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The map label for this gene is ascB [H]

Identifier: 169833005

GI number: 169833005

Start: 778100

End: 778381

Strand: Direct

Name: ascB [H]

Synonym: SPH_0836

Alternate gene names: 169833005

Gene position: 778100-778381 (Clockwise)

Preceding gene: 169834189

Following gene: 169833496

Centisome position: 34.65

GC content: 44.33

Gene sequence:

>282_bases
GTGGATACACCGGATGAGAATGGTTATGTAGCAGATGACTATCGGATTACTTACTTAGAGGCCCACATCAAGGCCATGCG
AGATGCCATTTACAAAGACGGGGTTGACTTGCTTGGTTATACGACATGGGGCTGTATCGATCCGGTTTCAGCTGGCACAG
GGGAAATGAATAAGCGCTATGGCTTTATCTATGTAGATCGAGATAATGTAGGAAACGGAGCTCTCAAACGTAGCAAGAAG
AAGTCCTTCTATTGGTACATGTCATTTATAGCAATGGTGTAA

Upstream 100 bases:

>100_bases
TTTGATGGTTTGGATTTCTTCTTTAGTTGATTTCATATCACTATTATATAATGCTTTTTGATTTTAGTATAGTATTATCG
TAGAAAATGGACTCGGTGCT

Downstream 100 bases:

>100_bases
GCATCGAGTAGAGCACATTTGTTTACTATTTTTATAAAATCTTCTCCCTGCTTTATAAGATGTAAAAAAGAGTTCAAATG
GAACTCTTTTTTTGCTATAA

Product: 6-phospho-beta-glucosidase AscB, truncation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 93; Mature: 93

Protein sequence:

>93_residues
MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKK
KSFYWYMSFIAMV

Sequences:

>Translated_93_residues
MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKK
KSFYWYMSFIAMV
>Mature_93_residues
MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKK
KSFYWYMSFIAMV

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789070, Length=85, Percent_Identity=65.8823529411765, Blast_Score=122, Evalue=4e-30,
Organism=Escherichia coli, GI2367174, Length=90, Percent_Identity=57.7777777777778, Blast_Score=109, Evalue=3e-26,
Organism=Escherichia coli, GI2367270, Length=89, Percent_Identity=55.0561797752809, Blast_Score=102, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 10655; Mature: 10655

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDPVSAGTGEMNKRY
CCCCCCCCEEECCEEEEHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCE
GFIYVDRDNVGNGALKRSKKKSFYWYMSFIAMV
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDPVSAGTGEMNKRY
CCCCCCCCEEECCEEEEHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCE
GFIYVDRDNVGNGALKRSKKKSFYWYMSFIAMV
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]