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The map label for this gene is birA [H]

Identifier: 169823748

GI number: 169823748

Start: 69050

End: 69778

Strand: Direct

Name: birA [H]

Synonym: FMG_0051

Alternate gene names: 169823748

Gene position: 69050-69778 (Clockwise)

Preceding gene: 169823747

Following gene: 169823750

Centisome position: 3.84

GC content: 33.74

Gene sequence:

>729_bases
ATGGATACGGAAAAAATCAAGAAACAAACTGGAATAAATAAGGTGATTTTCAAAAAGGAAGTCACATCGACAAATGATGA
AATTCTGAATGAAAAGCAAGACGATACGTTGATGATTGCTTTTTTCCAATCGAAGGGTAAGGGAAGATATGGAAGAAAGT
TTTTCTCGCCAGAAAATGGGTTGTATTTTTCGTATTTGGTAAATGAAGAAATGACATTTCAACAATCGCAATTTATCACA
CCAATTATGGGAATTGCTGTAAGCTATGCAATTGACGAGGTGTTCGGCGTGAATACGTCTATTAAATGGGTCAATGATAT
TCTGCTTGAAAAAAAGAAAATCTGCGGCATACTCACAGAATGCAAGGTGGAGAAAAATATCTGCCACGATATTGTGGTTG
GTGTTGGAATTAACGTCTACCAAACAGATTTTCCAGAAGATATAAAAGATACGGCGGGATTCATTGAAGAGAAAGATGAT
ACAGATAAAAGAGAAGATTTGGTAATAAAAATAATGCAGAATTTCAAAAGATTTTGGAGCGAGGATAGAGATAACTTGGT
AGAAAAATATAGAAACAAAGCTACGTTCATTGGCAATGAAATCAAAGTTCACAAAAACGGTGATTATGTAAAAGCTGTTG
CAATGAATATCAACGACGATTTTAGCTTGAAAGTAAAATACGACGACGGAACAATTGAAGATTTAAATATAGGAGAGATT
TTTTTATAA

Upstream 100 bases:

>100_bases
AGTATGAAATTATCGCTGTTTTGAAGCAAAGACTAGAAGATGATGACAAGGAAATGAATAAAAAAGAACGTAAAAAATGG
GATAGATGGGATGAAGGTTA

Downstream 100 bases:

>100_bases
AATCTCTTTTTTTTATTGGTCTATTTCCTTCCAAGCATCATCATGATCGACTAATTCGATTTTTTTGTTTTTCTTGGATT
CTTCGTATTCTTTTATTACT

Product: putative biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDTEKIKKQTGINKVIFKKEVTSTNDEILNEKQDDTLMIAFFQSKGKGRYGRKFFSPENGLYFSYLVNEEMTFQQSQFIT
PIMGIAVSYAIDEVFGVNTSIKWVNDILLEKKKICGILTECKVEKNICHDIVVGVGINVYQTDFPEDIKDTAGFIEEKDD
TDKREDLVIKIMQNFKRFWSEDRDNLVEKYRNKATFIGNEIKVHKNGDYVKAVAMNINDDFSLKVKYDDGTIEDLNIGEI
FL

Sequences:

>Translated_242_residues
MDTEKIKKQTGINKVIFKKEVTSTNDEILNEKQDDTLMIAFFQSKGKGRYGRKFFSPENGLYFSYLVNEEMTFQQSQFIT
PIMGIAVSYAIDEVFGVNTSIKWVNDILLEKKKICGILTECKVEKNICHDIVVGVGINVYQTDFPEDIKDTAGFIEEKDD
TDKREDLVIKIMQNFKRFWSEDRDNLVEKYRNKATFIGNEIKVHKNGDYVKAVAMNINDDFSLKVKYDDGTIEDLNIGEI
FL
>Mature_242_residues
MDTEKIKKQTGINKVIFKKEVTSTNDEILNEKQDDTLMIAFFQSKGKGRYGRKFFSPENGLYFSYLVNEEMTFQQSQFIT
PIMGIAVSYAIDEVFGVNTSIKWVNDILLEKKKICGILTECKVEKNICHDIVVGVGINVYQTDFPEDIKDTAGFIEEKDD
TDKREDLVIKIMQNFKRFWSEDRDNLVEKYRNKATFIGNEIKVHKNGDYVKAVAMNINDDFSLKVKYDDGTIEDLNIGEI
FL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=251, Percent_Identity=23.9043824701195, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27955; Mature: 27955

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTEKIKKQTGINKVIFKKEVTSTNDEILNEKQDDTLMIAFFQSKGKGRYGRKFFSPENG
CCHHHHHHHCCCCCEEEEHHCCCCCHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
LYFSYLVNEEMTFQQSQFITPIMGIAVSYAIDEVFGVNTSIKWVNDILLEKKKICGILTE
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
CKVEKNICHDIVVGVGINVYQTDFPEDIKDTAGFIEEKDDTDKREDLVIKIMQNFKRFWS
HHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
EDRDNLVEKYRNKATFIGNEIKVHKNGDYVKAVAMNINDDFSLKVKYDDGTIEDLNIGEI
CCHHHHHHHHCCCCEEECCEEEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCE
FL
EC
>Mature Secondary Structure
MDTEKIKKQTGINKVIFKKEVTSTNDEILNEKQDDTLMIAFFQSKGKGRYGRKFFSPENG
CCHHHHHHHCCCCCEEEEHHCCCCCHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
LYFSYLVNEEMTFQQSQFITPIMGIAVSYAIDEVFGVNTSIKWVNDILLEKKKICGILTE
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
CKVEKNICHDIVVGVGINVYQTDFPEDIKDTAGFIEEKDDTDKREDLVIKIMQNFKRFWS
HHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
EDRDNLVEKYRNKATFIGNEIKVHKNGDYVKAVAMNINDDFSLKVKYDDGTIEDLNIGEI
CCHHHHHHHHCCCCEEECCEEEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCE
FL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]