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The map label for this gene is mraW [H]

Identifier: 169832477

GI number: 169832477

Start: 392011

End: 392961

Strand: Direct

Name: mraW [H]

Synonym: SPH_0444

Alternate gene names: 169832477

Gene position: 392011-392961 (Clockwise)

Preceding gene: 169833855

Following gene: 169833357

Centisome position: 17.46

GC content: 43.11

Gene sequence:

>951_bases
ATGACAAAAGAATTTCATCATGTAACGGTCTTACTCCACGAAACGATTGATATGCTCGATGTAAAACCTGACGGTATCTA
CGTTGATGCGACTTTGGGTGGAGCAGGACATAGCGAATATTTATTAAGTAAATTAAGTGAAAAAGGTCATCTCTATGCCT
TTGACCAGGACCAGAATGCCATTGACAATGCGCAAAAACGTTTGGCACCCTATATCGAAAAAGGGATGGTGACCTTTATA
AAGGATAACTTCCGTCATTTACAGGCACGTTTGCGCGAAGCTGGTGTTCAGGAAATTGATGGAATTTGTTATGACTTAGG
AGTGTCTAGTCCTCAATTGGATCAGCGTGAGCGTGGTTTTTCTTATAAAAAGGATGCGCCACTAGACATGCGCATGAATC
AGGATGCTAGTCTGACAGCCTATGAAGTGGTGAACCACTATGACTATCATGACTTGGTTCGTATTTTCTTCAAATATGGC
GAGGACAAATTCTCTAAACAGATTGCGCGTAAGATTGAACAAGAACGGGAAGTGAAGCCAATTGAGACAACGACTGAGTT
GGCTGAGATTATCAAGTCTGCTAAACCTGCTAAGGAACTCAAGAAAAAGGGCCATCCTGCCAAGCAGATTTTCCAGTCTA
TTCGGATTGAAGTCAATGATGAACTGGGAGCCGCAGATGAATCTATCCAGCAGGCTATGGATATGTTGGCTCTGGATGGT
AGAATTTCAGTGATTACCTTTCATTCCTTGGAAGACCGCTTGACCAAGCAATTATTCAAGGAAGCTTCAACAGTTGAAGT
TCCAAAAGGCTTGCCTTTCATCCCAGATGATCTCAAGCCCAAGATGGAATTGGTGTCCCGTAAGCCAATCTTGCCAAGTG
CAGAAGAATTAGAAGCCAATAACCGCTCGCACTCAGCCAAGTTGCGCGTGGCCAGAAAAATTCACAAGTAA

Upstream 100 bases:

>100_bases
AAGTATTTTTGACATATTTTTTGAAGAAATTCTTACTTGTTTGTGGCCTATTTGTCTGACTAGTGCAAGCTAGTCAGATT
TGTGGTAAAATAGATAAGAT

Downstream 100 bases:

>100_bases
GAGGGAAAAAGATGGCAGAAAGAATGGAAAAAACAGGTCAAATACTACAGATGCAACTTAAACGGTTTTCGCGTGTGGAA
AAAGCTTTTTACTTTTCCAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFI
KDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
EDKFSKQIARKIEQEREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQSIRIEVNDELGAADESIQQAMDMLALDG
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK

Sequences:

>Translated_316_residues
MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFI
KDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
EDKFSKQIARKIEQEREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQSIRIEVNDELGAADESIQQAMDMLALDG
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK
>Mature_315_residues
TKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIK
DNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYGE
DKFSKQIARKIEQEREVKPIETTTELAEIIKSAKPAKELKKKGHPAKQIFQSIRIEVNDELGAADESIQQAMDMLALDGR
ISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=35.7771260997067, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=40.1041666666667, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=40.1253918495298, Blast_Score=236, Evalue=1e-63,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=36.2537764350453, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=36.2537764350453, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 36044; Mature: 35912

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHH
IDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCCCCCCHHHCCC
SYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYGEDKFSKQIARKIEQEREVKP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
IETTTELAEIIKSAKPAKELKKKGHPAKQIFQSIRIEVNDELGAADESIQQAMDMLALDG
CHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHCCC
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEAN
CEEEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCC
NRSHSAKLRVARKIHK
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSKLSEKGHLYAFDQDQNA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHH
IDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGICYDLGVSSPQLDQRERGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCCCCCCHHHCCC
SYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYGEDKFSKQIARKIEQEREVKP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
IETTTELAEIIKSAKPAKELKKKGHPAKQIFQSIRIEVNDELGAADESIQQAMDMLALDG
CHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHCCC
RISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPDDLKPKMELVSRKPILPSAEELEAN
CEEEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCC
NRSHSAKLRVARKIHK
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA