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The map label for this gene is topA [H]

Identifier: 169824402

GI number: 169824402

Start: 768523

End: 770589

Strand: Direct

Name: topA [H]

Synonym: FMG_0705

Alternate gene names: 169824402

Gene position: 768523-770589 (Clockwise)

Preceding gene: 169824401

Following gene: 169824403

Centisome position: 42.75

GC content: 30.58

Gene sequence:

>2067_bases
ATGGCAAACAAAAAGCTAGTAGTAGTGGAATCTCCAACCAAAGCAAAAACAATTCAAAAAATGTTAGGCTCTGGATATAA
AGTTGTAGCTACTATAGGTCATTTAAGAGATTTACCGAAAAGCAAACTGGGGATAGATATAGAGAATGATTTTGAACCAC
GATATATCAATGTTCGTGGTAAAGCTAATGTTATTAATGCTTTAAAAAAAGATTATAAGAATTCTCAAAAAATATTCTTG
GCAACTGACCCTGATAGAGAAGGAGAAGCCATTTCTTGGCATTTGTGCTATTTATTAGGAATAAATCCGAAGGAAGAAAA
TAGAGTAAAATTTCAAGAGATAACAAAAGACTCCGTAAAAGAAGCAATCAAAAAGCCGGAAGCTATTGATTTAAAATTGG
TAGATGCTCAACAAGCAAGAAGAGTTTTGGATCGTATTGTAGGTTATAGCATTTCTCCATTATTATGGAAGAAAGTTAAA
TCAGGACTAAGTGCTGGTAGAGTACAGTCTGTAGCATTAAGACTAATTTGTGAAAGAGAAAAAGAAATAAGAGAGTTTGA
GCCAGAAGAATACTGGTCAATTCATGCAAACTTATCAAAAGATAAATTAGATTTTGATTCTGAACTATATTATTCAAAAA
GCGATAAATCTAATAAAATAAATATAAAAAACAAAGATGAAGCTGATAAAATTTTAAATGATTTATCTGATAAATTCGTT
GTAGACTCAATAAAGCAACAAAAAAAAGCAAGAAAACCATACGCTCCATTTACAACATCGACACTTCAACAAGAGGCATA
TAAAAAGTTATATTTTCAAACGTCAAAAACGATGAGAATTGCTCAACAGCTATATGAAGGAATTAGTTTGGGAAGCGAAG
GGTCAGTAGGTTTGATTACTTATATGAGAACTGATTCAACAAGGATTTCTCAGGTTTGTGTTAATGATTGTTTAGATATT
ATTAATGAGCTTTATGGAAAACAATATGCAACAAGAGGATATGATTATTCCAAAAAGAAAAAAGGATCACAAGATGCACA
CGAAGGAATTAGACCATCGTCTGTAAAAAGAAGACCGAATGAAATTAAAAAATATTTGACCGAAGATCAATACAAACTAT
ATAAGTTGATTTGGGAAAGAACTGTTGCTAGTCAGATGAAAGATGCAAGATATTTATCTACATCAATTGATTTTGATAAT
AATGGATACACCTTTAGATCTAATGGCAATGTTGTTATTTTTGATGGATTTACAAGAGTTTATTCATTAAACACTAAAGA
TAGTGAATTACCAGAATTAAAAGATAGAGAAGCTATATCTTCAAATGAAATCGAACCTAAACAGCACTTTACTAAACCAA
AACCTAGATATACAGAAGCAAGTTTGGTAAAAACGCTAGAAGAAGATGGTATTGGTAGACCAAGCACTTATGCGTCTATT
ATTAGCAGTATTTTGTCCAGAAATTACGTTGAACTTAAAGAAAAAAAATTCTATACAACTGAAATTGGTGAAAAAGTTTG
TGAATTTGTAGTTAAATACTTTGATTCAATTATCAATGAAAAATTTACTGCAGAGATGGAGAATGAATTAGATAATATTG
CCGAAAACGACGTTGAATGGAAAAAAGTAATTAGAGATTTTTATAATGGATTTAATGAAGATTTAGTTAATGCAAAAAAT
GCACAAGATAAATTTAAAGTTAAAGATGAACCAATTGGAAGAAAATGCCCAGAATGTGGCAATGATTTGGTAATAAAGCA
TGGAAGAAACGGAAAATTTATCGGATGCAGTGCATTTCCTGAGTGTAAGTACACAGAAACGATAGTAAAAGATACAAAGG
TAAAATGTCCAAAATGTGGACATAGAATTATTGAAAAAGTGTCTAAAAGAGGTAAGGTTTTTTATGGATGCGAAAATTAT
CCAAATTGTGACTTTGCTTTATGGGATAAACCTACTGGAGAAAAATGTCCTGAATGTGGAAGCTTATTAGTTCATAGAAA
AAATAGACGTGGTGAATCCGTACTTTGCTCTAACGAAAAATGTAATTATAAAAAGCTTGAAAAATAA

Upstream 100 bases:

>100_bases
ATAAATAAATTGGGAATAAGAAAAGATCAAATTTCTACAGAATCTATAAGTGAATTAAGATTAAAATTACAAGAAAATTA
ATAGAAAGGATAGACTTTTA

Downstream 100 bases:

>100_bases
AATTATTTATGATATACTTTCTAAGTAAAACACACAGCCGATGATACAAATACAGTTGCTTAAATGTCGTATTTGTAGGT
GAGTCGTCTGGAGGAAAAAA

Product: topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MANKKLVVVESPTKAKTIQKMLGSGYKVVATIGHLRDLPKSKLGIDIENDFEPRYINVRGKANVINALKKDYKNSQKIFL
ATDPDREGEAISWHLCYLLGINPKEENRVKFQEITKDSVKEAIKKPEAIDLKLVDAQQARRVLDRIVGYSISPLLWKKVK
SGLSAGRVQSVALRLICEREKEIREFEPEEYWSIHANLSKDKLDFDSELYYSKSDKSNKINIKNKDEADKILNDLSDKFV
VDSIKQQKKARKPYAPFTTSTLQQEAYKKLYFQTSKTMRIAQQLYEGISLGSEGSVGLITYMRTDSTRISQVCVNDCLDI
INELYGKQYATRGYDYSKKKKGSQDAHEGIRPSSVKRRPNEIKKYLTEDQYKLYKLIWERTVASQMKDARYLSTSIDFDN
NGYTFRSNGNVVIFDGFTRVYSLNTKDSELPELKDREAISSNEIEPKQHFTKPKPRYTEASLVKTLEEDGIGRPSTYASI
ISSILSRNYVELKEKKFYTTEIGEKVCEFVVKYFDSIINEKFTAEMENELDNIAENDVEWKKVIRDFYNGFNEDLVNAKN
AQDKFKVKDEPIGRKCPECGNDLVIKHGRNGKFIGCSAFPECKYTETIVKDTKVKCPKCGHRIIEKVSKRGKVFYGCENY
PNCDFALWDKPTGEKCPECGSLLVHRKNRRGESVLCSNEKCNYKKLEK

Sequences:

>Translated_688_residues
MANKKLVVVESPTKAKTIQKMLGSGYKVVATIGHLRDLPKSKLGIDIENDFEPRYINVRGKANVINALKKDYKNSQKIFL
ATDPDREGEAISWHLCYLLGINPKEENRVKFQEITKDSVKEAIKKPEAIDLKLVDAQQARRVLDRIVGYSISPLLWKKVK
SGLSAGRVQSVALRLICEREKEIREFEPEEYWSIHANLSKDKLDFDSELYYSKSDKSNKINIKNKDEADKILNDLSDKFV
VDSIKQQKKARKPYAPFTTSTLQQEAYKKLYFQTSKTMRIAQQLYEGISLGSEGSVGLITYMRTDSTRISQVCVNDCLDI
INELYGKQYATRGYDYSKKKKGSQDAHEGIRPSSVKRRPNEIKKYLTEDQYKLYKLIWERTVASQMKDARYLSTSIDFDN
NGYTFRSNGNVVIFDGFTRVYSLNTKDSELPELKDREAISSNEIEPKQHFTKPKPRYTEASLVKTLEEDGIGRPSTYASI
ISSILSRNYVELKEKKFYTTEIGEKVCEFVVKYFDSIINEKFTAEMENELDNIAENDVEWKKVIRDFYNGFNEDLVNAKN
AQDKFKVKDEPIGRKCPECGNDLVIKHGRNGKFIGCSAFPECKYTETIVKDTKVKCPKCGHRIIEKVSKRGKVFYGCENY
PNCDFALWDKPTGEKCPECGSLLVHRKNRRGESVLCSNEKCNYKKLEK
>Mature_687_residues
ANKKLVVVESPTKAKTIQKMLGSGYKVVATIGHLRDLPKSKLGIDIENDFEPRYINVRGKANVINALKKDYKNSQKIFLA
TDPDREGEAISWHLCYLLGINPKEENRVKFQEITKDSVKEAIKKPEAIDLKLVDAQQARRVLDRIVGYSISPLLWKKVKS
GLSAGRVQSVALRLICEREKEIREFEPEEYWSIHANLSKDKLDFDSELYYSKSDKSNKINIKNKDEADKILNDLSDKFVV
DSIKQQKKARKPYAPFTTSTLQQEAYKKLYFQTSKTMRIAQQLYEGISLGSEGSVGLITYMRTDSTRISQVCVNDCLDII
NELYGKQYATRGYDYSKKKKGSQDAHEGIRPSSVKRRPNEIKKYLTEDQYKLYKLIWERTVASQMKDARYLSTSIDFDNN
GYTFRSNGNVVIFDGFTRVYSLNTKDSELPELKDREAISSNEIEPKQHFTKPKPRYTEASLVKTLEEDGIGRPSTYASII
SSILSRNYVELKEKKFYTTEIGEKVCEFVVKYFDSIINEKFTAEMENELDNIAENDVEWKKVIRDFYNGFNEDLVNAKNA
QDKFKVKDEPIGRKCPECGNDLVIKHGRNGKFIGCSAFPECKYTETIVKDTKVKCPKCGHRIIEKVSKRGKVFYGCENYP
NCDFALWDKPTGEKCPECGSLLVHRKNRRGESVLCSNEKCNYKKLEK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=614, Percent_Identity=25.0814332247557, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI4507635, Length=522, Percent_Identity=23.7547892720307, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1787529, Length=753, Percent_Identity=37.9814077025232, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI48994931, Length=114, Percent_Identity=42.9824561403509, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI1788061, Length=631, Percent_Identity=21.0776545166403, Blast_Score=93, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=655, Percent_Identity=23.5114503816794, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=458, Percent_Identity=23.1441048034935, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585251, Length=677, Percent_Identity=23.9290989660266, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640096, Length=593, Percent_Identity=23.7774030354132, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79102; Mature: 78971

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKLVVVESPTKAKTIQKMLGSGYKVVATIGHLRDLPKSKLGIDIENDFEPRYINVRG
CCCCCEEEEECCHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHCCCCCCCCCCCCEEEECC
KANVINALKKDYKNSQKIFLATDPDREGEAISWHLCYLLGINPKEENRVKFQEITKDSVK
CHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
EAIKKPEAIDLKLVDAQQARRVLDRIVGYSISPLLWKKVKSGLSAGRVQSVALRLICERE
HHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KEIREFEPEEYWSIHANLSKDKLDFDSELYYSKSDKSNKINIKNKDEADKILNDLSDKFV
HHHHHCCCHHHEEEECCCCCCCCCCCCCEEECCCCCCCEECCCCCCHHHHHHHHHHHHHH
VDSIKQQKKARKPYAPFTTSTLQQEAYKKLYFQTSKTMRIAQQLYEGISLGSEGSVGLIT
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
YMRTDSTRISQVCVNDCLDIINELYGKQYATRGYDYSKKKKGSQDAHEGIRPSSVKRRPN
EEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHCCCCCHHHHCCCCHHHHCCHH
EIKKYLTEDQYKLYKLIWERTVASQMKDARYLSTSIDFDNNGYTFRSNGNVVIFDGFTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCEEEECCCEEE
YSLNTKDSELPELKDREAISSNEIEPKQHFTKPKPRYTEASLVKTLEEDGIGRPSTYASI
EECCCCCCCCCCCHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
ISSILSRNYVELKEKKFYTTEIGEKVCEFVVKYFDSIINEKFTAEMENELDNIAENDVEW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHH
KKVIRDFYNGFNEDLVNAKNAQDKFKVKDEPIGRKCPECGNDLVIKHGRNGKFIGCSAFP
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCC
ECKYTETIVKDTKVKCPKCGHRIIEKVSKRGKVFYGCENYPNCDFALWDKPTGEKCPECG
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCCCCCHHH
SLLVHRKNRRGESVLCSNEKCNYKKLEK
HHHHCCCCCCCCCEEECCCCCCCHHCCC
>Mature Secondary Structure 
ANKKLVVVESPTKAKTIQKMLGSGYKVVATIGHLRDLPKSKLGIDIENDFEPRYINVRG
CCCCEEEEECCHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHCCCCCCCCCCCCEEEECC
KANVINALKKDYKNSQKIFLATDPDREGEAISWHLCYLLGINPKEENRVKFQEITKDSVK
CHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
EAIKKPEAIDLKLVDAQQARRVLDRIVGYSISPLLWKKVKSGLSAGRVQSVALRLICERE
HHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KEIREFEPEEYWSIHANLSKDKLDFDSELYYSKSDKSNKINIKNKDEADKILNDLSDKFV
HHHHHCCCHHHEEEECCCCCCCCCCCCCEEECCCCCCCEECCCCCCHHHHHHHHHHHHHH
VDSIKQQKKARKPYAPFTTSTLQQEAYKKLYFQTSKTMRIAQQLYEGISLGSEGSVGLIT
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
YMRTDSTRISQVCVNDCLDIINELYGKQYATRGYDYSKKKKGSQDAHEGIRPSSVKRRPN
EEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHCCCCCHHHHCCCCHHHHCCHH
EIKKYLTEDQYKLYKLIWERTVASQMKDARYLSTSIDFDNNGYTFRSNGNVVIFDGFTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCEEEECCCEEE
YSLNTKDSELPELKDREAISSNEIEPKQHFTKPKPRYTEASLVKTLEEDGIGRPSTYASI
EECCCCCCCCCCCHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
ISSILSRNYVELKEKKFYTTEIGEKVCEFVVKYFDSIINEKFTAEMENELDNIAENDVEW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHH
KKVIRDFYNGFNEDLVNAKNAQDKFKVKDEPIGRKCPECGNDLVIKHGRNGKFIGCSAFP
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCC
ECKYTETIVKDTKVKCPKCGHRIIEKVSKRGKVFYGCENYPNCDFALWDKPTGEKCPECG
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCCCCCHHH
SLLVHRKNRRGESVLCSNEKCNYKKLEK
HHHHCCCCCCCCCEEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]