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The map label for this gene is prfB [H]

Identifier: 169832431

GI number: 169832431

Start: 792577

End: 793566

Strand: Direct

Name: prfB [H]

Synonym: SPH_0856

Alternate gene names: 169832431

Gene position: 792577-793566 (Clockwise)

Preceding gene: 169833130

Following gene: 169833016

Centisome position: 35.29

GC content: 42.53

Gene sequence:

>990_bases
TTGGAAAACAAGATGACAGAACCTGATTTTTGGAACGATAATATTGCGGCCCAAAAAACGTCGCAAGAATTAAATGAATT
AAAAAACACTTACAATACCTTCCATAAGATGGAAGAGTTGCAGGATGAAGTCGAAATTTTATTGGATTTTTTGGCTGAAG
ACGAGTCAGTGCATGATGAACTGGTAGCGCAGTTAGCCGAACTTGATAAGATAATGACCAGCTACGAGATGACTCTACTC
TTGTCAGAACCTTATGACCACAACAATGCCATCTTGGAAATCCATCCAGGTTCTGGTGGTACTGAGGCGCAGGACTGGGG
TGATATGTTGCTTCGTATGTATACTCGTTATGGTAATGCTAAAGGCTTTAAAGTGGAAGTGTTGGATTACCAAGCAGGTG
ATGAGGCTGGTATTAAGTCGGTAACTTTATCATTTGAAGGGCCTAATGCCTATGGTCTCCTCAAGTCAGAAATGGGTGTT
CACCGCTTAGTGCGAATCTCACCATTTGACTCTGCCAAACGTCGCCATACCTCTTTCACATCTGTAGAAGTGATGCCAGA
ATTGGATGATACTATTGAAGTGGAAATCCGTGAAGATGATATCAAGATGGATACCTTCCGTTCAGGTGGTGCCGGTGGAC
AAAACGTCAATAAGGTTTCAACAGGTGTACGTTTAACCCACATTCCAACTGGAATTGTTGTCCAATCAACAGTGGATCGT
ACCCAGTATGGAAATAGAGATCGTGCCATGAAGATGTTGCAGGCTAAGCTCTATCAAATGGAGCAAGAGAAGAAGGCTGC
GGAGGTAGATTCTCTCAAAGGTGAGAAAAAGGAGATTACTTGGGGAAGTCAAATCCGTTCTTATGTCTTCACGCCTTATA
CTATGGTAAAAGATCACCGAACTAGCTTTGAGGTTGCTCAGGTAGATAAGGTTATGGATGGGGACCTAGATGGTTTTATC
GATGCTTATCTCAAGTGGCGAATTAGCTAA

Upstream 100 bases:

>100_bases
ATTTCAGTAATTCGTCAGAAAATTGACGCAAATCGTGAAAAATTAGCTTCTTTCAGGGGGTCTCTTTGACCTCGAAGGGC
TAGAGGAAGAGATTGCCATC

Downstream 100 bases:

>100_bases
GATAGAAAGGAACTCACATGTCAATTATTGAAATGAGAGATGTCGTTAAAAAATACGACAACGGAACGACTGCTCTACGC
GGTGTTTCGGTCAGCGTTCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MENKMTEPDFWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAELDKIMTSYEMTLL
LSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGV
HRLVRISPFDSAKRRHTSFTSVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDR
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFI
DAYLKWRIS

Sequences:

>Translated_329_residues
MENKMTEPDFWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAELDKIMTSYEMTLL
LSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGV
HRLVRISPFDSAKRRHTSFTSVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDR
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFI
DAYLKWRIS
>Mature_329_residues
MENKMTEPDFWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAELDKIMTSYEMTLL
LSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGV
HRLVRISPFDSAKRRHTSFTSVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDR
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFI
DAYLKWRIS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=316, Percent_Identity=32.5949367088608, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=33.9869281045752, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=34.1346153846154, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=46.9135802469136, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1787462, Length=309, Percent_Identity=34.6278317152104, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=298, Percent_Identity=30.5369127516779, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=312, Percent_Identity=32.6923076923077, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19921226, Length=265, Percent_Identity=32.4528301886792, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37450; Mature: 37450

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKMTEPDFWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVAQLAELDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
KGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFT
CCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHEEEECCCCHHHHHCCCCC
SVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDR
EEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHCCEEEEECCCCEEEECHHHH
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCC
TSFEVAQVDKVMDGDLDGFIDAYLKWRIS
CCHHHHHHHHHHCCCHHHHHHHHHEEECC
>Mature Secondary Structure
MENKMTEPDFWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVAQLAELDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
KGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFT
CCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCHHHEEEECCCCHHHHHCCCCC
SVEVMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDR
EEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHCCEEEEECCCCEEEECHHHH
TQYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHCCC
TSFEVAQVDKVMDGDLDGFIDAYLKWRIS
CCHHHHHHHHHHCCCHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA