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The map label for this gene is radA [H]

Identifier: 169832478

GI number: 169832478

Start: 100369

End: 101631

Strand: Direct

Name: radA [H]

Synonym: SPH_0124

Alternate gene names: 169832478

Gene position: 100369-101631 (Clockwise)

Preceding gene: 169834131

Following gene: 169833040

Centisome position: 4.47

GC content: 47.98

Gene sequence:

>1263_bases
GTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAACCAAGCCCATGAAACTAGC
TGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTGCTTGGAGGCGGAGTGGTAC
CAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACAAGTCTCAACCCAGTTGTCC
CAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTGCAGAGCGCTTAGGTGATAT
TGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAGCGTATCCAGCCAGACTTTC
TCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGTTTCTCAGGTGCGTGAAGTG
ACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATGTGACCAAAGAAGGAACCTT
GGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGTCACCACACCTTTCGTATTT
TGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTCGGGCGGCTTGGTTGAGGTA
CTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCGTTGTAACCATGGAAGGGAC
GCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAGCGTACTACGACAGGACTTG
ATTTTAATCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCAAAATCAGGATGCCTATCTC
AAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTATTGCTTCGAGCTACAAAGA
CAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGGCGCGTGAATCGTATTGAGC
AACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTCAGAATTCCTTGACAGGAATCACTCTGCCT
AAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCATAA

Upstream 100 bases:

>100_bases
CATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACT
GTGGGTCTTGGTCTTCTTTT

Downstream 100 bases:

>100_bases
TCCGTGACAAATTCTCTTAAAAATGATAAGATAGGAGAAATATTTGACTATCAAATTTTCAAGGAGGGAATCGTGTCGTA
TTTTGAACAGTTTATGCAAG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLS
QVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREV
TAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEV
LNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYL
KSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLP
KEIQVIGVTTIQEVLKKVFA

Sequences:

>Translated_420_residues
MEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLS
QVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREV
TAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEV
LNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYL
KSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLP
KEIQVIGVTTIQEVLKKVFA
>Mature_420_residues
MEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLS
QVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREV
TAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEV
LNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYL
KSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLP
KEIQVIGVTTIQEVLKKVFA

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=396, Percent_Identity=45.4545454545455, Blast_Score=347, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 45806; Mature: 45806

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLI
CCCCCHHHHCCCEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEE
GGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQ
ECCCCCCCCEEEEEEHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCHH
SVRAEVERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGH
HHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
VTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEV
ECCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
LNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASL
CCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCHHHCCCCCCEECCCCCCHHHH
IMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGEL
HHHHHHHHCCEEEECCCHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHEEECC
GLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKKVFA
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLI
CCCCCHHHHCCCEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEE
GGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQ
ECCCCCCCCEEEEEEHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCHH
SVRAEVERIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGH
HHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
VTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEV
ECCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
LNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASL
CCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCHHHCCCCCCEECCCCCCHHHH
IMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGEL
HHHHHHHHCCEEEECCCHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHEEECC
GLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKKVFA
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]