGGACCCAGCATTCAACTTGGAGCAGTTGGTGGTAAAGGAATTGCCAAGTGGCTCAAATCCAGTCCAGTAGAGGAA
CGTTCCTTGATTGCCAGTGGAGCTGCAGCAGGTTTAGCCGCAGCCTTTAATGCTCCTATTGCAGCACTTCTCTTTGTTGT
AGAAGAAGTCTATCACCATTTTTCGCGCTTTTTCTGGGTCTCAACTCTAGCAGCCAGCATCGTAGCAAACTTTGTGTCTC
TACTCATGTTTGGTTTGACACCAGTATTGGATATGCCAGATAACATTCCTCCCATGACCCTAGATCAGTATTGGATATAT
CTCGTCATGGGAATTTTCCTTGGATTTTTAGGTTTTCTCTATGAGAAAGCTGTATTAAACGTTGGAAGAGTTTATGACTT
GATTGGTCAAAAAATCCATTTGGATAGGGCTTATTATCCCATCTTGGCTTTTATCCTTATCATACCAGTCGGAATCTTCT
TACCTCAAATCATTGGTGGCGGAAATCAGCTTGTCCTTTCTTTAACTGAACAAAATTTTAGTTTCCAAGTTTTATTAGCT
TACTTTTTGATCCGCTTTATTTGGAGTATGATTAGCTATGGAAGTGGACTGCCAGGAGGAATTTTCCTCCCCATTTTAGC
TCTTGGTTCTTTGCTTGGTGCCTTAGTTGGTGTTATCTGTGTCAATCTTGGACTTGTCAGTCAAGAGCAATTCCCTATAT
TTGTCATTCTAGGAATGAGTGGCTATTTTGGAGCCATATCAAAAGCTCCCTTAACCGCTATGATCCTCGTAACTGAGATG
GTAGGAGATATTCGCAACCTCATGCCACTTGGTCTTGTCACTCTTGTTTCTTATATTATCATGGATTTGCTCAAAGGTAC
GCCAGTCTATGAAGCCATGCTGGAAAAAATGCTTCCAGAAGAAGTATCTAGCGAAGGAGAAGTTACACTTATCGAAATAC
CAGTTTCTGATAAAATTGCTGGGAAACAAGTTCATGAACTCAACTTACCACACAACGTCCTCATCACAACTCAAGTCCAT
AATGGCAAGAGCCAAACAGTTAACGGCTCAACCAGAATGTATCTGGGTGATATGATTCACCTGGTTATTCCAAAAAGTGA
AATTGGAAAAGTCAAAGATTTGTTGTTGTAG

Upstream 100 bases:

>100_bases
CCTCACAAAACTAGGAGCTACCCCAATTCACCGAACCAGCTTTGAACCCGTTAAATCACTGGTTTTAGGTAAAAAAGAAA
GTTAATTGAAAGGAAATACC

Downstream 100 bases:

>100_bases
TATGAGCATTTACATAACATAAATTATGTAAATGCTCAGCTTGATTTATTTAGAAAACCGATTCTCAGAAATGAGATCGG
TTATTTTTTACTGATGAGGA

Product: voltage-gated chloride channel family protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRFLIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWL
SAKLTRSEKDIKGSGIPQVEAELKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFGLTPVLDMPDNIPPMTLDQYWIY
LVMGIFLGFLGFLYEKAVLNVGRVYDLIGQKIHLDRAYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLA
YFLIRFIWSMISYGSGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLTAMILVTEM
VGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTLIEIPVSDKIAGKQVHELNLPHNVLITTQVH
NGKSQTVNGSTRMYLGDMIHLVIPKSEIGKVKDLLL

Sequences:

>Translated_516_residues
MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRFLIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWL
SAKLTRSEKDIKGSGIPQVEAELKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFGLTPVLDMPDNIPPMTLDQYWIY
LVMGIFLGFLGFLYEKAVLNVGRVYDLIGQKIHLDRAYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLA
YFLIRFIWSMISYGSGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLTAMILVTEM
VGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTLIEIPVSDKIAGKQVHELNLPHNVLITTQVH
NGKSQTVNGSTRMYLGDMIHLVIPKSEIGKVKDLLL
>Mature_516_residues
MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRFLIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWL
SAKLTRSEKDIKGSGIPQVEAELKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFGLTPVLDMPDNIPPMTLDQYWIY
LVMGIFLGFLGFLYEKAVLNVGRVYDLIGQKIHLDRAYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLA
YFLIRFIWSMISYGSGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLTAMILVTEM
VGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTLIEIPVSDKIAGKQVHELNLPHNVLITTQVH
NGKSQTVNGSTRMYLGDMIHLVIPKSEIGKVKDLLL

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI55770838, Length=408, Percent_Identity=24.0196078431373, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI55770840, Length=408, Percent_Identity=24.0196078431373, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI189217923, Length=446, Percent_Identity=22.8699551569507, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI189217921, Length=446, Percent_Identity=22.8699551569507, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4557473, Length=446, Percent_Identity=22.8699551569507, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI119433677, Length=444, Percent_Identity=22.0720720720721, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI153252026, Length=442, Percent_Identity=22.1719457013575, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI156104869, Length=322, Percent_Identity=23.6024844720497, Blast_Score=66, Evalue=8e-11,
Organism=Homo sapiens, GI283806622, Length=322, Percent_Identity=23.6024844720497, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1786350, Length=435, Percent_Identity=29.8850574712644, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71985141, Length=422, Percent_Identity=24.4075829383886, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71985148, Length=422, Percent_Identity=24.4075829383886, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71980820, Length=447, Percent_Identity=23.2662192393736, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71980824, Length=440, Percent_Identity=23.1818181818182, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71980828, Length=440, Percent_Identity=23.1818181818182, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17532615, Length=400, Percent_Identity=23.75, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71996452, Length=413, Percent_Identity=23.2445520581114, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71996455, Length=413, Percent_Identity=23.2445520581114, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322500, Length=354, Percent_Identity=25.9887005649718, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665008, Length=428, Percent_Identity=24.2990654205607, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45551566, Length=380, Percent_Identity=26.5789473684211, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 56500; Mature: 56500

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCHHHHHHHHHEEECCHHHHHHHHHCC
>Mature Secondary Structure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CCCCCCCCCCCHHHHHHHHHEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA