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The map label for this gene is purA

Identifier: 169823833

GI number: 169823833

Start: 157935

End: 159191

Strand: Direct

Name: purA

Synonym: FMG_0136

Alternate gene names: 169823833

Gene position: 157935-159191 (Clockwise)

Preceding gene: 169823832

Following gene: 169823834

Centisome position: 8.79

GC content: 34.45

Gene sequence:

>1257_bases
ATGAATAAATTAGCGATAATTGGCGCACAATGGGGAGACGAAGGTAAGGGAAAGGTTGTAAATTATTTTTCTCAATTTAA
TGATATTATTGTAAGATCTTCTGGTGGAGCCAACGCAGGACATACTATTTATTTTAAAGACAAAAAATACGTACATCATT
TACTACCATCTATAACTTTTGACACAGATTCTAAGGGATTTTTGGCAAAAGGAATGGTAATCGAATTAGAACAAATGATT
GAAGAGTTGAAAGTTTTGGAAGCTGATTTCCCAGGAATTTCCAAGAGATTCATGGTCGATGTAGAAACATTTATCGTTCT
ACCTTACCATAAAGAAGAAGATGGATTGTTGGAATCTATGAGAAAAAATCCTATCGGAACTACAAAAAGAGGTATCGGAC
CTGCTTATCAAGACAAAGCTGCAAGACAAAACGTTAGAATTATTGATTTGTTTGACGATGAATTGTTAAGAGAACGCGTT
GAAGAAATAGTTTATTTGAAAAACAACATCTACGAAGGCAAAATGCACATCGATGTCGATGAAACAGTAAATTATTTGCT
TGACAAATTCGACCAATTACTAAAATTAGGCGTTACATTCACATCAGCATCTGAAATTGAAGATGAAATCAAAAACAAAA
GAGTTTTGTTTGAAGGCGCACAAGGAATCATGCTTGATTTGGACTCAGGAACATATCCATACGTTACATCAAGCACTACA
ACTGCCTACAGCGCATCTTGTGCAGATGTTACATTGACTGATGATGACACAATCATGGGAGTTGTAAAAGCATACACATC
CAGAGTTGGAGAAGGAGAATTCCCAACAGAATTGTTCGATGAAGAAGCTGACAAGCTTAGAAAATTAGGCAATGAATTCG
GTGCTACAACTGGAAGAAATAGACGTGTAGGTTGGATTGACCTTGCACAATTACGTTATGCAATCAAAAAGAGCAAAATC
AACTCAATCGTCATGACAAAAGCAGACGTATTGAACGGCTACGATAAAGTAAAAGTATGCGTTGGATATGAAATAGATGG
AGTTGAACAAAAAATGCCATTCATTTCTAATGATTTCAAAAAAGCAAAACCAATCTACAAAGAATTTGACGGTTGGAACG
ATGTGTTTGAAGTAAACTTCTTGAAATACATGACATTTATCGAAAAAGAATTAGACACAGAAATCTCTTACGTTTCTTAT
GGACCAAAAACAGAAGAAATTATGGAAAAAAGAGATTTTATCATGAATATAAAATAA

Upstream 100 bases:

>100_bases
TCATACTATTTTTAAATGGCGGAATATGGTATAATTAGTAGTTGTGATTTGTAGTTAACTTAACAGTTAAATGAATCTAA
TAATTTGAAAGGAAGTTGTT

Downstream 100 bases:

>100_bases
AAGGAGATATCCGCAGTTTAAAAGACTGCGGATTTTTTTAAATGGATACAGTAAATTTATTTATCGAATATGTAAAATTA
AATATCAATAAGACATACGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNKLAIIGAQWGDEGKGKVVNYFSQFNDIIVRSSGGANAGHTIYFKDKKYVHHLLPSITFDTDSKGFLAKGMVIELEQMI
EELKVLEADFPGISKRFMVDVETFIVLPYHKEEDGLLESMRKNPIGTTKRGIGPAYQDKAARQNVRIIDLFDDELLRERV
EEIVYLKNNIYEGKMHIDVDETVNYLLDKFDQLLKLGVTFTSASEIEDEIKNKRVLFEGAQGIMLDLDSGTYPYVTSSTT
TAYSASCADVTLTDDDTIMGVVKAYTSRVGEGEFPTELFDEEADKLRKLGNEFGATTGRNRRVGWIDLAQLRYAIKKSKI
NSIVMTKADVLNGYDKVKVCVGYEIDGVEQKMPFISNDFKKAKPIYKEFDGWNDVFEVNFLKYMTFIEKELDTEISYVSY
GPKTEEIMEKRDFIMNIK

Sequences:

>Translated_418_residues
MNKLAIIGAQWGDEGKGKVVNYFSQFNDIIVRSSGGANAGHTIYFKDKKYVHHLLPSITFDTDSKGFLAKGMVIELEQMI
EELKVLEADFPGISKRFMVDVETFIVLPYHKEEDGLLESMRKNPIGTTKRGIGPAYQDKAARQNVRIIDLFDDELLRERV
EEIVYLKNNIYEGKMHIDVDETVNYLLDKFDQLLKLGVTFTSASEIEDEIKNKRVLFEGAQGIMLDLDSGTYPYVTSSTT
TAYSASCADVTLTDDDTIMGVVKAYTSRVGEGEFPTELFDEEADKLRKLGNEFGATTGRNRRVGWIDLAQLRYAIKKSKI
NSIVMTKADVLNGYDKVKVCVGYEIDGVEQKMPFISNDFKKAKPIYKEFDGWNDVFEVNFLKYMTFIEKELDTEISYVSY
GPKTEEIMEKRDFIMNIK
>Mature_418_residues
MNKLAIIGAQWGDEGKGKVVNYFSQFNDIIVRSSGGANAGHTIYFKDKKYVHHLLPSITFDTDSKGFLAKGMVIELEQMI
EELKVLEADFPGISKRFMVDVETFIVLPYHKEEDGLLESMRKNPIGTTKRGIGPAYQDKAARQNVRIIDLFDDELLRERV
EEIVYLKNNIYEGKMHIDVDETVNYLLDKFDQLLKLGVTFTSASEIEDEIKNKRVLFEGAQGIMLDLDSGTYPYVTSSTT
TAYSASCADVTLTDDDTIMGVVKAYTSRVGEGEFPTELFDEEADKLRKLGNEFGATTGRNRRVGWIDLAQLRYAIKKSKI
NSIVMTKADVLNGYDKVKVCVGYEIDGVEQKMPFISNDFKKAKPIYKEFDGWNDVFEVNFLKYMTFIEKELDTEISYVSY
GPKTEEIMEKRDFIMNIK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=36.6589327146172, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=34.6604215456674, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=33.3333333333333, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=38.8372093023256, Blast_Score=288, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=38.0281690140845, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=37.9391100702576, Blast_Score=261, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=35.981308411215, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=33.8862559241706, Blast_Score=222, Evalue=4e-58,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_FINM2 (B0RZL3)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691444.1
- ProteinModelPortal:   B0RZL3
- SMR:   B0RZL3
- GeneID:   6020143
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0136
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK13783
- BioCyc:   FMAG334413:FMG_0136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47596; Mature: 47596

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 221-221 BINDING 236-236 BINDING 299-299 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLAIIGAQWGDEGKGKVVNYFSQFNDIIVRSSGGANAGHTIYFKDKKYVHHLLPSITF
CCCEEEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHCCCCEE
DTDSKGFLAKGMVIELEQMIEELKVLEADFPGISKRFMVDVETFIVLPYHKEEDGLLESM
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
RKNPIGTTKRGIGPAYQDKAARQNVRIIDLFDDELLRERVEEIVYLKNNIYEGKMHIDVD
HCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCEECCEEEEEHH
ETVNYLLDKFDQLLKLGVTFTSASEIEDEIKNKRVLFEGAQGIMLDLDSGTYPYVTSSTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCEEEEEECCCCCCEEECCCC
TAYSASCADVTLTDDDTIMGVVKAYTSRVGEGEFPTELFDEEADKLRKLGNEFGATTGRN
CEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
RRVGWIDLAQLRYAIKKSKINSIVMTKADVLNGYDKVKVCVGYEIDGVEQKMPFISNDFK
CEECCEEHHHHHHHHHHHHCCCEEEEHHHHHCCCCEEEEEEEEEECCHHHHCCCHHCCHH
KAKPIYKEFDGWNDVFEVNFLKYMTFIEKELDTEISYVSYGPKTEEIMEKRDFIMNIK
HHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHEECCC
>Mature Secondary Structure
MNKLAIIGAQWGDEGKGKVVNYFSQFNDIIVRSSGGANAGHTIYFKDKKYVHHLLPSITF
CCCEEEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHCCCCEE
DTDSKGFLAKGMVIELEQMIEELKVLEADFPGISKRFMVDVETFIVLPYHKEEDGLLESM
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
RKNPIGTTKRGIGPAYQDKAARQNVRIIDLFDDELLRERVEEIVYLKNNIYEGKMHIDVD
HCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCEECCEEEEEHH
ETVNYLLDKFDQLLKLGVTFTSASEIEDEIKNKRVLFEGAQGIMLDLDSGTYPYVTSSTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCEEEEEECCCCCCEEECCCC
TAYSASCADVTLTDDDTIMGVVKAYTSRVGEGEFPTELFDEEADKLRKLGNEFGATTGRN
CEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
RRVGWIDLAQLRYAIKKSKINSIVMTKADVLNGYDKVKVCVGYEIDGVEQKMPFISNDFK
CEECCEEHHHHHHHHHHHHCCCEEEEHHHHHCCCCEEEEEEEEEECCHHHHCCCHHCCHH
KAKPIYKEFDGWNDVFEVNFLKYMTFIEKELDTEISYVSYGPKTEEIMEKRDFIMNIK
HHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA