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The map label for this gene is glyS [H]

Identifier: 169824435

GI number: 169824435

Start: 800334

End: 802022

Strand: Direct

Name: glyS [H]

Synonym: FMG_0738

Alternate gene names: 169824435

Gene position: 800334-802022 (Clockwise)

Preceding gene: 169824434

Following gene: 169824436

Centisome position: 44.52

GC content: 23.98

Gene sequence:

>1689_bases
ATGCACAACTTATTAATTGATATAGTGACTAAGAATATACCTAATGACTTTATTGGATTTATAAATAGATCACTTTACGA
AAAAATAAGAAAAGAATTTAAAAAACTTCAATTACTATATTCAAATATAGATTTGGATACAATAAAAGATCATTTTTTTA
TTTATGTTACTAGCATAGAAAATACCAAATACACGCTAGAAGATTACGAAACCATAATAATTAATGCAATTGATTCTATA
AATTTTAAATATAAATATAATCACGAGAGTTTATCATTTAAAGAGTCTATTTTGCAAATAAAAATTTTTAATGAAGAACA
GCATAAAACAATAAATTTTAATGATGTTGATGAAAATAAGAATAACGAATTAGTTAATTCTTCTACTTTTAACGATTTTT
CCTCTGCGTTATTGAAAAATGAAATTATATTGAAAAAAGACCAGAGACTAGAATCTTTTGAAAAAAGTGCTAATAAACTT
GCAAGAGCTAACGGATGCTCAATAATTAATAATAAGAGCATAATGAATGAATATGCTAATAAGTTTGATTATCCTGTAAG
TGTAGAAGGTTCATTCGACGAAAAATATCTATGCATTGATAAATCAATACTATATACAGTTTTAATGGAAAATTTTAAGG
TATTACCAATGGAATTTGAAAATGGAAATAGTTCTAACTTTTTTGTCTATGCTAAAAATAAAAATTCCGAAGACTCTCGA
GTTTTGTGTGCTGAAATTAATAATAAATTGGAAGAAATAAATAATTTGATAACTAAAAAGTCAGATAAAAAACTTGAAGA
TTTTGTAGAAGATTTAAAAAACGTTACTTATATTAATAATTATGGTAACTTTTATGATAAAACACAAAGACTAATAAAAA
TATCTCCTGAATTATGTAAGATGCTTTCGGTTAGCGAAGAAACAGTTAATTCTGTAAACAGAATTTCATTTCTGTGTAAA
GCAGATAAAGCAACAGAAATTGTTAAAAAGTGTCCTTACTTACATGGATTAATTGGTAGATTGTATGCTAAGTCTGATGG
AGAAGAAAATATTGTTTATGATGGTGTTTATGAGCATATTAAACCAAGATTTATATCTGAAAGTATTCCAAATTCAACAG
CGGCTAGAATTGTAGCAATTTCTGATAAAATGGAATATGTTATGTCTTACATAATATCAAGCAGTGACGAAAATTTTAAA
TATAATTACAAGCTAAGAAAAACTATTACATCGGTGATATCTATTATTCTCAAATCAGAATTGGATTTTAATTTAAGTGA
TTTTATTACTAAAATTTTATATGTATTTTTACAAGATAAAGGTATAGTAGATTATGAATATTTTGAGAAAAGTATAAAAG
AAATTATTCTTGATGTTTACAGAGACGAATGCATAAAATCAGGGTATGATTTTGATGAAATTGATGTGATTAATTATGCT
AATTTAAATATTTTTAAATTAAATCGTTTATTGACAAACTTACATGAAATAAATTCAAAATATAATGGTAAATTAATGTT
ACAGATTCAAAAAATGCTAGAGGTAAATTGTAATGAGTGTAAGTATACACCGATGGAATTAGATAGCAATAATTTGTGTG
ATATGTTTGAAAGATTATCAAAATGCTCTGATGAAGATTTGGCTTTTTATAAATTAATTTTTAAAGGTGTATTTAATAAA
TTAAATTAA

Upstream 100 bases:

>100_bases
GAATGAAATTTTAAAGATTTTGCTAAAATACACGATGAACAATTTTGGAATAGAATATTTAGGGTCATTAGGTTACCTTG
AATATTTGTAAGGAGAAATA

Downstream 100 bases:

>100_bases
GGAGAATGTTATGAATAAAACTATATTAGTTATTTCTGATTCTACAGGAGAGACAGCAAATCAAGTTTTGGATGCTGTGA
AAGTTCATTTTGATATTACA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MHNLLIDIVTKNIPNDFIGFINRSLYEKIRKEFKKLQLLYSNIDLDTIKDHFFIYVTSIENTKYTLEDYETIIINAIDSI
NFKYKYNHESLSFKESILQIKIFNEEQHKTINFNDVDENKNNELVNSSTFNDFSSALLKNEIILKKDQRLESFEKSANKL
ARANGCSIINNKSIMNEYANKFDYPVSVEGSFDEKYLCIDKSILYTVLMENFKVLPMEFENGNSSNFFVYAKNKNSEDSR
VLCAEINNKLEEINNLITKKSDKKLEDFVEDLKNVTYINNYGNFYDKTQRLIKISPELCKMLSVSEETVNSVNRISFLCK
ADKATEIVKKCPYLHGLIGRLYAKSDGEENIVYDGVYEHIKPRFISESIPNSTAARIVAISDKMEYVMSYIISSSDENFK
YNYKLRKTITSVISIILKSELDFNLSDFITKILYVFLQDKGIVDYEYFEKSIKEIILDVYRDECIKSGYDFDEIDVINYA
NLNIFKLNRLLTNLHEINSKYNGKLMLQIQKMLEVNCNECKYTPMELDSNNLCDMFERLSKCSDEDLAFYKLIFKGVFNK
LN

Sequences:

>Translated_562_residues
MHNLLIDIVTKNIPNDFIGFINRSLYEKIRKEFKKLQLLYSNIDLDTIKDHFFIYVTSIENTKYTLEDYETIIINAIDSI
NFKYKYNHESLSFKESILQIKIFNEEQHKTINFNDVDENKNNELVNSSTFNDFSSALLKNEIILKKDQRLESFEKSANKL
ARANGCSIINNKSIMNEYANKFDYPVSVEGSFDEKYLCIDKSILYTVLMENFKVLPMEFENGNSSNFFVYAKNKNSEDSR
VLCAEINNKLEEINNLITKKSDKKLEDFVEDLKNVTYINNYGNFYDKTQRLIKISPELCKMLSVSEETVNSVNRISFLCK
ADKATEIVKKCPYLHGLIGRLYAKSDGEENIVYDGVYEHIKPRFISESIPNSTAARIVAISDKMEYVMSYIISSSDENFK
YNYKLRKTITSVISIILKSELDFNLSDFITKILYVFLQDKGIVDYEYFEKSIKEIILDVYRDECIKSGYDFDEIDVINYA
NLNIFKLNRLLTNLHEINSKYNGKLMLQIQKMLEVNCNECKYTPMELDSNNLCDMFERLSKCSDEDLAFYKLIFKGVFNK
LN
>Mature_562_residues
MHNLLIDIVTKNIPNDFIGFINRSLYEKIRKEFKKLQLLYSNIDLDTIKDHFFIYVTSIENTKYTLEDYETIIINAIDSI
NFKYKYNHESLSFKESILQIKIFNEEQHKTINFNDVDENKNNELVNSSTFNDFSSALLKNEIILKKDQRLESFEKSANKL
ARANGCSIINNKSIMNEYANKFDYPVSVEGSFDEKYLCIDKSILYTVLMENFKVLPMEFENGNSSNFFVYAKNKNSEDSR
VLCAEINNKLEEINNLITKKSDKKLEDFVEDLKNVTYINNYGNFYDKTQRLIKISPELCKMLSVSEETVNSVNRISFLCK
ADKATEIVKKCPYLHGLIGRLYAKSDGEENIVYDGVYEHIKPRFISESIPNSTAARIVAISDKMEYVMSYIISSSDENFK
YNYKLRKTITSVISIILKSELDFNLSDFITKILYVFLQDKGIVDYEYFEKSIKEIILDVYRDECIKSGYDFDEIDVINYA
NLNIFKLNRLLTNLHEINSKYNGKLMLQIQKMLEVNCNECKYTPMELDSNNLCDMFERLSKCSDEDLAFYKLIFKGVFNK
LN

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=347, Percent_Identity=23.0547550432277, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 65717; Mature: 65717

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLLIDIVTKNIPNDFIGFINRSLYEKIRKEFKKLQLLYSNIDLDTIKDHFFIYVTSIE
CCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEEEEC
NTKYTLEDYETIIINAIDSINFKYKYNHESLSFKESILQIKIFNEEQHKTINFNDVDENK
CCEEEHHHHHEEEEEEHHCCCEEEEECCCCHHHHHCEEEEEEECCCCCCEECCCCCCCCC
NNELVNSSTFNDFSSALLKNEIILKKDQRLESFEKSANKLARANGCSIINNKSIMNEYAN
CCCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHH
KFDYPVSVEGSFDEKYLCIDKSILYTVLMENFKVLPMEFENGNSSNFFVYAKNKNSEDSR
HCCCCEEECCCCCCCEEEEHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCCCCCCCE
VLCAEINNKLEEINNLITKKSDKKLEDFVEDLKNVTYINNYGNFYDKTQRLIKISPELCK
EEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCHHHHH
MLSVSEETVNSVNRISFLCKADKATEIVKKCPYLHGLIGRLYAKSDGEENIVYDGVYEHI
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEECCHHHHC
KPRFISESIPNSTAARIVAISDKMEYVMSYIISSSDENFKYNYKLRKTITSVISIILKSE
CCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHC
LDFNLSDFITKILYVFLQDKGIVDYEYFEKSIKEIILDVYRDECIKSGYDFDEIDVINYA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NLNIFKLNRLLTNLHEINSKYNGKLMLQIQKMLEVNCNECKYTPMELDSNNLCDMFERLS
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
KCSDEDLAFYKLIFKGVFNKLN
CCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHNLLIDIVTKNIPNDFIGFINRSLYEKIRKEFKKLQLLYSNIDLDTIKDHFFIYVTSIE
CCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEEEEC
NTKYTLEDYETIIINAIDSINFKYKYNHESLSFKESILQIKIFNEEQHKTINFNDVDENK
CCEEEHHHHHEEEEEEHHCCCEEEEECCCCHHHHHCEEEEEEECCCCCCEECCCCCCCCC
NNELVNSSTFNDFSSALLKNEIILKKDQRLESFEKSANKLARANGCSIINNKSIMNEYAN
CCCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHH
KFDYPVSVEGSFDEKYLCIDKSILYTVLMENFKVLPMEFENGNSSNFFVYAKNKNSEDSR
HCCCCEEECCCCCCCEEEEHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCCCCCCCE
VLCAEINNKLEEINNLITKKSDKKLEDFVEDLKNVTYINNYGNFYDKTQRLIKISPELCK
EEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCHHHHH
MLSVSEETVNSVNRISFLCKADKATEIVKKCPYLHGLIGRLYAKSDGEENIVYDGVYEHI
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEECCHHHHC
KPRFISESIPNSTAARIVAISDKMEYVMSYIISSSDENFKYNYKLRKTITSVISIILKSE
CCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHC
LDFNLSDFITKILYVFLQDKGIVDYEYFEKSIKEIILDVYRDECIKSGYDFDEIDVINYA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NLNIFKLNRLLTNLHEINSKYNGKLMLQIQKMLEVNCNECKYTPMELDSNNLCDMFERLS
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
KCSDEDLAFYKLIFKGVFNKLN
CCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA