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The map label for this gene is proS

Identifier: 169823842

GI number: 169823842

Start: 169888

End: 171618

Strand: Direct

Name: proS

Synonym: FMG_0145

Alternate gene names: 169823842

Gene position: 169888-171618 (Clockwise)

Preceding gene: 169823841

Following gene: 169823843

Centisome position: 9.45

GC content: 36.92

Gene sequence:

>1731_bases
ATGAGAATGAATAAATTTTATATGCCAACTTTAAGAGAGGATCCACAAGATGCAGAAATAGCAAGCCACAAGCTTCTATT
AAGAGCTGGCATGATTAGAAAAACAGCAGCAGGATTGTACAGTTATTTGCCATTGGGATATCGAATTGTAAGAAAGGTAG
AAAACATAGTTCGTGAAGAAATGGACAACTACGGTTCACAAGAAATTCACATGCCAATAACTCAACCTCGTGAAATCTGG
GAAGAATCTGGAAGATGGAAGACATTTGGACCAGAAATGTTCAAGCTCAAAGACAGAAACAACCGTGAATTTTGCCTTGG
ACCAACAGCAGAAGAATATTTTACAGATTTAGTAAAAGGTGAAATCAAAAGCTACAAGCAACTTCCACTTAATATTTACC
AAATCCAAACAAAATACCGTGATGAAAAAAGACCAAGATTTGGAATCAATCGTTCCAGAGAATTCTTGATGCAAGATGCT
TATACATTTGATGTCAACGAAGAAGCAATGGGTGAAGCTTACATGAACATGTGGAGAGCGTATGAAGTTGTATTCAACAG
ACTTGGTTTGGAATACAAAATCGTAGCAGGAGACAGTGGCGCAATGGGTGGTAACAGTTCACACGAATTTATCGCATTAA
GTGATGTAGGTGAAGGTGTGATTTGCTACAGCGATGACAGTGATTTCGCAGCAACAGACGAAAAAGCATACGTCTACTAT
CAAGTAAACGATGAAAATGTAGAAAAACTTTCATCAGAAAAAGTCCTCACACCAAACTGCAAAACAATTGAAGAAGTAAG
TGATTTCTTGAATGTTGACGCAGCACATTGTTTGAAGGCTGTGGATTTGATGGTAGAAGGAAAACCTGTAATAGTGTTCA
TACCAGGAGATCGTGAACTTAACATGTCCAAACTTGTAAGTTATCTGAAATGTCCAGAACACGAAATCGAAATGATGGAA
GAACAAGACATCTTGGCATTGAACAGTTCACCAGGATTTACAGGCCCAATAGGATTAGATTGTAGAATCATAATCGATTC
AAGAGTTAAGCAAATGAAAAACTTCGTCGTTGGAGCGAATGAAGAAAACTATCACATCAAAAATGTAAACTATGGAGATG
ATTTCGAAGGAGAAATCGTGGAAGATTTGTTGATGGTACAAGAAGGAGATATCGATCCTGAAACAAAATCTCCATTGAAA
TTCAAAAGAGGAATCGAAGTTGGAAATATCTTCCAATTGGGACAAAAATATTCCAAGAGCATGAACGCTACATTCCTTGA
TGAAAATGGAAAAGAACAATTCTTCTGGATGGGATCATACGGAATCGGCGTAACAAGAAGTGTCAGCGCAATTGTAGAAC
AAAACCACGACGACAAGGGAATGATTTGGCCATTAGTTGTAGCTCCATATCATGTGATCATCACAATTGTTAACACAAAA
GATGAAGAACAAAACACTCTTGCAGAAAAACTTTATGAAAAATTACTTCTTCAAGGAGTAGAAGTTCTACTTGATGATAG
AAAAGAAAGAGTAGGAGTAAAATTCAACGACCGTGATTTGATAGGAATTCCACTAAGAATAACAGTCGGTAAGAAAGCTG
CTGAAGATATCGTAGAATTTTCTGAAAGAAGAACGCTTGAAAATGTAGAAATGTCATCAACAGAAGCTTACGAGAAAGTA
ATGGAAATCATCAACTCCAACTTGAAATCAGTTGGTGGATTATACAGATAA

Upstream 100 bases:

>100_bases
TAACTAACAAACTAGAAGTGGTATAGCGATCCTTCGTCTTCGTGACGAGGGATCTTTTTTTAGATACATATAACTACTAA
AAGAAAGGAACTTATAAATT

Downstream 100 bases:

>100_bases
TTTAATTTGATATTTAAAACGAAAACTACGGGTGATAAGGCTCGTAGTTTTTTTATTTCAAAAATGTAAAGCATACTTGA
CAAAATATGTAAAGCATACT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MRMNKFYMPTLREDPQDAEIASHKLLLRAGMIRKTAAGLYSYLPLGYRIVRKVENIVREEMDNYGSQEIHMPITQPREIW
EESGRWKTFGPEMFKLKDRNNREFCLGPTAEEYFTDLVKGEIKSYKQLPLNIYQIQTKYRDEKRPRFGINRSREFLMQDA
YTFDVNEEAMGEAYMNMWRAYEVVFNRLGLEYKIVAGDSGAMGGNSSHEFIALSDVGEGVICYSDDSDFAATDEKAYVYY
QVNDENVEKLSSEKVLTPNCKTIEEVSDFLNVDAAHCLKAVDLMVEGKPVIVFIPGDRELNMSKLVSYLKCPEHEIEMME
EQDILALNSSPGFTGPIGLDCRIIIDSRVKQMKNFVVGANEENYHIKNVNYGDDFEGEIVEDLLMVQEGDIDPETKSPLK
FKRGIEVGNIFQLGQKYSKSMNATFLDENGKEQFFWMGSYGIGVTRSVSAIVEQNHDDKGMIWPLVVAPYHVIITIVNTK
DEEQNTLAEKLYEKLLLQGVEVLLDDRKERVGVKFNDRDLIGIPLRITVGKKAAEDIVEFSERRTLENVEMSSTEAYEKV
MEIINSNLKSVGGLYR

Sequences:

>Translated_576_residues
MRMNKFYMPTLREDPQDAEIASHKLLLRAGMIRKTAAGLYSYLPLGYRIVRKVENIVREEMDNYGSQEIHMPITQPREIW
EESGRWKTFGPEMFKLKDRNNREFCLGPTAEEYFTDLVKGEIKSYKQLPLNIYQIQTKYRDEKRPRFGINRSREFLMQDA
YTFDVNEEAMGEAYMNMWRAYEVVFNRLGLEYKIVAGDSGAMGGNSSHEFIALSDVGEGVICYSDDSDFAATDEKAYVYY
QVNDENVEKLSSEKVLTPNCKTIEEVSDFLNVDAAHCLKAVDLMVEGKPVIVFIPGDRELNMSKLVSYLKCPEHEIEMME
EQDILALNSSPGFTGPIGLDCRIIIDSRVKQMKNFVVGANEENYHIKNVNYGDDFEGEIVEDLLMVQEGDIDPETKSPLK
FKRGIEVGNIFQLGQKYSKSMNATFLDENGKEQFFWMGSYGIGVTRSVSAIVEQNHDDKGMIWPLVVAPYHVIITIVNTK
DEEQNTLAEKLYEKLLLQGVEVLLDDRKERVGVKFNDRDLIGIPLRITVGKKAAEDIVEFSERRTLENVEMSSTEAYEKV
MEIINSNLKSVGGLYR
>Mature_576_residues
MRMNKFYMPTLREDPQDAEIASHKLLLRAGMIRKTAAGLYSYLPLGYRIVRKVENIVREEMDNYGSQEIHMPITQPREIW
EESGRWKTFGPEMFKLKDRNNREFCLGPTAEEYFTDLVKGEIKSYKQLPLNIYQIQTKYRDEKRPRFGINRSREFLMQDA
YTFDVNEEAMGEAYMNMWRAYEVVFNRLGLEYKIVAGDSGAMGGNSSHEFIALSDVGEGVICYSDDSDFAATDEKAYVYY
QVNDENVEKLSSEKVLTPNCKTIEEVSDFLNVDAAHCLKAVDLMVEGKPVIVFIPGDRELNMSKLVSYLKCPEHEIEMME
EQDILALNSSPGFTGPIGLDCRIIIDSRVKQMKNFVVGANEENYHIKNVNYGDDFEGEIVEDLLMVQEGDIDPETKSPLK
FKRGIEVGNIFQLGQKYSKSMNATFLDENGKEQFFWMGSYGIGVTRSVSAIVEQNHDDKGMIWPLVVAPYHVIITIVNTK
DEEQNTLAEKLYEKLLLQGVEVLLDDRKERVGVKFNDRDLIGIPLRITVGKKAAEDIVEFSERRTLENVEMSSTEAYEKV
MEIINSNLKSVGGLYR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=253, Percent_Identity=37.9446640316205, Blast_Score=195, Evalue=1e-49,
Organism=Escherichia coli, GI1786392, Length=553, Percent_Identity=45.750452079566, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1788013, Length=165, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115532348, Length=215, Percent_Identity=39.0697674418605, Blast_Score=165, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=91, Percent_Identity=40.6593406593407, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71991342, Length=191, Percent_Identity=31.413612565445, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71991346, Length=134, Percent_Identity=35.0746268656716, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=554, Percent_Identity=31.2274368231047, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322654, Length=188, Percent_Identity=26.063829787234, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656200, Length=306, Percent_Identity=31.6993464052288, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_FINM2 (B0RZJ0)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691453.1
- ProteinModelPortal:   B0RZJ0
- SMR:   B0RZJ0
- GeneID:   6018820
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0145
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   FMAG334413:FMG_0145-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 65990; Mature: 65990

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMNKFYMPTLREDPQDAEIASHKLLLRAGMIRKTAAGLYSYLPLGYRIVRKVENIVREE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDNYGSQEIHMPITQPREIWEESGRWKTFGPEMFKLKDRNNREFCLGPTAEEYFTDLVKG
HHCCCCCEEEECCCCHHHHHHHCCCEEECCCCEEEEECCCCCEEEECCCHHHHHHHHHHH
EIKSYKQLPLNIYQIQTKYRDEKRPRFGINRSREFLMQDAYTFDVNEEAMGEAYMNMWRA
HHHHHHCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHCCEEECCCHHHHHHHHHHHHHH
YEVVFNRLGLEYKIVAGDSGAMGGNSSHEFIALSDVGEGVICYSDDSDFAATDEKAYVYY
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEE
QVNDENVEKLSSEKVLTPNCKTIEEVSDFLNVDAAHCLKAVDLMVEGKPVIVFIPGDREL
EECCHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCCEEEEEECCCCCC
NMSKLVSYLKCPEHEIEMMEEQDILALNSSPGFTGPIGLDCRIIIDSRVKQMKNFVVGAN
CHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHEECCC
EENYHIKNVNYGDDFEGEIVEDLLMVQEGDIDPETKSPLKFKRGIEVGNIFQLGQKYSKS
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHC
MNATFLDENGKEQFFWMGSYGIGVTRSVSAIVEQNHDDKGMIWPLVVAPYHVIITIVNTK
CCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEHHHHCCEEEEEEEECCC
DEEQNTLAEKLYEKLLLQGVEVLLDDRKERVGVKFNDRDLIGIPLRITVGKKAAEDIVEF
CCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEECCCCEEEEEEEEEECCHHHHHHHHH
SERRTLENVEMSSTEAYEKVMEIINSNLKSVGGLYR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRMNKFYMPTLREDPQDAEIASHKLLLRAGMIRKTAAGLYSYLPLGYRIVRKVENIVREE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDNYGSQEIHMPITQPREIWEESGRWKTFGPEMFKLKDRNNREFCLGPTAEEYFTDLVKG
HHCCCCCEEEECCCCHHHHHHHCCCEEECCCCEEEEECCCCCEEEECCCHHHHHHHHHHH
EIKSYKQLPLNIYQIQTKYRDEKRPRFGINRSREFLMQDAYTFDVNEEAMGEAYMNMWRA
HHHHHHCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHCCEEECCCHHHHHHHHHHHHHH
YEVVFNRLGLEYKIVAGDSGAMGGNSSHEFIALSDVGEGVICYSDDSDFAATDEKAYVYY
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEE
QVNDENVEKLSSEKVLTPNCKTIEEVSDFLNVDAAHCLKAVDLMVEGKPVIVFIPGDREL
EECCHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCCEEEEEECCCCCC
NMSKLVSYLKCPEHEIEMMEEQDILALNSSPGFTGPIGLDCRIIIDSRVKQMKNFVVGAN
CHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHEECCC
EENYHIKNVNYGDDFEGEIVEDLLMVQEGDIDPETKSPLKFKRGIEVGNIFQLGQKYSKS
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHC
MNATFLDENGKEQFFWMGSYGIGVTRSVSAIVEQNHDDKGMIWPLVVAPYHVIITIVNTK
CCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEHHHHCCEEEEEEEECCC
DEEQNTLAEKLYEKLLLQGVEVLLDDRKERVGVKFNDRDLIGIPLRITVGKKAAEDIVEF
CCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEECCCCEEEEEEEEEECCHHHHHHHHH
SERRTLENVEMSSTEAYEKVMEIINSNLKSVGGLYR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA