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The map label for this gene is 169825263

Identifier: 169825263

GI number: 169825263

Start: 1738320

End: 1739624

Strand: Direct

Name: 169825263

Synonym: FMG_1566

Alternate gene names: NA

Gene position: 1738320-1739624 (Clockwise)

Preceding gene: 169825262

Following gene: 169825264

Centisome position: 96.7

GC content: 36.17

Gene sequence:

>1305_bases
ATGGATTACGAAAAACAGCTTGAAGCTGTAATTAACTACATTAAAAGCGGAGAAAAACTAAAAGAAGATTTCACTATAGG
AATGGAAATGGAACATTTCATCGTAGACAAGGATACTTTGAAGACTGTGTCGTATTTCGGTGATAATGGAGTAGGTGCAA
CTCTCAGAGAACTTCACAATCATGGATGTGCAGCTTACAAAGAGGGCGATTACATCCTTGGACTTGAAAATGATGATTTG
GCAGTGTCGACAGAACCTGGAAGTCAGTTCGAGGTTGCTTTGTCATCGAAGTGGAACATCGACGATTTGAAGGATATTTA
CATCAAAAATATGAGAAAACTTGTCGAAATTTTCGATAAGAAAAACCAAGCTATGGTGACTTTGGGATATCATCCTGTGA
CTAAAATCGACGAGATTAAAATCTTGCCTAAGAAAAGATACGATTTTATGTACAAATATTTCAACGAAAGAGGAGACATG
GCTCACAACATGATGAAGGGCACTTGTTCTTTGCAATGTAGCATTGACTATTCATCAGAAAAAGATTTCGTTAGAAAATA
CAAGATTGCCAACTGTTTGTCTCCAGTTTTCTACACATTGTTCGACAACGCATATATTTTCGAAGGAGAGCCTTCAAAAA
GTCGTAACATGAGAGAAATCATTTGGGAAAACACAGACACTGACAGATCTGGTATGTATAAGTTTAGCTTCGATGATGAT
TTGAGCTACAAAAAATACGCCGAACATATTTTGAACACGCCGTTGATTTTCTCAATTGATGAAAACCACGAGCCTTATTA
TGTCGGAAAGACAACTTTCAAGGAAGTATTTGAAGATGTGAAGGACACTGGTTTGATTTTCCACGCATTATCAATAGTAT
TTCCAGATGTAAGGGCGAAACGATACATTGAAATCCGAATGATGGACGAAATCAAATATCCACTTAATTTCTCAGCAGTA
GCCCTAATCAAAGGATTGTTCTACGACGAGGTTAATTTGGACAAATTATCAGAGCTATTCAAAAACATGACATATGAAAA
TTGCATGAAAGCAAAACTAGATGCACGTGAAAAAGGACTGGATGCTACATTCATGAATGTAAACATGTTAGAGTTTTGCA
AAATGCTTGTGGATATGGCAAAAAATGGACTTCCTGCAAATGAAATCGGATATTTAATTCCACTAGAAGAAATGTTGGAA
AAAGGAATGAATCCAAGAGATCAGTTCGAGAAAATTTACAAGGAAAAAGGGCTTCGTGAAGCTGTAGTTGCAAATGAATT
AAAATTGGAGGATTTAGATGTATAA

Upstream 100 bases:

>100_bases
ATGTGTCTGGCGGCAAAATCGGTGCGCAAATCAAAATCGCTCCCCAAGATTTGATAAAAATTTGTGATATTACTGTGGCG
GACCTTAAAAAGGAGTAAAA

Downstream 100 bases:

>100_bases
TGACGAATATTCATTAGAATTATATAAAATAGTACGAGAAGACGAGGACATGTATTACAGGGATTTCGTGGATTTTATCG
CCAAAACAAAAGTGTCAAAG

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDYEKQLEAVINYIKSGEKLKEDFTIGMEMEHFIVDKDTLKTVSYFGDNGVGATLRELHNHGCAAYKEGDYILGLENDDL
AVSTEPGSQFEVALSSKWNIDDLKDIYIKNMRKLVEIFDKKNQAMVTLGYHPVTKIDEIKILPKKRYDFMYKYFNERGDM
AHNMMKGTCSLQCSIDYSSEKDFVRKYKIANCLSPVFYTLFDNAYIFEGEPSKSRNMREIIWENTDTDRSGMYKFSFDDD
LSYKKYAEHILNTPLIFSIDENHEPYYVGKTTFKEVFEDVKDTGLIFHALSIVFPDVRAKRYIEIRMMDEIKYPLNFSAV
ALIKGLFYDEVNLDKLSELFKNMTYENCMKAKLDAREKGLDATFMNVNMLEFCKMLVDMAKNGLPANEIGYLIPLEEMLE
KGMNPRDQFEKIYKEKGLREAVVANELKLEDLDV

Sequences:

>Translated_434_residues
MDYEKQLEAVINYIKSGEKLKEDFTIGMEMEHFIVDKDTLKTVSYFGDNGVGATLRELHNHGCAAYKEGDYILGLENDDL
AVSTEPGSQFEVALSSKWNIDDLKDIYIKNMRKLVEIFDKKNQAMVTLGYHPVTKIDEIKILPKKRYDFMYKYFNERGDM
AHNMMKGTCSLQCSIDYSSEKDFVRKYKIANCLSPVFYTLFDNAYIFEGEPSKSRNMREIIWENTDTDRSGMYKFSFDDD
LSYKKYAEHILNTPLIFSIDENHEPYYVGKTTFKEVFEDVKDTGLIFHALSIVFPDVRAKRYIEIRMMDEIKYPLNFSAV
ALIKGLFYDEVNLDKLSELFKNMTYENCMKAKLDAREKGLDATFMNVNMLEFCKMLVDMAKNGLPANEIGYLIPLEEMLE
KGMNPRDQFEKIYKEKGLREAVVANELKLEDLDV
>Mature_434_residues
MDYEKQLEAVINYIKSGEKLKEDFTIGMEMEHFIVDKDTLKTVSYFGDNGVGATLRELHNHGCAAYKEGDYILGLENDDL
AVSTEPGSQFEVALSSKWNIDDLKDIYIKNMRKLVEIFDKKNQAMVTLGYHPVTKIDEIKILPKKRYDFMYKYFNERGDM
AHNMMKGTCSLQCSIDYSSEKDFVRKYKIANCLSPVFYTLFDNAYIFEGEPSKSRNMREIIWENTDTDRSGMYKFSFDDD
LSYKKYAEHILNTPLIFSIDENHEPYYVGKTTFKEVFEDVKDTGLIFHALSIVFPDVRAKRYIEIRMMDEIKYPLNFSAV
ALIKGLFYDEVNLDKLSELFKNMTYENCMKAKLDAREKGLDATFMNVNMLEFCKMLVDMAKNGLPANEIGYLIPLEEMLE
KGMNPRDQFEKIYKEKGLREAVVANELKLEDLDV

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 50521; Mature: 50521

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYEKQLEAVINYIKSGEKLKEDFTIGMEMEHFIVDKDTLKTVSYFGDNGVGATLRELHN
CCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHEEECHHHHHHHHHCCCCCCHHHHHHHHC
HGCAAYKEGDYILGLENDDLAVSTEPGSQFEVALSSKWNIDDLKDIYIKNMRKLVEIFDK
CCCCEEECCCEEEEECCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
KNQAMVTLGYHPVTKIDEIKILPKKRYDFMYKYFNERGDMAHNMMKGTCSLQCSIDYSSE
CCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCC
KDFVRKYKIANCLSPVFYTLFDNAYIFEGEPSKSRNMREIIWENTDTDRSGMYKFSFDDD
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCC
LSYKKYAEHILNTPLIFSIDENHEPYYVGKTTFKEVFEDVKDTGLIFHALSIVFPDVRAK
CCHHHHHHHHCCCCEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RYIEIRMMDEIKYPLNFSAVALIKGLFYDEVNLDKLSELFKNMTYENCMKAKLDAREKGL
EEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
DATFMNVNMLEFCKMLVDMAKNGLPANEIGYLIPLEEMLEKGMNPRDQFEKIYKEKGLRE
CEEEECCCHHHHHHHHHHHHHCCCCHHHCCEEECHHHHHHCCCCHHHHHHHHHHHCCCHH
AVVANELKLEDLDV
HHHHCCCCCCCCCC
>Mature Secondary Structure
MDYEKQLEAVINYIKSGEKLKEDFTIGMEMEHFIVDKDTLKTVSYFGDNGVGATLRELHN
CCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHEEECHHHHHHHHHCCCCCCHHHHHHHHC
HGCAAYKEGDYILGLENDDLAVSTEPGSQFEVALSSKWNIDDLKDIYIKNMRKLVEIFDK
CCCCEEECCCEEEEECCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
KNQAMVTLGYHPVTKIDEIKILPKKRYDFMYKYFNERGDMAHNMMKGTCSLQCSIDYSSE
CCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCC
KDFVRKYKIANCLSPVFYTLFDNAYIFEGEPSKSRNMREIIWENTDTDRSGMYKFSFDDD
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCC
LSYKKYAEHILNTPLIFSIDENHEPYYVGKTTFKEVFEDVKDTGLIFHALSIVFPDVRAK
CCHHHHHHHHCCCCEEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RYIEIRMMDEIKYPLNFSAVALIKGLFYDEVNLDKLSELFKNMTYENCMKAKLDAREKGL
EEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
DATFMNVNMLEFCKMLVDMAKNGLPANEIGYLIPLEEMLEKGMNPRDQFEKIYKEKGLRE
CEEEECCCHHHHHHHHHHHHHCCCCHHHCCEEECHHHHHHCCCCHHHHHHHHHHHCCCHH
AVVANELKLEDLDV
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA