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The map label for this gene is thiN [H]

Identifier: 169824368

GI number: 169824368

Start: 734847

End: 735479

Strand: Direct

Name: thiN [H]

Synonym: FMG_0671

Alternate gene names: 169824368

Gene position: 734847-735479 (Clockwise)

Preceding gene: 169824367

Following gene: 169824370

Centisome position: 40.88

GC content: 26.7

Gene sequence:

>633_bases
ATGATTGGGTTGATTTGTTGCGCCGGTGAATTGTCTGATAGGATTCTTTTTGAAGAATATTACGAAAAATCAGATTTTAA
AATAGCAGTAGATGGTGGTACAAAATATTTTACAGACTATGACAAAGATTTTGATTTCGCTATAGGAGATTTTGATTCTA
TAAAAATCGAGGATAAAAAATTTCTAGAAGTAAATAACAAGATTTATGAAAAATATAAGCGAAAAAAAGATTTTACTGAT
TTTGAAGCCGCAATAAATATATTAATAGAAAAAAATTGTAAAACAATATATGCCTTTGGTGCAACAGGAACAAGATTAGA
TCATACAATGTCGAATTTAATCTACTCAAAAAAATGCTTTGATATTGGAATTGAACTTGTATTTGTTGGAAATAATAACA
TTATTAAATTCTTAGGCGAAAGCACTGAATGCGTTATGAGATATGATTATATCTCTATTGTAGTATTATCGAATAATGGC
ATGAGAATTAGATTAAAAGGTTTTGAATACGATAGTGAAAACTTGGATGTGGATTATTGTTCAACATTAACTATTAGCAA
TAAAATAAAAGATAAAAAAGCATATATAGAATTAATTGAAGGATATGGTCTTTTGATTGATTCTAGAGATTAA

Upstream 100 bases:

>100_bases
GATCTTTCTTGTATAATATTACTTATATCGTTCCAAATGGAATTATAGCTATAGTTGTAATTTTAATTTTAATTAAACCT
GTATCAAAGGTAAGTAAGAA

Downstream 100 bases:

>100_bases
AAAAGAGTCGAAATTTCGACTCTTTGTTTTATTCTTCTTCTGTTGTTTTGATAAATTGAGGTCTTTCAACTAAACCTGAT
CTTAAGCATCTTGTGCACAC

Product: putative thiamin pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIGLICCAGELSDRILFEEYYEKSDFKIAVDGGTKYFTDYDKDFDFAIGDFDSIKIEDKKFLEVNNKIYEKYKRKKDFTD
FEAAINILIEKNCKTIYAFGATGTRLDHTMSNLIYSKKCFDIGIELVFVGNNNIIKFLGESTECVMRYDYISIVVLSNNG
MRIRLKGFEYDSENLDVDYCSTLTISNKIKDKKAYIELIEGYGLLIDSRD

Sequences:

>Translated_210_residues
MIGLICCAGELSDRILFEEYYEKSDFKIAVDGGTKYFTDYDKDFDFAIGDFDSIKIEDKKFLEVNNKIYEKYKRKKDFTD
FEAAINILIEKNCKTIYAFGATGTRLDHTMSNLIYSKKCFDIGIELVFVGNNNIIKFLGESTECVMRYDYISIVVLSNNG
MRIRLKGFEYDSENLDVDYCSTLTISNKIKDKKAYIELIEGYGLLIDSRD
>Mature_210_residues
MIGLICCAGELSDRILFEEYYEKSDFKIAVDGGTKYFTDYDKDFDFAIGDFDSIKIEDKKFLEVNNKIYEKYKRKKDFTD
FEAAINILIEKNCKTIYAFGATGTRLDHTMSNLIYSKKCFDIGIELVFVGNNNIIKFLGESTECVMRYDYISIVVLSNNG
MRIRLKGFEYDSENLDVDYCSTLTISNKIKDKKAYIELIEGYGLLIDSRD

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24265; Mature: 24265

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLICCAGELSDRILFEEYYEKSDFKIAVDGGTKYFTDYDKDFDFAIGDFDSIKIEDKK
CEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCEEECCCCCCCCEEECCCCEEEECCHH
FLEVNNKIYEKYKRKKDFTDFEAAINILIEKNCKTIYAFGATGTRLDHTMSNLIYSKKCF
EEHHHHHHHHHHHHCCCCCCHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCEEE
DIGIELVFVGNNNIIKFLGESTECVMRYDYISIVVLSNNGMRIRLKGFEYDSENLDVDYC
ECCEEEEEECCCCEEEEECCCCEEEEEEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEE
STLTISNKIKDKKAYIELIEGYGLLIDSRD
EEEEECCCCCHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure
MIGLICCAGELSDRILFEEYYEKSDFKIAVDGGTKYFTDYDKDFDFAIGDFDSIKIEDKK
CEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCEEECCCCCCCCEEECCCCEEEECCHH
FLEVNNKIYEKYKRKKDFTDFEAAINILIEKNCKTIYAFGATGTRLDHTMSNLIYSKKCF
EEHHHHHHHHHHHHCCCCCCHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCEEE
DIGIELVFVGNNNIIKFLGESTECVMRYDYISIVVLSNNGMRIRLKGFEYDSENLDVDYC
ECCEEEEEECCCCEEEEECCCCEEEEEEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEE
STLTISNKIKDKKAYIELIEGYGLLIDSRD
EEEEECCCCCHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]