Click here to switch to the map view.

The map label for this gene is hup [H]

Identifier: 169832956

GI number: 169832956

Start: 1101950

End: 1102225

Strand: Direct

Name: hup [H]

Synonym: SPH_1205

Alternate gene names: 169832956

Gene position: 1101950-1102225 (Clockwise)

Preceding gene: 169834516

Following gene: 169832773

Centisome position: 49.07

GC content: 40.58

Gene sequence:

>276_bases
ATGGCAAACAAACAAGATTTGATCGCTAAAGTAGCAGAAGCTACAGAATTGACTAAGAAAGACTCAGCAGCAGCAGTTGA
AGCTGTATTTGTAGCAGTAGCTGACTATCTTGCAGCTGGTGAAAAAGTTCAGTTGATCGGTTTTGGTAACTTTGAAGTTC
GTGAGCGTGCAGAACGTAAAGGTCGCAACCCACAAACTGGTAAAGAAATGACAATTGCAGCTTCTAAAGTACCAGCATTC
AAAGCTGGTAAAGCTCTTAAAGACGCTGTTAAATAA

Upstream 100 bases:

>100_bases
AAATAAAATGGGAAGAAATAGCATTTTTAACTTGACCTAAAAGGGATTTTAGGATATGATTATATTTGTTAATTAGAAAT
TATTTGGAGGAATCATTAAC

Downstream 100 bases:

>100_bases
TCAGTCTTTAAAAAGCCTATTGTATCAAGCTTTCTAGCTTGGTCAGTAGGCTTTTTAATTATGAAATTTAGTAATAAATA
GAGTTAAAAAGTTGAATGTT

Product: DNA-binding protein HU 1 (DNA-binding protein II) (HB)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MANKQDLIAKVAEATELTKKDSAAAVEAVFVAVADYLAAGEKVQLIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAF
KAGKALKDAVK

Sequences:

>Translated_91_residues
MANKQDLIAKVAEATELTKKDSAAAVEAVFVAVADYLAAGEKVQLIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAF
KAGKALKDAVK
>Mature_90_residues
ANKQDLIAKVAEATELTKKDSAAAVEAVFVAVADYLAAGEKVQLIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFK
AGKALKDAVK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Seems also to act as a fortuitous virulence factor in delayed sequelae by binding to heparan su

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=58.4269662921348, Blast_Score=105, Evalue=8e-25,
Organism=Escherichia coli, GI1786644, Length=88, Percent_Identity=55.6818181818182, Blast_Score=102, Evalue=6e-24,
Organism=Escherichia coli, GI1788005, Length=75, Percent_Identity=42.6666666666667, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1787141, Length=87, Percent_Identity=37.9310344827586, Blast_Score=67, Evalue=2e-13,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9678; Mature: 9547

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKQDLIAKVAEATELTKKDSAAAVEAVFVAVADYLAAGEKVQLIGFGNFEVRERAERK
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHC
GRNPQTGKEMTIAASKVPAFKAGKALKDAVK
CCCCCCCCEEEEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
ANKQDLIAKVAEATELTKKDSAAAVEAVFVAVADYLAAGEKVQLIGFGNFEVRERAERK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHC
GRNPQTGKEMTIAASKVPAFKAGKALKDAVK
CCCCCCCCEEEEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9423866 [H]