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The map label for this gene is speE [H]

Identifier: 169832707

GI number: 169832707

Start: 949217

End: 950077

Strand: Direct

Name: speE [H]

Synonym: SPH_1027

Alternate gene names: 169832707

Gene position: 949217-950077 (Clockwise)

Preceding gene: 169832494

Following gene: 169833185

Centisome position: 42.27

GC content: 44.25

Gene sequence:

>861_bases
ATGGATTTATGGTTTTCTGAAGTTCATACTCCAGATGTCAAATTGTCTCTGAGAACAGCCAAGCAACTTTACGCTGGAAA
AAGTGAATGGCAGGATATCGAAGTCTTGGATACGCCAGCTTTTGGGAAAATACTGATTTTAAATGGCCATGTCTTGTTCT
CAGATGCGGATGATTTCGTCTACAATGAAATGACCGTCCACGTTCCCATGGCTGTCCACCCAAATCCAAAGAAAGTATTG
GTTATTGGGGGTGGTGACGGCGGTGTTGCCCAAGTATTAACACTCTATCCTGAACTGGAACAAATCGATATCGTGGAACC
GGATGAGATGTTGGTCGAGGTCTGTCGTGAGTATTTCCCAGACTTTGCTGCAGGGCTAGATGATCCTCGTGTTACCATTT
ACTACCAAAATGGGCTACGCTTTTTGCGAAACTGCGAAGATGATTACGATATTATCATCAACGATGCGACAGATCCATTT
GGCCATACGGAAGGACTCTTTACCAAGGAATTCTACGGCAATAGTTATCGAGCTCTGAAGGAAGACGGCATCATGATTTA
CCAGCATGGGAGTCCCTTCTTTGACGAGGATGAGTCGGCCTGCCGAAGCATGCACCGCAAGGTCAATCAATCCTTTCCAA
TCAGTCGGGTTTATCAGGCCCATATTCCAACTAGCCCAGCTGGCTATTGGTTGTTTGGATTTGCATCGAAAAAATACCAC
CCTGTCAAAGATTTTGACAAGGAAGGTTGGAAAAAACGCCAGCTTTTCACAGAATACTACACTGCAAACTTACACGTGGG
ATCCTTTATGTTGCCCAAGTATGTTGAGGACATTTTAGAAGAAGAGGAAGGAAAAAAATGA

Upstream 100 bases:

>100_bases
GATTGGAGGAAATGAACTAATCCACAGTGACTTATTCCAAGTATACCACTTGGGCTTTGGCAGTAGCTAACTGCGCTAAA
TATAATATAAGGAGAAATAG

Downstream 100 bases:

>100_bases
GTCGTTTACTAGTTATTGGTTGTGGGGGCGTTGCCCAAGTTGCTATTTCAAAGATTTGTCAAGATAGCGAAACATTTACA
GAGATTATGATTGCTAGCCG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVL
VIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVYQAHIPTSPAGYWLFGFASKKYH
PVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVEDILEEEEGKK

Sequences:

>Translated_286_residues
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVL
VIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVYQAHIPTSPAGYWLFGFASKKYH
PVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVEDILEEEEGKK
>Mature_286_residues
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVL
VIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVYQAHIPTSPAGYWLFGFASKKYH
PVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVEDILEEEEGKK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=285, Percent_Identity=32.280701754386, Blast_Score=177, Evalue=8e-45,
Organism=Escherichia coli, GI1786312, Length=264, Percent_Identity=35.6060606060606, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25154106, Length=276, Percent_Identity=32.9710144927536, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=33.4801762114537, Blast_Score=141, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6325326, Length=287, Percent_Identity=32.7526132404181, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=290, Percent_Identity=28.6206896551724, Blast_Score=145, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24645443, Length=284, Percent_Identity=32.7464788732394, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21355941, Length=221, Percent_Identity=32.1266968325792, Blast_Score=130, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32891; Mature: 32891

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFV
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECEEEEECCCCCE
YNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFP
ECEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
DFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPFGHTEGLFTKEFYGNSYRALK
HHHCCCCCCEEEEEECCCHHHHHCCCCCCEEEEECCCCCCCCCCCCEEHHHCCCCCEEEC
EDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVYQAHIPTSPAGYWLFGFASKKYH
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCEEEEEECCCCCC
PVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVEDILEEEEGKK
CCCCCCCCCHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFV
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECEEEEECCCCCE
YNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFP
ECEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
DFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPFGHTEGLFTKEFYGNSYRALK
HHHCCCCCCEEEEEECCCHHHHHCCCCCCEEEEECCCCCCCCCCCCEEHHHCCCCCEEEC
EDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVYQAHIPTSPAGYWLFGFASKKYH
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCEEEEEECCCCCC
PVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVEDILEEEEGKK
CCCCCCCCCHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]