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The map label for this gene is smpB [H]

Identifier: 169824350

GI number: 169824350

Start: 717860

End: 718309

Strand: Direct

Name: smpB [H]

Synonym: FMG_0653

Alternate gene names: 169824350

Gene position: 717860-718309 (Clockwise)

Preceding gene: 169824349

Following gene: 169824351

Centisome position: 39.93

GC content: 29.78

Gene sequence:

>450_bases
ATGAGTGAAATAAAATTATTAGCTAACAATAAAAAAGCACGCCACGAGTATTTTATTGAAGAAGTGTACGAATGTGGAAT
TGAATTAAAGGGAACTGAAGTAAAATCTATCAAAAAAGGTAAGGTTTCTATTAAGGAAAGTTATTGTATGATAGAAAACT
CAGAAGTTTTTGTTTATTCAATGAATGTGAGTCCGTACACAGAAGGAAATATTTTTAATGTGGATAGTTTAAGAAAAAGA
AAACTTTTAATGCATAAGTCTGAAATCAGAAAATTAATCGGGAAAACAAAAGAACAAGGTTACACTTTGATACCACTAAG
GGTTTATGCAAAAAATGGACTCGTAAAAGTAGAGATAGCTCTTGCGAAAGGTAAAAATTTGTACGATAAGAGAGAAACTT
TAAAAAAACGTGATGATTTGAGAAATATGCAAAGAGCAATGAGAAGATAG

Upstream 100 bases:

>100_bases
ATCATATCATATTGGACAAAAAGTTAGAGTTAAAGTTATAAATAGCGATAAAGACAAGCGTCAAATAGATTTTGAATTTG
TAAATTAGAAAAGGAATATA

Downstream 100 bases:

>100_bases
GAGTAATAATGAGCTTTGACGGAATTGTAACAAAAGCGATAGTAGATGATATAAAAGAAAAATTAATCAATGGGAAAATC
AGCAAGGTTAATCAACCAGA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSEIKLLANNKKARHEYFIEEVYECGIELKGTEVKSIKKGKVSIKESYCMIENSEVFVYSMNVSPYTEGNIFNVDSLRKR
KLLMHKSEIRKLIGKTKEQGYTLIPLRVYAKNGLVKVEIALAKGKNLYDKRETLKKRDDLRNMQRAMRR

Sequences:

>Translated_149_residues
MSEIKLLANNKKARHEYFIEEVYECGIELKGTEVKSIKKGKVSIKESYCMIENSEVFVYSMNVSPYTEGNIFNVDSLRKR
KLLMHKSEIRKLIGKTKEQGYTLIPLRVYAKNGLVKVEIALAKGKNLYDKRETLKKRDDLRNMQRAMRR
>Mature_148_residues
SEIKLLANNKKARHEYFIEEVYECGIELKGTEVKSIKKGKVSIKESYCMIENSEVFVYSMNVSPYTEGNIFNVDSLRKRK
LLMHKSEIRKLIGKTKEQGYTLIPLRVYAKNGLVKVEIALAKGKNLYDKRETLKKRDDLRNMQRAMRR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=42.8571428571429, Blast_Score=125, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17438; Mature: 17307

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKLLANNKKARHEYFIEEVYECGIELKGTEVKSIKKGKVSIKESYCMIENSEVFVYS
CCCEEEEECCCHHHHHHHHHHHHHCCCEECCCHHCHHHCCCEEEECCEEEEECCEEEEEE
MNVSPYTEGNIFNVDSLRKRKLLMHKSEIRKLIGKTKEQGYTLIPLRVYAKNGLVKVEIA
ECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEEEEECCCEEEEEEE
LAKGKNLYDKRETLKKRDDLRNMQRAMRR
EECCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEIKLLANNKKARHEYFIEEVYECGIELKGTEVKSIKKGKVSIKESYCMIENSEVFVYS
CCEEEEECCCHHHHHHHHHHHHHCCCEECCCHHCHHHCCCEEEECCEEEEECCEEEEEE
MNVSPYTEGNIFNVDSLRKRKLLMHKSEIRKLIGKTKEQGYTLIPLRVYAKNGLVKVEIA
ECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEEEEECCCEEEEEEE
LAKGKNLYDKRETLKKRDDLRNMQRAMRR
EECCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA