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The map label for this gene is recF [H]

Identifier: 169823701

GI number: 169823701

Start: 3412

End: 4479

Strand: Direct

Name: recF [H]

Synonym: FMG_0004

Alternate gene names: 169823701

Gene position: 3412-4479 (Clockwise)

Preceding gene: 169823700

Following gene: 169823702

Centisome position: 0.19

GC content: 32.58

Gene sequence:

>1068_bases
ATGATCGTTCAAAAATTAAAATTATATAATTACAGGAATTTCTGTGAAATAGAATTGGATTTTTGTGATGGTCTAAATCT
TATTGTAGGCAGAAATGCCTCGGGTAAGACTAATATTTTGGAAGCTATTAATGTAGCTTTGAAGGGAAAAAGTTTCAAAA
CCAATACCAATTCTCATTTGATTAAGTTCGGTGAAGATGAGGCTCGTATTGTCATGGATGTCTACGATGATGGTTTTGAG
GATAAGATAGATATTACTATTAAAAGTAACGAAAAGATACTCAATGTGAACGAAGCTTTTGTGAATACTATCAAAGAATA
CAACGAATATTTTGAGTGTATTGTGTTTAAGCCAGACGATTTGAAGATAATTAAAGAGTCGAAATCGTTGAGAAGAAAAT
TCTTGGATGAGTCCATAAGTGGTGTGGACAATTATTACAGGACTGTTTTGAAACAATACAATCAAGTGTTGGATGAGAGA
AACAAGCTCATCAAAAACCACAGATACAATTCGTATTTTAATGAGCAGTTGAAGGCGTTGAATATTCAATTATCAGATTT
TGGTTCATACATTATGCACAAGAGAAAAAGTTATGTCGAAAGACTTCATCTTATCGCAAAAAATGTGTGCAAGAATTTGA
GTGATGAGAATGACGATTTGTACATGGAAAATAAATTTTCGATTAGATATGTAGAGGATATGACTGATCAAAAAAACACT
TATTACAAGAGTTTGAGAGATATTTTGGAAAAGGACTTGGAAAATAAGCAAACTAATTTAGGTATCCATCGTGATGATTT
GGATATTTTAATCAACGACAAACAGGCTAAGTTTTTTGCATCTCAGGCTCAGGTGAGAACTGCTATTTTATCGATGAAGC
TTGCGCAATTGGATTTGAGTAGATTTTATAATGATAGAATGCCAATTATTTTGTTGGATGATGTGTTCAGTGAACTTGAC
GATTACAGAATCAACTACATCATCAGATATATCAAGGATTTCCAAGCTTTTCTGACTACATCAGAAAGAGCACCAGAAGG
TTTGTACACGATAAAAATAGGTGAATGA

Upstream 100 bases:

>100_bases
CGTTGGTTAAGGTAAATGACGAGGTTGAAACAAGACGCGGCAAGAAACTTTATCCAGGAGATGTTGTGGAATTTGAAGGA
GAGAAGTACGTAGTCGAATA

Downstream 100 bases:

>100_bases
TATGTATTTGGATATTGGCAACAACACATTAATTGATGAAAAAGAGATTATAGCTGTGGTTAATGTTTCGGACAAATTCT
TCACAGATTTCAAGAAGGAT

Product: DNA replication and repair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIVQKLKLYNYRNFCEIELDFCDGLNLIVGRNASGKTNILEAINVALKGKSFKTNTNSHLIKFGEDEARIVMDVYDDGFE
DKIDITIKSNEKILNVNEAFVNTIKEYNEYFECIVFKPDDLKIIKESKSLRRKFLDESISGVDNYYRTVLKQYNQVLDER
NKLIKNHRYNSYFNEQLKALNIQLSDFGSYIMHKRKSYVERLHLIAKNVCKNLSDENDDLYMENKFSIRYVEDMTDQKNT
YYKSLRDILEKDLENKQTNLGIHRDDLDILINDKQAKFFASQAQVRTAILSMKLAQLDLSRFYNDRMPIILLDDVFSELD
DYRINYIIRYIKDFQAFLTTSERAPEGLYTIKIGE

Sequences:

>Translated_355_residues
MIVQKLKLYNYRNFCEIELDFCDGLNLIVGRNASGKTNILEAINVALKGKSFKTNTNSHLIKFGEDEARIVMDVYDDGFE
DKIDITIKSNEKILNVNEAFVNTIKEYNEYFECIVFKPDDLKIIKESKSLRRKFLDESISGVDNYYRTVLKQYNQVLDER
NKLIKNHRYNSYFNEQLKALNIQLSDFGSYIMHKRKSYVERLHLIAKNVCKNLSDENDDLYMENKFSIRYVEDMTDQKNT
YYKSLRDILEKDLENKQTNLGIHRDDLDILINDKQAKFFASQAQVRTAILSMKLAQLDLSRFYNDRMPIILLDDVFSELD
DYRINYIIRYIKDFQAFLTTSERAPEGLYTIKIGE
>Mature_355_residues
MIVQKLKLYNYRNFCEIELDFCDGLNLIVGRNASGKTNILEAINVALKGKSFKTNTNSHLIKFGEDEARIVMDVYDDGFE
DKIDITIKSNEKILNVNEAFVNTIKEYNEYFECIVFKPDDLKIIKESKSLRRKFLDESISGVDNYYRTVLKQYNQVLDER
NKLIKNHRYNSYFNEQLKALNIQLSDFGSYIMHKRKSYVERLHLIAKNVCKNLSDENDDLYMENKFSIRYVEDMTDQKNT
YYKSLRDILEKDLENKQTNLGIHRDDLDILINDKQAKFFASQAQVRTAILSMKLAQLDLSRFYNDRMPIILLDDVFSELD
DYRINYIIRYIKDFQAFLTTSERAPEGLYTIKIGE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=344, Percent_Identity=24.1279069767442, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41898; Mature: 41898

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVQKLKLYNYRNFCEIELDFCDGLNLIVGRNASGKTNILEAINVALKGKSFKTNTNSHL
CCCCHHHHCCCCCEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCE
IKFGEDEARIVMDVYDDGFEDKIDITIKSNEKILNVNEAFVNTIKEYNEYFECIVFKPDD
EEECCCCCEEEEEECCCCCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHEEEEEECCCC
LKIIKESKSLRRKFLDESISGVDNYYRTVLKQYNQVLDERNKLIKNHRYNSYFNEQLKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NIQLSDFGSYIMHKRKSYVERLHLIAKNVCKNLSDENDDLYMENKFSIRYVEDMTDQKNT
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEHHCCCCHHH
YYKSLRDILEKDLENKQTNLGIHRDDLDILINDKQAKFFASQAQVRTAILSMKLAQLDLS
HHHHHHHHHHHHHCCCHHCCCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFYNDRMPIILLDDVFSELDDYRINYIIRYIKDFQAFLTTSERAPEGLYTIKIGE
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIVQKLKLYNYRNFCEIELDFCDGLNLIVGRNASGKTNILEAINVALKGKSFKTNTNSHL
CCCCHHHHCCCCCEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCE
IKFGEDEARIVMDVYDDGFEDKIDITIKSNEKILNVNEAFVNTIKEYNEYFECIVFKPDD
EEECCCCCEEEEEECCCCCCCEEEEEEECCCEEEECHHHHHHHHHHHHHHEEEEEECCCC
LKIIKESKSLRRKFLDESISGVDNYYRTVLKQYNQVLDERNKLIKNHRYNSYFNEQLKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NIQLSDFGSYIMHKRKSYVERLHLIAKNVCKNLSDENDDLYMENKFSIRYVEDMTDQKNT
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEHHCCCCHHH
YYKSLRDILEKDLENKQTNLGIHRDDLDILINDKQAKFFASQAQVRTAILSMKLAQLDLS
HHHHHHHHHHHHHCCCHHCCCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFYNDRMPIILLDDVFSELDDYRINYIIRYIKDFQAFLTTSERAPEGLYTIKIGE
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA