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The map label for this gene is queA [H]

Identifier: 169832445

GI number: 169832445

Start: 1430102

End: 1431130

Strand: Direct

Name: queA [H]

Synonym: SPH_1547

Alternate gene names: 169832445

Gene position: 1430102-1431130 (Clockwise)

Preceding gene: 169834512

Following gene: 169833767

Centisome position: 63.68

GC content: 47.62

Gene sequence:

>1029_bases
ATGAATACAGCTGATTTTGATTTCCACTTGCCAGAGGAATTGATTGCCCAAACGCCCCTTGAAAAACGTGATGCCTCCAA
ACTCCTCATCGTCAACCGTGAGACAGGAGAAATGCAAGATAAACACTTCCACTCTATTATTGATATGCTGGAACCTGGTG
ATGCCCTTGTCATGAACGACACCCGAGTTCTCCCTGCCCGCCTCTATGGTCAAAAAGTGGAGACAGGAGGTCATGTGGAA
CTTCTCCTCCTTAAGAACACTAGTGGAGACGAGTGGGAAGTTCTGGCTAAACCTGCCAAACGCCTCAAGGTCGGTACTCG
TATCAGCTTTGGTGATGGCCGCCTCAGCGCTGTCGTTACAGAAGAATTGACCCACGGGGGACGCATTGTCCGCTTTGAAT
ACCAAGGAATTTTCCTAGAAGTCTTGGAAAGTCTGGGAGAAATGCCTCTGCCACCTTATATCCACGAAAAATTAGATGAC
CGTGAACGTTATCAAACCGTCTACGCCAAGGAAAGCGGCTCTGCTGCAGCACCGACTGCTGGTCTTCACTTCACCAAAGA
ACTGCTGGCAGAAATCCAAGCTAAGGGTGTTCATCTAGTCTATCTGACTCTCCATGTCGGACTCGGAACCTTTAGACCTG
TTTCTGTGGATAATCTGGACGAACACGAAATGCACTCAGAGTTCTATCAACTTTCTGAGGAAGCTGCTGCCACCCTTCGC
TCTGTCAAAAAAAATGGTGGTCGTGTCATCGCTGTCGGAACCACTTCTATCCGCACCTTGGAAACTATTGGTTCCAAGTT
TGATGGGCAAATCCAAGCAGATTCTGGTTGGACCAATATCTTTATCAAACCTGGGTATGAGTGGAAGGTCGTGGATGCCT
TCTCAACCAACTTCCATCTGCCAAAATCAACTCTGGTCATGTTGGTTTCTGCCTTTGCAGGCCGTGAATTAGTCTTAGAT
GCCTACCACCATGCCATCCAAGAACACTACCGCTTCTTCAGTTTTGGTGACGCCATGTTTATTTATTGA

Upstream 100 bases:

>100_bases
TTTGTCAAGTGAAAACGATTTAATTTCTAAAAAAAGAGCGTCCAAACTTGAAACGTGTTATAATGGATAGGATTAGAACT
TAGAAAGAATGAACAGATAT

Downstream 100 bases:

>100_bases
GAAAGAATTTCTCTAAATCTTCTAATACCAATAAATCGCTAAGATATTATTTCAAAGAACATCTACAATTGAAACTCTAG
CTAGCTGTAGAAGAGGCCTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVE
LLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
SVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLD
AYHHAIQEHYRFFSFGDAMFIY

Sequences:

>Translated_342_residues
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVE
LLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
SVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLD
AYHHAIQEHYRFFSFGDAMFIY
>Mature_342_residues
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVE
LLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
SVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLD
AYHHAIQEHYRFFSFGDAMFIY

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=46.685878962536, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38364; Mature: 38364

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVT
CEECHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHCEECCEEEECCCCEEEEEE
EELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDDRERYQTVYAKESGSAAAPTA
HHHHCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHCCHHHHHHEEEEECCCCCCCCCC
GLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
CHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCC
PKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFSFGDAMFIY
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFHSIIDMLEPGDALVMND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVT
CEECHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHCEECCEEEECCCCEEEEEE
EELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDDRERYQTVYAKESGSAAAPTA
HHHHCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHCCHHHHHHEEEEECCCCCCCCCC
GLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLR
CHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHL
HHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCC
PKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFSFGDAMFIY
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA