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The map label for this gene is gyrB [H]

Identifier: 169823702

GI number: 169823702

Start: 4723

End: 6639

Strand: Direct

Name: gyrB [H]

Synonym: FMG_0005

Alternate gene names: 169823702

Gene position: 4723-6639 (Clockwise)

Preceding gene: 169823701

Following gene: 169823703

Centisome position: 0.26

GC content: 38.45

Gene sequence:

>1917_bases
ATGAAGAAAAATAGAGAATATACTGCAGATCAGATTACAGTATTAGAAGGATTGGAACCGGTTCGTGTCAGACCAGGGAT
GTATATCGGTTCTACAGATGAAAAAGGTCTACACCATTTGGTTTACGAAGTAGTCGACAACTCGATTGACGAGGCGTTGG
CTGGCGTGTGTGATTTGATTAAGGTTACTATTAAAAAAGACAATTCCATTATCGTAGAAGATGACGGTTCTGGTATTCCT
GTTGAAACACACCCAAAAACAGGTAAATCAACTCTTGAAACTGTACTTACAGTGCTTCACGCAGGTGGTAAATTTTCAAA
CGATGCTTATCAATACTCAGGTGGTCTTCACGGAGTTGGGGTAAGTTGTGTAAATGCTTTAAGTACATACCTTATTGCCA
GAGTAAAAAGAGACGGCAAGATGTATGAGCAAAGATTTGAAAAAGGAAAAACTGTAACAGGACTTACTGTTGTAAAAGAA
AATGTCAGAGGAACTGGAACTACGATTGAATTCATGCCGGATGATACGATTTTTGAAACGTTGAATTTCGACAAGAATGT
TCTTGCAAAAAGATTCAGAGAAATGGCGTTTTTGAATAAGGGCGTGAAGATTGAATTTTCTGATGAAAGAGACGGCTACA
AGGAATTGTTCCACTACGAAGGTGGTATCAAATCATTTGTAGAATACATCAACAAATCGAAAAAGCCTTTGGATCACGAG
GTAATGTATATCGAAGGAGAAAAGGACACTACAAAAGTTGAAATAGCTATGCAATACACTGAAAGCTACAACGAAACGAT
AGTTTCCTTTACTAACAACATCAAAACAGTTGACGGCGGAACGCATGTTGTAGGATTCAAATCCGCACTTACGCGTGCAC
TTAACGATTATGCAAGAAACAAAAATATATTAAAAGAAAAAGACGATAATTTGTCTGGAGATGATGTCAGAGAAGGTATC
ACTGCGGTTGTTTCAGTAAAACTAAACAATCCACAATTCGAAGGACAAACTAAAGGAAAATTGGGTAACTCAGAAGCTAG
AACTGTTGTAGACAGCGTTGTGTATGAGCATTTGAGTTTTTATTTTGAAGAAAATCCAAAAATCGTCAGAACAATTATCC
AAAAAGCATTGGCATCACAAAAAGCAAGACTTGCAGCCAGACGTGCGAGGGATTTGGTTCGTAAAAAATCAGTGCTTGAC
AATACGACACTTCCTGGAAAACTTGCCGACTGCCAAAGCTCAGACATCGAATACAACGAAATCTTCCTTGTCGAAGGGGA
TTCTGCCGGTGGTTCTGCAAAAGGTGGCAGGGACAACAAATTCCAAGCTATTTTGCCATTGAGAGGTAAAATCTTGAATG
TAGAAAAAGCTCACCTTGAAAGAGTTCTAAATTCAGAAGAAATCAAAGCGATGATTACTGCATTTGGAACGGGAATCGGC
AAGAACTTCGACATCAGTAAACTTCGTTACGGAAAAATCGTAATAATGACCGATGCCGACGTCGACGGAGCGCATATTAG
AACTCTATTACTTACATTCTTGTACAGATTTATGAAGCCATTAGTGGAAAAAGGTCACGTATACATCGCACAACCACCAC
TTTACGGAATCATCAAAGGTGTTAAGGTAATGGAATATTGCTACACAGAACAAGAATTGGAAGAAGCGTTGGAAAAATAC
GGTGAAAGAAGTAACATCAAAGTTCAAAGATACAAAGGTCTTGGTGAAATGAACGCAGAACAATTGTGGGATACTACAAT
GAATCCTGAACACAGAATTCTTATCAAAGTTGAAATCGACGGTGAAGACGAGAACGAACTTGACAACACATTCGATGTTT
TGATGGGAGACAATGTAGAACCAAGAAGAGAATTTATAGAACAAAACGCTGTTTATGCACAAAACATTGACGCTTAG

Upstream 100 bases:

>100_bases
AGAGCATTACTATAAGGTCTATAGTCATAACTAATAGAGGAGTTTACAAGTCAAATATCAGTGGCAATTATTTGATGAAT
AACTTTATGAAAGGAATATA

Downstream 100 bases:

>100_bases
GAGAATAATATGACAGAAAGAAAACTCAATATAAAACCAGCTGATTTGAAAAGAGAAATGGAAAGTGCATATTTGGATTA
TTCTATGAGTGTAATTGTAT

Product: DNA gyrase B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MKKNREYTADQITVLEGLEPVRVRPGMYIGSTDEKGLHHLVYEVVDNSIDEALAGVCDLIKVTIKKDNSIIVEDDGSGIP
VETHPKTGKSTLETVLTVLHAGGKFSNDAYQYSGGLHGVGVSCVNALSTYLIARVKRDGKMYEQRFEKGKTVTGLTVVKE
NVRGTGTTIEFMPDDTIFETLNFDKNVLAKRFREMAFLNKGVKIEFSDERDGYKELFHYEGGIKSFVEYINKSKKPLDHE
VMYIEGEKDTTKVEIAMQYTESYNETIVSFTNNIKTVDGGTHVVGFKSALTRALNDYARNKNILKEKDDNLSGDDVREGI
TAVVSVKLNNPQFEGQTKGKLGNSEARTVVDSVVYEHLSFYFEENPKIVRTIIQKALASQKARLAARRARDLVRKKSVLD
NTTLPGKLADCQSSDIEYNEIFLVEGDSAGGSAKGGRDNKFQAILPLRGKILNVEKAHLERVLNSEEIKAMITAFGTGIG
KNFDISKLRYGKIVIMTDADVDGAHIRTLLLTFLYRFMKPLVEKGHVYIAQPPLYGIIKGVKVMEYCYTEQELEEALEKY
GERSNIKVQRYKGLGEMNAEQLWDTTMNPEHRILIKVEIDGEDENELDNTFDVLMGDNVEPRREFIEQNAVYAQNIDA

Sequences:

>Translated_638_residues
MKKNREYTADQITVLEGLEPVRVRPGMYIGSTDEKGLHHLVYEVVDNSIDEALAGVCDLIKVTIKKDNSIIVEDDGSGIP
VETHPKTGKSTLETVLTVLHAGGKFSNDAYQYSGGLHGVGVSCVNALSTYLIARVKRDGKMYEQRFEKGKTVTGLTVVKE
NVRGTGTTIEFMPDDTIFETLNFDKNVLAKRFREMAFLNKGVKIEFSDERDGYKELFHYEGGIKSFVEYINKSKKPLDHE
VMYIEGEKDTTKVEIAMQYTESYNETIVSFTNNIKTVDGGTHVVGFKSALTRALNDYARNKNILKEKDDNLSGDDVREGI
TAVVSVKLNNPQFEGQTKGKLGNSEARTVVDSVVYEHLSFYFEENPKIVRTIIQKALASQKARLAARRARDLVRKKSVLD
NTTLPGKLADCQSSDIEYNEIFLVEGDSAGGSAKGGRDNKFQAILPLRGKILNVEKAHLERVLNSEEIKAMITAFGTGIG
KNFDISKLRYGKIVIMTDADVDGAHIRTLLLTFLYRFMKPLVEKGHVYIAQPPLYGIIKGVKVMEYCYTEQELEEALEKY
GERSNIKVQRYKGLGEMNAEQLWDTTMNPEHRILIKVEIDGEDENELDNTFDVLMGDNVEPRREFIEQNAVYAQNIDA
>Mature_638_residues
MKKNREYTADQITVLEGLEPVRVRPGMYIGSTDEKGLHHLVYEVVDNSIDEALAGVCDLIKVTIKKDNSIIVEDDGSGIP
VETHPKTGKSTLETVLTVLHAGGKFSNDAYQYSGGLHGVGVSCVNALSTYLIARVKRDGKMYEQRFEKGKTVTGLTVVKE
NVRGTGTTIEFMPDDTIFETLNFDKNVLAKRFREMAFLNKGVKIEFSDERDGYKELFHYEGGIKSFVEYINKSKKPLDHE
VMYIEGEKDTTKVEIAMQYTESYNETIVSFTNNIKTVDGGTHVVGFKSALTRALNDYARNKNILKEKDDNLSGDDVREGI
TAVVSVKLNNPQFEGQTKGKLGNSEARTVVDSVVYEHLSFYFEENPKIVRTIIQKALASQKARLAARRARDLVRKKSVLD
NTTLPGKLADCQSSDIEYNEIFLVEGDSAGGSAKGGRDNKFQAILPLRGKILNVEKAHLERVLNSEEIKAMITAFGTGIG
KNFDISKLRYGKIVIMTDADVDGAHIRTLLLTFLYRFMKPLVEKGHVYIAQPPLYGIIKGVKVMEYCYTEQELEEALEKY
GERSNIKVQRYKGLGEMNAEQLWDTTMNPEHRILIKVEIDGEDENELDNTFDVLMGDNVEPRREFIEQNAVYAQNIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=606, Percent_Identity=26.2376237623762, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=25.0398724082935, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI48994957, Length=537, Percent_Identity=54.0037243947858, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=41.6139240506329, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=658, Percent_Identity=25.3799392097264, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI212645845, Length=606, Percent_Identity=25.4125412541254, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=170, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=679, Percent_Identity=24.8895434462445, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17136538, Length=638, Percent_Identity=25.8620689655172, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71650; Mature: 71650

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNREYTADQITVLEGLEPVRVRPGMYIGSTDEKGLHHLVYEVVDNSIDEALAGVCDLI
CCCCCCCCHHHEEHHCCCCCEEECCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHH
KVTIKKDNSIIVEDDGSGIPVETHPKTGKSTLETVLTVLHAGGKFSNDAYQYSGGLHGVG
HEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHH
VSCVNALSTYLIARVKRDGKMYEQRFEKGKTVTGLTVVKENVRGTGTTIEFMPDDTIFET
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHEEHHCCCCCCCEEEECCCCHHHHH
LNFDKNVLAKRFREMAFLNKGVKIEFSDERDGYKELFHYEGGIKSFVEYINKSKKPLDHE
HCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCE
VMYIEGEKDTTKVEIAMQYTESYNETIVSFTNNIKTVDGGTHVVGFKSALTRALNDYARN
EEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHC
KNILKEKDDNLSGDDVREGITAVVSVKLNNPQFEGQTKGKLGNSEARTVVDSVVYEHLSF
CCCHHCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YFEENPKIVRTIIQKALASQKARLAARRARDLVRKKSVLDNTTLPGKLADCQSSDIEYNE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
IFLVEGDSAGGSAKGGRDNKFQAILPLRGKILNVEKAHLERVLNSEEIKAMITAFGTGIG
EEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHCHHHHHHHHHHHHCCCC
KNFDISKLRYGKIVIMTDADVDGAHIRTLLLTFLYRFMKPLVEKGHVYIAQPPLYGIIKG
CCCCCCEEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
VKVMEYCYTEQELEEALEKYGERSNIKVQRYKGLGEMNAEQLWDTTMNPEHRILIKVEID
HHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCCCEEEEEEEEEC
GEDENELDNTFDVLMGDNVEPRREFIEQNAVYAQNIDA
CCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKNREYTADQITVLEGLEPVRVRPGMYIGSTDEKGLHHLVYEVVDNSIDEALAGVCDLI
CCCCCCCCHHHEEHHCCCCCEEECCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHH
KVTIKKDNSIIVEDDGSGIPVETHPKTGKSTLETVLTVLHAGGKFSNDAYQYSGGLHGVG
HEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHH
VSCVNALSTYLIARVKRDGKMYEQRFEKGKTVTGLTVVKENVRGTGTTIEFMPDDTIFET
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHEEHHCCCCCCCEEEECCCCHHHHH
LNFDKNVLAKRFREMAFLNKGVKIEFSDERDGYKELFHYEGGIKSFVEYINKSKKPLDHE
HCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCE
VMYIEGEKDTTKVEIAMQYTESYNETIVSFTNNIKTVDGGTHVVGFKSALTRALNDYARN
EEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHC
KNILKEKDDNLSGDDVREGITAVVSVKLNNPQFEGQTKGKLGNSEARTVVDSVVYEHLSF
CCCHHCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YFEENPKIVRTIIQKALASQKARLAARRARDLVRKKSVLDNTTLPGKLADCQSSDIEYNE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
IFLVEGDSAGGSAKGGRDNKFQAILPLRGKILNVEKAHLERVLNSEEIKAMITAFGTGIG
EEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHCHHHHHHHHHHHHCCCC
KNFDISKLRYGKIVIMTDADVDGAHIRTLLLTFLYRFMKPLVEKGHVYIAQPPLYGIIKG
CCCCCCEEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
VKVMEYCYTEQELEEALEKYGERSNIKVQRYKGLGEMNAEQLWDTTMNPEHRILIKVEID
HHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCCCEEEEEEEEEC
GEDENELDNTFDVLMGDNVEPRREFIEQNAVYAQNIDA
CCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]