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The map label for this gene is mtbP [H]

Identifier: 169832810

GI number: 169832810

Start: 1142145

End: 1143503

Strand: Direct

Name: mtbP [H]

Synonym: SPH_1233

Alternate gene names: 169832810

Gene position: 1142145-1143503 (Clockwise)

Preceding gene: 169833455

Following gene: 169833211

Centisome position: 50.86

GC content: 39.07

Gene sequence:

>1359_bases
ATGAGATTTATTGATTTATTTTCAGGTATCGGTGGCTTTCGACTTGGAATGGAAAGTGTTGGACACGAGTGTATTGGATT
TTGTGAGATTGATAAATTTGCTAGAGAATCTTATAAATCCATTTTTCAAACGGAAGGAGAAATCGAATTTCATGACATAC
GAGATGTTTCAGATGACGAATTTAAAAAACTTAGAGGGAAAGTCGATGTCATCTGTGGGGGATTCCCTTGTCAAGCATTT
TCAATCGCAGGAAGACGATTGGGATTTGAAGATACTAGAGGAACTTTGTTCTTTGAAATTGCTCGGGCGGCCAAACAAAT
CCAACCACGTTTTCTTTTTCTTGAAAATGTTAAAGGCCTACTCAATCACGATAAGGGACGGACGTTCACCACAATACTTA
CCACACTTGATGAGTTGGGGTTTGATGTTGAGTGGCAGGTGCTTAACAGTAAGGATTTTGGCGTTCCCCAAAACAGAGAG
AGGGTGTTTATTATCGGACATTCTAGAAAGAAAGGTACCAGACTCCTATTTCCTTTCAGACGAGAAGGTCAAGCAACTAA
CTCTGAGACTTTAAAAATATTAGGAAATTTGAATCCATCAAAAAGTGGAATGAGCGGTAAAGTCTATTATTCAGAAGGTC
TTGCGCCAACCTTAGTTCGTAGTAAAGGAGAAGGATTTAAAGTTGCGATTCCTTGTATGACACCAGATAGATTAGACAAA
AGACAAAATAGTAGACGCTTTAAGGAAAATCAAGGGCCGATGTTTACTTTAAATACTCAGGATCGTCATGGCATTGTCGT
TGTTGGAGATTTACCGACTAGCTTTAAGGAGACAGGTCGTGTCTATGGAAGTGAGGGCTTATCTCCAACACTGACTACAA
TGCAAGGTGGAGATAAAATCCCCAAAATACTAATTCCAGAACCAATCCAATTTCTAAAAGTCCGAGAGGCAACTAAAAAA
GGATATGCTCAAGCAGAAATAGGAGATTCAATCAATTTGGAGAGACCAAGTTCTCAGTATCGTCGTGGTAGAGTTGGAAA
AGGTATAGCAAATACGTTAACAACCAGTGGGCAAATGGGAGTAGTAGTGGCTAGCTATGAAGGCGAAGATAAACAAGTTT
ATCATGTAGCCGGTGTCTTAATAGATGGACAATTTTACCGTTTGAGAATACGACGAATCACTCCTAAAGAGTGTTTTCGA
TTGCAGGGGTTTCCTGATTGGGCTTTTGAAGCTGCGAGAAAAGTCTCTAGTAATAGTCAGCTCTATAAACAAGCTGGTAA
TAGTGTAACCGTTCCTGTGATTGCCGCAATCGCAAATAAATTAAAAGAAATAGAGGAAAAAGATGAAAGCATTAAATAA

Upstream 100 bases:

>100_bases
AAATGTCGCCAATCGAGTGGATATTAGTTATGCTCATTGTCATTTCAAGTGGAGTGACAGTTCTGACAATGATAGTCGAT
AAGAAAGGGGTGATAAAGAA

Downstream 100 bases:

>100_bases
AGAATCAATACTAGATTGTGATGAATTAGAAACAGAATTACATGATGCAGAAATCAAACAGCTGGATGAACAAATATTTT
TGATGCCCAATTATCCATGT

Product: methyl transferase

Products: NA

Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAF
SIAGRRLGFEDTRGTLFFEIARAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
RVFIIGHSRKKGTRLLFPFRREGQATNSETLKILGNLNPSKSGMSGKVYYSEGLAPTLVRSKGEGFKVAIPCMTPDRLDK
RQNSRRFKENQGPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKK
GYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRRITPKECFR
LQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIANKLKEIEEKDESIK

Sequences:

>Translated_452_residues
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAF
SIAGRRLGFEDTRGTLFFEIARAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
RVFIIGHSRKKGTRLLFPFRREGQATNSETLKILGNLNPSKSGMSGKVYYSEGLAPTLVRSKGEGFKVAIPCMTPDRLDK
RQNSRRFKENQGPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKK
GYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRRITPKECFR
LQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIANKLKEIEEKDESIK
>Mature_452_residues
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAF
SIAGRRLGFEDTRGTLFFEIARAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
RVFIIGHSRKKGTRLLFPFRREGQATNSETLKILGNLNPSKSGMSGKVYYSEGLAPTLVRSKGEGFKVAIPCMTPDRLDK
RQNSRRFKENQGPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKK
GYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRRITPKECFR
LQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIANKLKEIEEKDESIK

Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788271, Length=230, Percent_Identity=36.9565217391304, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 50696; Mature: 50696

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDE
CCHHHHHCCCCHHHHCHHHCCHHHEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
FKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIARAAKQIQPRFLFLENVKGL
HHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHH
LNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRERVFIIGHSRKKGTRLLFPFR
HCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEEC
REGQATNSETLKILGNLNPSKSGMSGKVYYSEGLAPTLVRSKGEGFKVAIPCMTPDRLDK
CCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCHHHHHCCCCCEEEEEECCCCHHHHH
RQNSRRFKENQGPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKI
HHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHCCEECCCCCCCEEEECCCCCCC
PKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMG
CEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCE
VVVASYEGEDKQVYHVAGVLIDGQFYRLRIRRITPKECFRLQGFPDWAFEAARKVSSNSQ
EEEEECCCCCCEEEEEEEEEECCEEEEEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCHH
LYKQAGNSVTVPVIAAIANKLKEIEEKDESIK
HHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQTEGEIEFHDIRDVSDDE
CCHHHHHCCCCHHHHCHHHCCHHHEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
FKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIARAAKQIQPRFLFLENVKGL
HHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHH
LNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRERVFIIGHSRKKGTRLLFPFR
HCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEEC
REGQATNSETLKILGNLNPSKSGMSGKVYYSEGLAPTLVRSKGEGFKVAIPCMTPDRLDK
CCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCHHHHHCCCCCEEEEEECCCCHHHHH
RQNSRRFKENQGPMFTLNTQDRHGIVVVGDLPTSFKETGRVYGSEGLSPTLTTMQGGDKI
HHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHCCEECCCCCCCEEEECCCCCCC
PKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPSSQYRRGRVGKGIANTLTTSGQMG
CEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCE
VVVASYEGEDKQVYHVAGVLIDGQFYRLRIRRITPKECFRLQGFPDWAFEAARKVSSNSQ
EEEEECCCCCCEEEEEEEEEECCEEEEEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCHH
LYKQAGNSVTVPVIAAIANKLKEIEEKDESIK
HHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]